LOCUS       sequence-173357-        1916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 4, tAOX, Alcohol oxidase 1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Obst U, Lu TK, Sieber V
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion 
            Libraries.
  JOURNAL   ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337.
  PUBMED    28252957
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337."
COMMENT     Sequence Label: pPTK019-4-tAOX1
FEATURES             Location/Qualifiers
     source          1..1916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..247)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     rep_origin      complement(365..953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(445..464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1135..1794
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1201..1220)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1865..1899
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 tctcacttaa tcttctgtac tctgaagagg agtgggaaat accaagaaaa acatcaaact
       61 cgaatgattt tcccaaaccc ctaccacaag atattcatca gctgcgagat aggctgatca
      121 ggagcaagct cgtacgagaa gaaacaaaat gacaaaaaaa atcctatact atataggtta
      181 caaataaaaa agtatcaaaa atgaagcctg catctctcag gcaaatggca ttctgacatc
      241 ctcttgatta ggattgagac cgactacggt tatccacaga atcaggggat aacgcaggaa
      301 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      361 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      421 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      481 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      541 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      601 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      661 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      721 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      781 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      841 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      901 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      961 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1021 tggtcatgat tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca
     1081 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
     1141 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     1201 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     1261 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     1321 cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg
     1381 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     1441 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     1501 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     1561 tttttcgttt cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     1621 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     1681 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     1741 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttgat
     1801 atcgagctcg cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
     1861 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtctggtct cacagc
//
