LOCUS       sequence-173339-        1194 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 
            375-591) plus constitutive expression of mCherry in mammalian cells 
            (Dual-promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Harterink M, da Silva ME, Will L, Turan J, Ibrahim A, Lang AE, van 
            Battum EY, Pasterkamp RJ, Kapitein LC, Kudryashov D, Barres BA, 
            Hoogenraad CC, Zuchero JB
  TITLE     DeActs: genetically encoded tools for perturbing the actin 
            cytoskeleton in single cells.
  JOURNAL   Nat Methods. 2017 Apr 10. doi: 10.1038/nmeth.4257.
  PUBMED    28394337
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Apr 10. doi: 10.1038/nmeth.4257."
COMMENT     Sequence Label: pTetON-DHFRdd-SpvB; CMV-mCherry
FEATURES             Location/Qualifiers
     source          1..1194
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..489
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             1162..1188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggatccgcca ccatgatcag tctgattgcg gcgttagcgg tagattacgt tatcggcatg
       61 gaaaacgcca tgccgtggaa cctgcctgcc gatctcgcct ggtttaaacg caacacctta
      121 aataaacccg tgattatggg ccgccatacc tgggaatcaa tcggtcgtcc gttgccagga
      181 cgcaaaaata ttatcctcag cagtcaaccg agtacggacg atcgcgtaac gtgggtgaag
      241 tcggtggatg aagccatcgc ggcgtgtggt gacgtaccag aaatcatggt gattggcggc
      301 ggtcgcgtta ttgaacagtt cttgccaaaa gcgcaaaaac tgtatctgac gcatatcgac
      361 gcagaagtgg aaggcgacac ccatttcccg gattacgagc cggatgactg ggaatcggta
      421 ttcagcgagt tccacgatgc tgatgcgcag aactctcaca gctattgctt tgagattctg
      481 gagcggcgag aattcggatc cggatccgga tccggaggta attcatctcg accaaaatca
      541 aaatgggcga ttgtagaggc atcaaagcag attcaagctc tgaggtacta ttcagctcaa
      601 gggtacagtg tgattaataa atatttacgt ggggatgatt atcctgaaac acaggcaaaa
      661 gaaactctgc tctccagaga ctatctttcc acaaatgaac ccagtgatga ggagtttaaa
      721 aatgccatgt cagtttatat aaatgatatt gcggagggat taagttcact tcccgaaaca
      781 gatcacagag tcgtataccg gggcctgaag cttgataagc ccgcattatc ggatgtgctg
      841 aaggaataca ctactatagg taatataata atagataaag cttttatgag tacatcgcca
      901 gataaggcat ggataaatga cactattctc aacatatacc tagaaaaagg acataaaggt
      961 agaatactcg gagatgttgc acattttaag ggagaggcag agatgctttt ccctccaaat
     1021 actaaactca aaatcgaaag cattgtaaat tgtggatccc aagactttgc aagccagctt
     1081 agtaagctga gattaagtga tgatgcaact gctgacacaa acaggataaa aagaataata
     1141 aacatgaggg tactcaactc atacccatac gatgttccag attacgcttg ataa
//
