LOCUS       sequence-173340-        1573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 
            375-591) plus constitutive expression of mCherry in mammalian cells 
            (Dual-promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Harterink M, da Silva ME, Will L, Turan J, Ibrahim A, Lang AE, van 
            Battum EY, Pasterkamp RJ, Kapitein LC, Kudryashov D, Barres BA, 
            Hoogenraad CC, Zuchero JB
  TITLE     DeActs: genetically encoded tools for perturbing the actin 
            cytoskeleton in single cells.
  JOURNAL   Nat Methods. 2017 Apr 10. doi: 10.1038/nmeth.4257.
  PUBMED    28394337
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Apr 10. doi: 10.1038/nmeth.4257."
COMMENT     Sequence Label: pTetON-DHFRdd-SpvB; CMV-mCherry
FEATURES             Location/Qualifiers
     source          1..1573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1..122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12..31)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     66..85
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(245..955)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(523..542)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     792..810
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      952..961
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    963..1043
                     /label=MCS
                     /note="multiple cloning site"
     promoter        complement(1066..1269)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1095..1115)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1270..1573
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
       61 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      121 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      181 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc
      241 cgctttactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt
      301 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt
      361 tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt
      421 cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt
      481 cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg
      541 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
      601 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
      661 cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct
      721 tcacgtaggc cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca
      781 gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct
      841 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
      901 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatggtgg
      961 cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcgaagc ttgagctcga
     1021 gatctgagtc cggtagcgct agcggatctg acggttcact aaaccagctc tgcttatata
     1081 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
     1141 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
     1201 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
     1261 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     1321 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     1381 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     1441 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     1501 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
     1561 aagttatgta acg
//
