LOCUS       sequence-173335-        9869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express iCre, tdTomato and puromycin resistance gene (puromycin 
            N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9869)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-CB-iCre-P2A-tdTomato-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..9869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          182..278
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             380..400
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1442..1498
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1499..2926
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGIGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             2948..3001
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3002..3601
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3002..3021)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3498..3518
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3614..4202
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3667..3687)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4085..4096)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4276..4509
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4581..4702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4618..4637)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4672..4691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4721..4856
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4889..4905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4889..4905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4902..4924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4913..4929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4937..4943,4944..4961,4962..4967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4982..5003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5120..5137)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5291..5879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6050..6841,6842..6910))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6673..6692
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6911..7015)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7083..7101
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7139..7161)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7261..7280
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7474..7496
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7488..7505
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7489..7505
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7520..7746
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7747..7927
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7974..8099
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8592..8825
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9010..9054
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9321..9437
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
       61 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagcgggat
      121 cagccaccgc ggtggcggcc ctagagtcga tcgaggaact gaaaaaccag aaagttaact
      181 ggtaagttta gtctttttgt cttttatttc aggtccccga tccggtggtg gtgcaaatca
      241 aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa gtgttacttc
      301 tgctctaaaa gctgcggaat tgtacccgcg gccgatccac cggtcgggat ccttcgaaac
      361 cggtgctagc accatggtgc ccaagaagaa gaggaaagtc tccaacctgc tgactgtgca
      421 ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat
      481 ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt
      541 gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc
      601 tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat
      661 ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc
      721 tgactccaat gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg
      781 ggagagagcc aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct
      841 gatggagaac tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta
      901 caacaccctg ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac
      961 cgatggtggg agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg
     1021 tgtggagaag gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc
     1081 tggtgtggct gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc
     1141 tgccccttct gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac
     1201 ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg
     1261 ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga
     1321 aatcatgcag gctggtggct ggaccaatgt gaacattgtg atgaactaca tcagaaacct
     1381 ggactctgag actggggcca tggtgaggct gctcgaggat ggggacgtta acggaagcgg
     1441 agccactaac ttctccctgt tgaaacaagc aggggatgtc gaagagaatc ccgggccaat
     1501 ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     1561 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     1621 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     1681 cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     1741 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     1801 ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     1861 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     1921 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     1981 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     2041 ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga
     2101 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     2161 ccaccacctg ttcctggggc atggcatcgg cagcaccggc agcggcagct ccggcaccgc
     2221 ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca aggtgcgcat
     2281 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     2341 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     2401 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
     2461 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2521 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     2581 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     2641 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     2701 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     2761 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
     2821 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     2881 gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaaggtcg acaagcttgg
     2941 cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc
     3001 aatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     3061 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     3121 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     3181 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     3241 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     3301 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     3361 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     3421 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     3481 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     3541 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     3601 agaattcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3661 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3721 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3781 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     3841 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     3901 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3961 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     4021 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4081 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4141 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4201 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4261 aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg
     4321 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4381 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4441 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4501 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4561 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     4621 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4681 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     4741 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4801 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     4861 cttttgcaga gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
     4921 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4981 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5101 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5161 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5221 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5281 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5341 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5401 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5461 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5521 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5581 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5641 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5701 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     5761 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5821 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5881 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5941 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6001 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6061 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6121 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6181 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6241 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6301 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6361 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6421 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6481 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6541 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6601 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6661 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6721 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6781 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6841 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6901 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6961 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7021 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     7081 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     7141 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7201 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     7261 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     7321 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     7381 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     7441 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     7501 ccagtgccaa gctgacgcgt gtagtcttat gcaatactct tgtagtcttg caacatggta
     7561 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     7621 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     7681 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     7741 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7801 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7861 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7921 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     7981 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     8041 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     8101 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     8161 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     8221 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     8281 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     8341 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     8401 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cactgatctt cagacctgga
     8461 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     8521 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     8581 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     8641 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     8701 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     8761 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     8821 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     8881 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     8941 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     9001 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     9061 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     9121 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     9181 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     9241 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     9301 tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     9361 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     9421 acaaaattca aaattttatc gattaattaa ggtacctctg gtcgttacat aacttacggt
     9481 aaatggcccg cctggctgac cgcccaacga ccccgcccat tgacgtcaat aatgacgtat
     9541 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     9601 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     9661 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     9721 cctacttggc agtacatcta ctcgaggcca cgttctgctt cactctcccc atctcccccc
     9781 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
     9841 cggggggggg gggggggggg gcgcgcgcc
//
