LOCUS       sequence-173333-        5292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter gene with mouse c-fos promoter. Induced by serum and many 
            growth factors in quiescent cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Cen B, Selvaraj A, Burgess RC, Hitzler JK, Ma Z, Morris SW, Prywes R
  TITLE     Megakaryoblastic leukemia 1, a potent transcriptional coactivator 
            for serum response factor (SRF), is required for serum induction of 
            SRF target genes.
  JOURNAL   Mol Cell Biol. 2003 Sep . 23(18):6597-608.
  PUBMED    12944485
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2003 Sep . 23(18):6597-608."
COMMENT     Sequence Label: pFOS WT-GL3
FEATURES             Location/Qualifiers
     source          1..5292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(65..169)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      196..651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(283..302)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     493..514
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    782..830
                     /note="synthetic polyadenylation signal"
     misc_feature    844..935
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     884..903
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             complement(1428..1439)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      1498..1507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1504..3156
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1570..1588)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3197..3318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3208..3227)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3262..3281
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3566..3583)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3737..4325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3817..3836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4496..5287,5288..5292,1..64))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5119..5138
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
       61 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      121 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      181 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      241 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      301 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      361 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      421 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      481 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      541 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      601 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tgccattcgc
      661 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      721 agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg
      781 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt
      841 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt
      901 ccccagtgca agtgcaggtg ccagaacatt tctctatcga taggtaccga gctcttacgc
      961 gagcttgggc tgcaggtcga ccgccggcga gctgttcccg tcaatccctc cctcctttac
     1021 acaggatgtc catattagga catctgcgtc agcaggtttc cacggccggt ccctgttgtt
     1081 ctgggggggg gaccatctcc gaaatcctac acgcggaagg tctaggagac cccctaagat
     1141 cccaaatgtg aacactcata ggtgaaagat gtatgccaag acgggggttg aaagcctggg
     1201 gcgtagagtt gacgacagag cgcccgcaga gggccttggg gcgcgcttcc ccccccttcc
     1261 agttccgccc agtgacgtag gaagtccatc cattcacagc gcttctataa aggcgccagc
     1321 tgaggcgcct actactccaa ccgcgactgc agcgagcaac tgagaagact ggatagagcc
     1381 ggcggttccg cgaacgagca gtgaccgcgc tcccacccag ctctgctctg cagctcccac
     1441 cagtgtctct ctagcccggg ctcgagatca gcttggcatt ccggtactgt tggtaaagcc
     1501 accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
     1561 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
     1621 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
     1681 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
     1741 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
     1801 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
     1861 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
     1921 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
     1981 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
     2041 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
     2101 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
     2161 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
     2221 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     2281 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat
     2341 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
     2401 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
     2461 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
     2521 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     2581 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     2641 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
     2701 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     2761 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
     2821 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
     2881 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
     2941 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     3001 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     3061 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     3121 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg
     3181 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3241 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3301 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3361 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
     3421 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     3481 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     3541 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3601 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4141 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4381 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4441 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4501 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4561 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4621 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4681 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4741 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4801 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5101 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5281 ctgggtgagc aa
//
