LOCUS       sequence-173350-       10742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses efflux pump in E. coli to provide resistance to biofuel 
            (low copy).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10742)
  AUTHORS   Turner WJ, Dunlop MJ
  TITLE     Trade-Offs in Improving Biofuel Tolerance Using Combinations of 
            Efflux Pumps.
  JOURNAL   ACS Synth Biol. 2015 Oct 16;4(10):1056-63. doi: 10.1021/sb500307w. 
            Epub 2014 Dec 12.
  PUBMED    25496359
REFERENCE   2  (bases 1 to 10742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500307w";
            journalName: "ACS Synth Biol"; date: "2015-10-16- 16"; volume: "4"; 
            issue: "10"; pages: "1056-63"
COMMENT     Sequence Label: pBbS5c-Maqu_0582
FEATURES             Location/Qualifiers
     source          1..10742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(49..1131)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1093..1112
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1132..1209)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1415..1434
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(1520..1525,1526..1543,1544..1550)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1558..1574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      7485..7556
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7572..7599
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(8130..9080)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(9842..9936)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9957..10616)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     10531..10550
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(10617..10719)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atcttttaag aaggagatat acatatgcag
     1621 acagagaaga gtttgatggt gcggctattt ggggtccgca tgttgccaat gtggttggcg
     1681 gttcttttgc tggctggttg tgacaattcc tccggtcagg caaaggctga acggtcagcg
     1741 ccgccgcctc cggaagtcgg ggttgttgaa atgcagccgc agcgtgtgaa cctttcaatg
     1801 gaactgccgg gcaggaccgc cgcttaccgt atctcggaag tgaggccgca agtcacgggc
     1861 attttgcagg agcgcttgtt tgaacaaggc gcccaggtca aagcaggtga ggtcctctac
     1921 cagatcgacc ccaaccgata ccgtgctgcc catcaacggg cccaggcaga actggagcgc
     1981 gcacaggccg aattgcgaca ggctcagcgg gagtgggcac gtacgtcaga cttgtttgag
     2041 aagaatgcgg tcagcaagcg tcagcgtgat gaagcgctat cggccttgga ggcagcccgg
     2101 gcggatgtct cccgatccga ggcgatggtc gaaacggcgg gtatcgagct ggcgtatacc
     2161 gaggtcaagg ctcccatcgc cgggcgcatt ggcccgacac tgtacacgga gggtgcactg
     2221 gtaaccgcca atcagcccca ggtcctggcg cgcgtggttc agcttgatcc catgtatgtc
     2281 gatattcagc taccggtcga aaaactggct cgaattcggt cgtctctgaa tgagggcgcg
     2341 gcttctaagc ccggcctaaa tgaagccgag actgttttgt tgcgcgatga cggtactctc
     2401 tatccgcata agggacgcgt tgacgttgcg gatgttaccg tcaaccaaag caccagttcg
     2461 gtcactgtgc gggcggtttt ccccaatcct gacgaagacc ttctgcctgg aatgtacgtg
     2521 cgcgttcgcc tgagcgaggg ggtgcgggag aacgcccttc tggcgccgca gcagggcgtg
     2581 agccgtaatc cgcaaggcga ggccacggcg ttgctggtca acccgcaggg agaagttgtt
     2641 gcccgccagt tagaggcaga gcgggccatt ggcgctttct ggctggtgga gaacgggtta
     2701 gagccggggg atcgccttat cgtctccggt ctgcaaaagg ttcggccagg ggccaaggtg
     2761 aagccggttg aggcagatgt tccattacat cccacccaaa accagcaagc cagtggcaag
