LOCUS       sequence-173341-       10357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DSG-2 tension sensor, using TSmod inserted in human DSG-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Baddam SR, Arsenovic PT, Narayanan V, Duggan NR, Mayer CR, Newman 
            ST, Abutaleb DA, Mohan A, Kowalczyk AP, Conway DE
  TITLE     The Desmosomal Cadherin Desmoglein-2 Experiences Mechanical Tension 
            as Demonstrated by a FRET-Based Tension Biosensor Expressed in 
            Living Cells.
  JOURNAL   Cells. 2018 Jun 26;7(7). pii: cells7070066. doi: 
            10.3390/cells7070066.
  PUBMED    29949915
REFERENCE   2  (bases 1 to 10357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2018-06-26"; volume: "7(7). pii: cells7070066. doi"; pages: "
            10.3390/cells7070066"
COMMENT     Sequence Label: pcdna DSG-2 TS
FEATURES             Location/Qualifiers
     source          1..10357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3205..3912
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4033..4035,4036..4038,4039..4749)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4078..4099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4339..4358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4686..4707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5951..5968)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5957..6181
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6227..6655
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6314..6333)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6524..6545
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6664..6684)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6669..6998
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6849..6984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6911..6930
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7065..7859
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7119..7138)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7729..7748
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8033..8154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8070..8089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8124..8143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8203..8219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8203..8219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8216..8238)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8227..8243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8251..8257,8258..8275,8276..8281))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8296..8317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8434..8451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8605..9190)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8685..8704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9361..10152,10153..10221))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9984..10003
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10222..10326)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatccag cacagtggcg gccgctcgag ggtgcgatgg cgcggagccc gggacgcgcg
     1021 tacgccctgc tgcttctcct gatctgcttt aacgttggaa gtggacttca cttacaggtc
     1081 ttaagcacaa gaaatgaaaa taagctgctt cctaaacatc ctcatttagt gcggcaaaag
     1141 cgcgcctgga tcaccgcccc cgtggctctt cgggagggag aggatctgtc caagaagaat
     1201 ccaattgcca agatacattc tgatcttgca gaagaaagag gactcaaaat tacttacaaa
     1261 tacactggaa aagggattac agagccacct tttggtatat ttgtctttaa caaagatact
     1321 ggagaactga atgttaccag cattcttgat cgagaagaaa caccattttt tctgctaaca
     1381 ggttacgctt tggatgcaag aggaaacaat gtagagaaac ccttagagct acgcattaag
     1441 gttcttgata tcaatgacaa cgaaccagtg ttcacacagg atgtctttgt tgggtctgtt
     1501 gaagagttga gtgcagcaca tactcttgtg atgaaaatca atgcaacaga tgcagatgag
     1561 cccaataccc tgaattcgaa aatttcctat agaatcgtat ctctggagcc tgcttatcct
     1621 ccagtgttct acctaaataa agatacagga gagatttata caaccagtgt taccttggac
     1681 agagaggaac acagcagcta cactttgaca gtagaagcaa gagatggcaa tggagaagtt
     1741 acagacaaac ctgtaaaaca agctcaagtt cagattcgta ttttggatgt caatgacaat
     1801 atacctgtag tagaaaataa agtgcttgaa gggatggttg aagaaaatca agtcaacgta
     1861 gaagttacgc gcataaaagt gttcgatgca gatgaaatag gttctgataa ttggctggca
     1921 aattttacat ttgcatcagg aaatgaagga ggttatttcc acatagaaac agatgctcaa
     1981 actaacgaag gaattgtgac ccttattaag gaagtagatt atgaagaaat gaagaatctt
     2041 gacttcagtg ttattgtcgc taataaagca gcttttcaca agtcgattag gagtaaatac
     2101 aagcctacac ccattcccat caaggtcaaa gtgaaaaatg tgaaagaagg cattcatttt
     2161 aaaagcagcg tcatctcaat ttatgttagc gagagcatgg atagatcaag caaaggccaa
     2221 ataattggaa attttcaagc ttttgatgag gacactggac taccagccca tgcaagatat
     2281 gtaaaattag aagatagaga taattggatc tctgtggatt ctgtcacatc tgaaattaaa
     2341 cttgcaaaac ttcctgattt tgaatctaga tatgttcaaa atggcacata cactgtaaag
     2401 attgtggcca tatcagaaga ttatcctaga aaaaccatca ctggcacagt ccttatcaat
     2461 gttgaagaca tcaacgacaa ctgtcccaca ctgatagagc ctgtgcagac aatctgtcac
     2521 gatgcagagt atgtgaatgt tactgcagag gacctggatg gacacccaaa cagtggccct
     2581 ttcagtttct ccgtcattga caaaccacct ggcatggcag aaaaatggaa aatagcacgc
     2641 caagaaagta ccagtgtgct gctgcaacaa agtgagaaaa agcttgggag aagtgaaatt
     2701 cagttcctga tttcagacaa tcagggtttt agttgtcctg aaaagcaggt ccttacactc
     2761 acagtttgtg agtgtctgca tggcagcggc tgcagggaag cacagcatga ctcctatgtg
     2821 ggcctgggac ccgcagcaat tgcgctcatg attttggcct ttctgctcct gctattggta
     2881 ccacttttac tgctgatgtg ccattgcgga aagggcgcca aaggctttac ccccatacct
     2941 ggcaccatag agatgctgca tccttggaat aatgaaggag caccacctga agacaaggtg
     3001 gtgccatcat ttctgccagt ggatcaaggg ggcagtctag taggaagaaa tggagtagga
     3061 ggtatggcca aggaagccac gatgaaagga agtagctctg cttccattgt caaagggcaa
     3121 catgagatgt ccgagatgga tggaaggtgg gaagaacaca gaagcctgct ttctggtaga
     3181 gctacccagt ttacaggggt cgagatggtg agcaagggcg aggagaccac aatgggcgta
     3241 atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc
     3301 gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag
     3361 gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac
     3421 ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc
     3481 cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg
     3541 aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag
     3601 aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc
