LOCUS       sequence-173358-        2664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 2, pENO1, Enolase 1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Obst U, Lu TK, Sieber V
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion 
            Libraries.
  JOURNAL   ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337.
  PUBMED    28252957
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337."
COMMENT     Sequence Label: pPTK003-2-pENO1
FEATURES             Location/Qualifiers
     source          1..2664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1016..1050)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1121..1780)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1695..1714
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1962..2550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2451..2470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agaaagcata ctatactatt cgacattcct ttcaatcctg gaattaacag tcacttttaa
       61 aaaagacatc taccgtgaag gtgccgtaga gtatcgcgtt accatatcgc caaaaactga
      121 tatacgccgc ggaaaccagg caaacaattg aaaagaaaaa ttttgaggaa ctctctgcat
      181 cgaagccgtc tagagttacc actagtcaga tgccgcgggc acttgagcac ctcatgcaca
      241 gcaataacac aacacaatgg ttagtagcaa cctgaattcg gtcattgatg catgcatgtg
      301 ccgtgaagcg ggacaaccag aaaagtcgtc tataaatgcc ggcacgtgcg atcatcgtgg
      361 cggggtttta agagtgcata tcacaaattg tcgcattacc gcggaaccgc cagatattca
      421 ttacttgacg caaaagcgtt tgaaataatg acgaaaaaga aggaagaaaa aaaaagaaaa
      481 ataccgcttc taggcgggtt atctactgat ccgagcttcc actaggatag cacccaaaca
      541 cctgcatatt tggacgacct ttacttacac caccaaaaac cactttcgcc tctcccgccc
      601 ctgataacgt ccactaattg agcgattacc tgagcggtcc tcttttgttt gcagcatgag
      661 acttgcatac tgcaaatcgt aagtagcaac ctctcaaggt caaaactgta tggaaacctt
      721 gtcacctcac ttaattctag ctagcctacc ctgcaagtca agagctctcc gtgattccta
      781 gccacctcaa ggtatgcctc tccccggaaa ctgtggcctt ttctggcaca catgatctcc
      841 acgatttcaa catataaata gcttttgata atggcaatat taatcaaatt tattttactt
      901 ctttcttgta acatctctct tgtaatccct tattccttct agctattttt cataaaaaac
      961 caagcaactg cttatcaaca cacaaacact aaatcaaata tgtgagacca gaccaataaa
     1021 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1081 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1141 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1201 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1261 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1321 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
     1381 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1441 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1501 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1561 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1621 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1681 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1741 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1801 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1861 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc
     1921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2101 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2581 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2641 gataaccgta gtcggtctca aacg
//