     2821 gatagttcac agaaaagcgc ctccgacgac gagggccccg cacaatgatt aatttcttca
     2881 tttccaggcc cgttttttcc tgggttctgg ccattgtcgc catgttggcg ggcatcatgg
     2941 cgatcttcgt tttgccggtc cagcgatacc cgtcggtggc gccgccggcg gtggagattc
     3001 aggccgatta tcctggcgcc tcggcggaca ccgtgtccaa tacggtggtt caggtcattg
     3061 aacaagagat gaccggcctc gacaatttgc tctatatggg gtcaacagcg gattcctcag
     3121 gccacgctac ggttactctc acctttgcgg cgggcaccga tccggacatt gcccaggtcc
     3181 aggtgcagaa caagctcaaa ctggcggaac cgcggctccc ggaggtggtg cgccagcagg
     3241 ggatcagcgt cgagaaatcc agcaccagtt ttctgatggt aatggcattc gtctctaccg
     3301 acggtcgcct cagcaagctg gatatcgccg attttatcag ctccgagtta gccgaaccca
     3361 ttggacgggt aactggtatc ggcagcgtgc aggttttcgg ctctgaatat gcgatgcgga
     3421 tttggctgga cccttcggcg ttgaccaatt acggcctgac cgtcgccgac gtgagcgccg
     3481 ccatcgaggc gcaaaacgcc caggtcaccg ccggccagct cggcgggctg cccgcggtcg
     3541 agggtcagca gctcaatgcc acggtcaccg cccagacctt gctcacctca accgaagagt
     3601 tccgcaacat cctgctcaag agtatgcccg acgggtcccg ggtgcgcctg ggggatgttg
     3661 cccgcgttga gctgggcggc ggtgcagtcc agattgacac tttctacgat ggcgagccgg
     3721 cagccggcct tggcatcaat ctggcgccgg gtgccaactc gcttgcggtc acagaggctg
     3781 ttaaagcgcg gctccaggag ctcgagccgt attttccgga aggggtggag attcggtatc
     3841 cctaccagac ggcccccttc gtggaggcct ccatcgacgc ggtggtcaca accatcatcg
     3901 aggcaatcgc cctggtggtc ctggtcatgc tggtatttct ccagagttgg cgggctaccc
     3961 tgataccagc gatcgcaatc ccggtggtgc tgctgggcac ctttgcgatc atggcggcct
     4021 tcggcttttc catcaacatg ctcactctct tcggtctcgt attggcgatc ggccttctgg
     4081 tggatgacgc catcgtggtg gttgagaacg tcgagcgtgt gatgcatgag gacggcctga
     4141 gcccgatgga ggcgacgcgc aagtccatgg ggcagatagc cagcgcgctg attggcatcg
     4201 gcgtggtgtt gtctgcggtg tttatcccga tggccttctt tccgggctcc accggggcaa
     4261 tctatcggca gttttccctg agtattgctg gtgccatggt gctttcggtg ctggtggctc
     4321 tgattctgag ccccatgctg tgtggtcgga tcctcaagcc cacgcatgaa agaaccttgc
     4381 tgcaaaaact gttcggctgg tttgaggcag gcctggggcg actgacccgg ggctatgtcc
     4441 gcctggttgg ccatacggcg cgccacgcct ggctctacac actggcgttc ctgggcatcg
     4501 tcggcctggt ggccgttctc tttgtgcggt tgcccggcgg gttcctgccc gcggaagacc
     4561 aggggttcgc cgtggtgcag tatcagttgc ccgccggtgc gacccagcag cgcacaatcg
     4621 atacgattca ggttatcgaa gattacttca tggaccagga tgaggtgcag ggcctcttca
     4681 caatcgctgg tttcagcttc gctggccggg cgcagaacgc gggactggcg ttcgtcaatc
     4741 tcaagccatg gagtgagcgc gatcccgaga cgcaaagcgc cgacgcgatc attcagcggg
     4801 cgaaccgggc gcttgccggg ctggtgcgtg atgggcgggc ttttgctttc aatttgccac
     4861 cgataccgga attggggcag gccctgggct ttgaattgcg tttacaggat cggggtgcca
     4921 tcgggcatga agcgctcatg gcagcgcaag gtcaattgct gcagttggcg gccgaaagcc
     4981 ccgtgctgac gagtgtgcgc cccaacggtt tggcggacaa tccccgctac aaagtggaca
     5041 ttgattacga aaaggcgcag gcccttggca tagagccgtc cgagatcagt cgacttctgt
     5101 cggtgacctg gggctcgcaa tacgttaacg attttctcca tgaggggcgg gtcaagcgag
     5161 tctatgttca gggtgatgcc cctttccgga tgctgcctga ggattttgag gcgtggtatc
     5221 tgcgagccgc taacggagag atgaccccgt tgagcgaagt gacgaacgga cgctgggagt
     5281 atggctcacc gcgccttgag cgtttcaacg gtgttccgtc ccgtcagatt cagggtgagc
     5341 cggcaccggg ctacagtacc ggtgaggcca tggcggaggt cgaacgtttg atcgctcaac