     3661 gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg
     3721 gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa gaaggcggtg
     3781 aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca cgacaaggac
     3841 tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga cggcatggac
     3901 gagctgtaca aggggccagg tggtgcaggg ccaggtggtg cagggccagg tggtgcaggg
     3961 ccaggtggtg cagggcccgg tggtgcaggt ccaggtggtg caggtccagg tggtgcaggt
     4021 ccaggtggtg ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     4081 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     4141 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     4201 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
     4261 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4321 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4381 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4441 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     4501 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     4561 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4621 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     4681 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4741 ctgtacaagg cggccgcggc cacaggcgct atcatgacca ctgaaaccac gaagaccgca
     4801 agggccacag gggcttccag agacatggcc ggagctcagg cagctgctgt tgcactgaac
     4861 gaagaattct taagaaatta tttcactgat aaagcggcct cttacactga ggaagatgaa
     4921 aatcacacag ccaaagattg ccttctggtt tattctcagg aagaaactga atcgctgaat
     4981 gcttctattg gttgttgcag ttttattgaa ggagagctag atgaccgctt cttagatgat
     5041 ttgggactta aattcaagac actagctgaa gtttgcctgg gtcaaaaaat agatataaat
     5101 aaggaaattg agcagagaca aaaacctgcc acagaaacaa gtatgaacac agcttcacat
     5161 tcactctgtg agcaaactat ggttaattca gagaatacct actcctctgg cagtagcttc
     5221 ccagttccaa aatctttgca agaagccaat gcagagaaag taactcagga aatagtcact
     5281 gaaagatctg tgtcttctag gcaggcgcaa aaggtagcta cacctcttcc tgacccaatg
     5341 gcttctagaa atgtgatagc aacagaaact tcctatgtca cagggtccac tatgccacca
     5401 accactgtga tcctgggtcc tagccagcca cagagcctta ttgtgacaga gagggtgtat
     5461 gctccagctt ctaccttggt agatcagcct tatgctaatg aaggtacagt tgtggtcact
     5521 gaaagagtaa tacagcctca tgggggtgga tcgaatcctc tggaaggcac tcagcatctt
     5581 caagatgtac cttacgtcat ggtgagggaa agagagagct tccttgcccc cagctcaggt
     5641 gtgcagccta ctctggccat gcctaatata gcagtaggac agaatgtgac agtgacagaa
     5701 agagttctag cacctgcttc cactctgcaa tccagttacc agattcccac tgaaaattct
     5761 atgacggcta ggaacaccac ggtgtctgga gctggagtcc ctggccctct gccagatttt
     5821 ggtttagagg aatctggtca ttctaattct accataacca catcttccac cagagttacc
     5881 aagcatagca ctgtacagca ttcttactcc taactcgagt ctagagggcc cgtttaaacc
     5941 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     6001 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     6061 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6121 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6181 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     6241 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6301 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6361 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6421 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6481 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6541 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6601 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     6661 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6721 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6781 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6841 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6901 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6961 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7021 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     7081 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     7141 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     7201 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     7261 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     7321 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     7381 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     7441 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     7501 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     7561 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     7621 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7681 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7741 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7801 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     7861 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     7921 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7981 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     8041 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8101 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8161 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     8221 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8281 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8341 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8401 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9121 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9181 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9241 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9301 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9361 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9421 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9481 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9541 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9601 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9661 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9721 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9781 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9841 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9901 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9961 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10021 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10081 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10141 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10201 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10261 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10321 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
