LOCUS       sequence-173347-        4982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TRAPPC10_pCSdest
FEATURES             Location/Qualifiers
     source          1..4982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(833..857)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    901..1035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(925..944)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     979..998
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1148..1168)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1148..1166)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1187..1203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1187..1203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1200..1222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1211..1227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1235..1241,1242..1259,1260..1265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1280..1301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1418..1435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1589..2177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1669..1688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2348..3139,3140..3208))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2971..2990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3209..3313)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3339..3794)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3476..3497)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3688..3707
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3865..4849
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        4884..4902
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4884..4901
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    4956..4980
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggacgcct ctgaggagcc gctgccgccg gtgatctaca ccatggagaa caagcccatc
       61 gtcacctgtg ctggagatca gaatttattt acctctgttt atccaacgct ctctcagcag
      121 cttccaagag aaccaatgga atggagaagg tcctatggcc gggctccgaa gatgattcac
      181 ctagagtcta actttgttca attcaaagag gagctgctgc ccaaagaagg aaacaaagct
      241 ctgctcacgt ttcccttcct ccatatttac tggacagagt gctgtgatac cgaagtgtat
      301 aaagctacag taaaagatga cctcaccaag tggcagaatg ttctgaaggc tcatagctct
      361 gtggactggt taatagtgat agttgaaaat gatgccaaga aaaaaaacaa aaccaacatc
      421 cttccccgaa cctctattgt ggacaaaata agaaatgatt tttgtaataa acagagtgac
      481 aggtgtgttg tgctctccga ccccttgaag gactcttctc gaactcagga atcctggaat
      541 gccttcctga ccaaactcag gacattgctt cttatgtctt ttaccaaaaa cctaggcaag
      601 tttgaggatg acatgagaac cttgagggag aagaggactg agccaggctg gagcttttgt
      661 gaatatttca tggttcagga ggagcttgcc tttgttttcg agatgctgca gcagttcgag
      721 gacgccctgg tgcagtacga cgaactggac gccctcttct ctcagtatgt ggtcaacttc
      781 ggggccgggg gtataaaatg tcctttccac aactcagtgg cctgctggta gaacccagct
      841 ttcttgtaca aagtggtgat cctctcgagc ctctagaact atagtgagtc gtattacgta
      901 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      961 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     1021 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     1081 gtgtgggagg ttttttaatt cgcggccgcg gcgccaatgc attgggcccg gtacccagct
     1141 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     1201 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccggg agcataaagt
     1261 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1321 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1381 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1441 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1501 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1561 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1621 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1681 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1741 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1801 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1861 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1921 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1981 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2041 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2101 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2161 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2221 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2281 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2341 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2401 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2461 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2521 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2581 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2641 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2701 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2761 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2821 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2881 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2941 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3001 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3061 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3121 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3181 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3241 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3301 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     3361 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3421 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3481 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3541 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3601 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3661 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3721 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3781 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     3841 gcgggcctct tcgctattac gccagtcgac cgccaattca atatggcgta tatggactca
     3901 tgccaattca atatggtgga tctggacctg tgccaattca atatggcgta tatggactcg
     3961 tgccaattca atatggtgga tctggacccc agccaattca atatggcgga cttggcacca
     4021 tgccaattca atatggcgga cctggcactg tgccaactgg ggaggggtct acttggcacg
     4081 gtgccaagtt tgaggagggg tcttggccct gtgccaagtc cgccatattg aattggcatg
     4141 gtgccaataa tggcggccat attggctata tgccaggatc aatatatagg caatatccaa
     4201 tatggcccta tgccaatatg gctattggcc aggttcaata ctatgtattg gccctatgcc
     4261 atatagtatt ccatatatgg gttttcctat tgacgtagat agcccctccc aatgggcggt
     4321 cccatatacc atatatgggg cttcctaata ccgcccatag ccactccccc attgacgtca
     4381 atggtctcta tatatggtct ttcctattga cgtcatatgg gcggtcctat tgacgtatat
     4441 ggcgcctccc ccattgacgt caattacggt aaatggcccg cctggctcaa tgcccattga
     4501 cgtcaatagg accacccacc attgacgtca atgggatggc tcattgccca ttcatatccg
     4561 ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc cacttggcag tacatcaata
     4621 tctattaata gtaacttggc aagtacatta ctattggaag tacgccaggg tacattggca
     4681 gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat ggctgccaag tacatcccca
     4741 ttgacgtcaa tggggagggg caatgacgca aatgggcgtt ccattgacgt aaatgggcgg
     4801 taggcgtgcc taatgggagg tctatataag caatgctcgt ttagggaacc gccattctgc
     4861 ctggggacgt cggagcaagc ttgatttagg tgacactata gaatacaagc tacttgttct
     4921 ttttgcagga tcccatcgat tcgaattcaa ggatcacaag tttgtacaaa aaagcaggca
     4981 cc
//