     5401 tcccggacgg tgtggccggc gcctggagcg gcctgtccta tcaggagcgc caggccggtg
     5461 cccaggcatc cctgctttac gcgctgtcgg cactggtggt gtttctggcg ctcgccgcgc
     5521 tctacgaaag ttggaccata cccatttccg tgatgctggc ggtgcccttg ggcgtactcg
     5581 gggcggtact tgcagcgatg gttcgcgggc ttcctaacga cgtcttcttc caggttggca
     5641 ttctgaccac cgttggtgtg actgcgcgca acgctatact tctggtggag ttcgcccgct
     5701 cgttagagga tcagggaatg aaattgatcg aggcaaccaa ggaagcggcg cgagtgcgct
     5761 tgaggccgat tctgatgaca tcggttgctt tcggcatggg tgtgttgccg cttgctttcg
     5821 ccagtggtgc cggtgcaacc acccgtattg cgataggaac ggcggttctt ggcggcatga
     5881 tttcggcaac gatactggcg acgttcttta ttccactgtt ctatgtggtc gtccgccgta
     5941 tcacggattt tctgagtggc tccagagacc aggatgactc ggctgcggcc gctcccggac
     6001 gggcaaatgg aggtgtggct gatgaccgtt aaggtattgc tgaccacttt gatggtgttg
     6061 tcacttggtg gctgctcact cgcgccgact gttaagaagc ccgagccgtc gatgccggaa
     6121 aactggacgt ccgcacgact ttcggcggac gagaaactcc ctctggactg gtggcgtggc
     6181 tatggcgatc cggcacttgt cacgcttgtt gaggaggcac ttgatgccaa caacgatctg
     6241 cagctcgccg ctgcccgggt tgccgaagca agggcgctcc ttaccggaca gcaggcagaa
     6301 cgatacccac ttctggaggt ggagggctcc gcggtgcgcc agggcccgag tgaggagtcc
     6361 gtcaacagta ctggtggtca acccttcaat gatatccagg ttggcggtgt tctatcgtac
     6421 gagctggatc tctgggggcg gcttgccaat gccagcgaag cagcccgggc acggcttctg
     6481 gccagtgcag cgaatcgcga ggccgtgcga ctggccgtga ttggtgaggt ggccaatggt
     6541 tacttcaatt tgcgtgcgct tgatcgtcag atcgacatcg cggaacgcac cgtgtcgtcc
     6601 cgccgcgaat cggtcgccct gcaacgggcc cggtttgaag gaggcgagat taatgaattg
     6661 gccctgcggc aggcagaatc cgagcttgcg gccgctgaat ccgaacttcc gaggcttcgc
     6721 caacagcgct cccttcagcg aaacgccttg gcagtgcttc tggggcgaga tccacaacgg
     6781 attgtacgta gtgatatttc cgtagcgaat accattggtg agatggatgt tccggccagt
     6841 atccctgcag gccgaccagc agatctgatg gttcggcgac ccgacattgc cgcagccgaa
     6901 caggaattga tcgcggccaa cgccgaaatc ggtgttgccc gggcggcgtt tctgcccagg
     6961 atttcattcg aggggctgct gggcctgcga agtgcgtccg gtggcgactt gttccagggt
     7021 tcggcatcca cgtggcagtt gggcggctcg ttagtcggcc cgttgctgga cttcgggcgt
     7081 tccgaggcac tagtcaatca ggcggaggcg cgtcaacgcc aggccctgat tgcgtatcgt
     7141 caggccattc aggtggcgtt tcgggaggtg ctcgacgcga tggccggggt gcgtggtacc
     7201 gaagatcgcc tggaagcgca ggcccggcag gtagcagcac tgcgaagcac ggctaaactt
     7261 gcgcgattgc gctttgaggg tggcgcgtcc agttacctgg aggtgctgga cgctgaacga
     7321 agcctgttta caaccgaact tgatctcgtg gaaacgcggc gcgatcactt gcagtcgacg
     7381 gttaatttgt atcgcgcgct gggcgggggt tggcagaatt cattgcaatc ttctgattat
     7441 gaaaacactg acggatccaa actcgagtaa ggatctccag gcatcaaata aaacgaaagg
     7501 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     7561 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatacctag ggtacgggtt
     7621 ttgctgcccg caaacgggct gttctggtgt tgctagtttg ttatcagaat cgcagatccg
     7681 gcttcagccg gtttgccggc tgaaagcgct atttcttcca gaattgccat gattttttcc
     7741 ccacgggagg cgtcactggc tcccgtgttg tcggcagctt tgattcgata agcagcatcg
     7801 cctgtttcag gctgtctatg tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa
     7861 tttcatgttc tagttgcttt gttttactgg tttcacctgt tctattaggt gttacatgct
     7921 gttcatctgt tacattgtcg atctgttcat ggtgaacagc tttgaatgca ccaaaaactc
