LOCUS       sequence-173334-        8400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary DEST vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Nowack MK, Grini PE, Jakoby MJ, Lafos M, Koncz C, Schnittger A
  TITLE     A positive signal from the fertilization of the egg cell sets off 
            endosperm proliferation in angiosperm embryogenesis.
  JOURNAL   Nat Genet. 2006 Jan;38(1):63-7. Epub 2005 Nov 27.
  PUBMED    16311592
REFERENCE   2  (bases 1 to 8400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2006-01"; volume: "38(1)"; pages: "63-7. Epub 2005 
            Nov 27"
COMMENT     Sequence Label: pAM-PAT-ProCDKA;1 GW
FEATURES             Location/Qualifiers
     source          1..8400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    348..372
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      484..1193
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1266..1357
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1358..1426,1427..2218)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1576..1595)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2389..2977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2878..2897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3289..3313
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3407..3659)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3679..4230)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4272..4451)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    6673..6797
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6822..6827,6828..6845,6846..6852)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6906..7565
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6972..6991)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7907..8212
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8091..8110
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8253..8377)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agcttgttga gaattcatcg cccgggactg ggttaacttc cgatcgattc tagactagtt
       61 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
      121 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
      181 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
      241 taaaatttct aattcctaaa accaaaatcc agtgaccggg cggccgccac cgcggtggag
      301 ggggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
      361 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
      421 tgaaaaggtt tatccgttcg tccatttgta tgtgtacatc accgacgagc aaggcaagac
      481 cgagcgcctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct
      541 atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cggccgccgg
      601 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
      661 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
      721 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
      781 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
      841 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
      901 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
      961 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     1021 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     1081 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
     1141 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc
     1201 agtccttgcc attgccggga tcggggcagt aacgggatgg gcgatcagcc cgaaagctac
     1261 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1321 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1381 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1441 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1501 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1561 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1621 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1681 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1741 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1801 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1861 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1921 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1981 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2041 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2101 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2161 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2521 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3001 acggttcctg gccttttgct ggccttttgc tcacatggac tctagctaga ggatcacagg
     3061 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
     3121 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
     3181 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
     3241 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
     3301 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
     3361 gatcgaatac taacgtctct accagatatc agcttgcatg ccggtcgatc tagtaacata
     3421 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     3481 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     3541 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     3601 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
     3661 gcttgactct aggggtcatc agatttcggt gacgggcagg accggacggg gcggcaccgg
     3721 caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct tgaagccggc
     3781 cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca cgctcgggtc
     3841 gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca gggacttcag
     3901 caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg agacgtacac
     3961 ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc cagggacccg cgtaggcgat
     4021 gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc gcagacggac
     4081 gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga ccgtgcttgt
     4141 ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg tggcacggcg
     4201 gatgtcggcc gggcgtcgtt ctgggctcat ggtagatccc ctcgatcgag ttgagagtga
     4261 atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga
     4321 caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt
     4381 tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctcagtg gctccttcaa
     4441 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
     4501 ataacgtgac tcccttaatt ctccgctcat gatcagattg tcgtttcccg ccttcggttt
     4561 gggcgcgccg ggaagataga agggaagaga gaggtagacg gaaagagaaa tggagaaaaa
     4621 tagataaagg ggtaaacttg gaattaatat acaaaatata gaggatatca gttctttttc
     4681 cttttgatgt tagtttctta attataaact ttttgttggt tagttttgcc aattccctta
     4741 cttacaactg ataaccgtat agctctttta tcagaaaaaa ctttatatat ctaatctccc
     4801 aaaatataca aatgtgagaa atatctgaaa ataaatcaat tctcaccaat tatatatatt
     4861 tgattactta aaaatgttaa tatatggcag ttgcttgttc agagaataac ttaaaaaatt
     4921 gatcacgaga tcggcttatg tttaatattt ttgaactctt gggataaaag ttttgaagat
     4981 atatatatcg tagaaaagtt ttgtgttctt taattagtta caaaattttg agtttttttt
     5041 ttttctttca taaagtatca gaatatcagg gtgatttatt gaatagttta ttttttctcg
     5101 ttctactcac gttattcttg caagtattaa ttttggtttg tttataaaaa aagtatttgg
     5161 aaattttatg tgagcaagtg cgaaataaac gaggattttg ggaagtattt ttttttttgt
     5221 aaaatcggtt atattgaacg ttatactata tatattatta tataaatata actgctaaag
     5281 actaaattta gatactaaaa tcagcattga tgaagatcca catgaataag actcgtctct
     5341 taactcaaca tttaacatat gattttcata ttattgatca tttatctttg tgttggtcca
     5401 aggtgccaga ctttattatt tacattaaat gttgagttgt ttactgacat attttttaat
     5461 taaaacaact ttcatattat tgcccattta tattttggtt ggtcctccaa agacttacag
     5521 gatacatatg tttctccatt ttcattttta tgatctaaaa cgtctttttc taacttagat
     5581 tctaaatggt tacagcaaac cggacataca attttgcttg tcaaatggat cgttgagata
     5641 gacaacaatc caatgtaaaa ccaaaactct tgatttataa ttggtctgca atgatttttt
     5701 tttggttcat ataccatttt ataagatagc aaaccatttt catcataaaa gatatatttt
     5761 tttatcattg aataccaatc aaacccggta taataaataa aattgtacca aattaatgcg
     5821 gttaggtaaa ttcaatttat tggaaattaa agaagagaaa aaaaaaataa cgaacaaaat
     5881 ttccttgaca caaaaaaaaa aaaagaagaa agaaaaaacc cttactctca attagtcaat
     5941 ccccttccct agtcctcctc tcttcaagct tttcacagaa aaccaccacc cttctctctc
     6001 tactgccttt ttaccacaga gaagagagag gatccgtcgg tgtgctagtc tcactgacac
     6061 tacatccgat cgtcgccggt aagttgattt cgttactctc cgatcaattt cttttccgga
     6121 ggaggaactt gttttccgtg cgaaattctg acttgaactt tgttgtcaat tcatcaaacg
     6181 ctttatcttt tctccatctt caattatctt actgcttcgt cgctgatctc tacttgtttt
     6241 cctccgtttt gcattttcag tggcgtttct tagtgtttca ctagatttag actggttttg
     6301 cttgtgtgca ttttgtcatc agtgtttatg attgttcgct ttagctctat gtttctatgg
     6361 atttgataat atatgagttc gctgctctgg attcagctgg cttgtttgat tgttcatttt
     6421 cataattctg tccaaagaat caaatccaca gtagctcgga tctgagattg atttgtttct
     6481 ttgcgtcttt gtgtagattt gactttaatt ttgagattgt tgtgtgctga tatacagctt
     6541 aattaatata gcctctgatg tgttattatc gatgtgtcat tatcaatcta caggtgacat
     6601 tttataagtg tggagtttac ttcagcttta ttattcagga attgctcgag aaagaggatc
     6661 cacctgagga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat
     6721 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
     6781 catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg
     6841 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag
     6901 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     6961 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     7021 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     7081 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
     7141 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     7201 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     7261 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     7321 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     7381 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     7441 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     7501 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     7561 cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
     7621 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta
     7681 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
     7741 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     7801 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     7861 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt
     7921 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     7981 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     8041 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     8101 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     8161 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     8221 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
     8281 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     8341 tttacgtttc tcgttcagct ttcttgtaca aagtggtgat cctccgttcc atgggctaga
//