     7981 gtaaaagctc tgatgtatct atctttttta caccgttttc atctgtgcat atggacagtt
     8041 ttccctttga tatgtaacgg tgaacagttg ttctactttt gtttgttagt cttgatgctt
     8101 cactgataga tacaagagcc ataagaacct cagatccttc cgtatttagc cagtatgttc
     8161 tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag atcatactta
     8221 ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt tgcagttaaa
     8281 gcatcgtgta gtgtttttct tagtccgtta tgtaggtagg aatctgatgt aatggttgtt
     8341 ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac gagatccatt
     8401 tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc
     8461 accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa aacccattgg
     8521 ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt aaattcatca
     8581 aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt taataaccac
     8641 tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag attatatttt
     8701 atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac taattctaat
     8761 ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa
     8821 ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc taatacacca
     8881 taagcatttt ccctactgat gttcatcatc tgagcgtatt ggttataagt gaacgatacc
     8941 gtccgttctt tccttgtagg gttttcaatc gtggggttga gtagtgccac acagcataaa
     9001 attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc atttgctttg
     9061 aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc actataccaa
     9121 ttgagatggg ctagtcaatg ataattacta gtccttttcc cgggtgatct gggtatctgt
     9181 aaattctgct agacctttgc tggaaaactt gtaaattctg ctagaccctc tgtaaattcc
     9241 gctagacctt tgtgtgtttt ttttgtttat attcaagtgg ttataattta tagaataaag
     9301 aaagaataaa aaaagataaa aagaatagat cccagccctg tgtataactc actactttag
     9361 tcagttccgc agtattacaa aaggatgtcg caaacgctgt ttgctcctct acaaaacaga
     9421 ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt
     9481 ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc
     9541 tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc ttttatggat
     9601 tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct cagggcgttt
     9661 tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt tcctgccctc
     9721 tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga ctggctaatg
     9781 cacccagtaa ggcagcggta tcatcaacag gcttacccgt cttactgtcc ctagtgcttg
     9841 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     9901 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
     9961 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
    10021 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
    10081 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
    10141 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
    10201 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
    10261 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
    10321 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
    10381 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
    10441 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
    10501 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
    10561 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
    10621 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
    10681 tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccagata
    10741 tc
//
