LOCUS       sequence-173365-        9900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked 
            alleles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Voutev R, Mann RS
  TITLE     Bxb1 phage recombinase assists genome engineering in Drosophila 
            melanogaster.
  JOURNAL   Biotechniques. 2017 Jan 1;62(1):37-38. doi: 10.2144/000114494.
  PUBMED    28118814
REFERENCE   2  (bases 1 to 9900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2017-01-1- 1"; volume: "62"; issue: "1"; 
            pages: "37-38"
COMMENT     Sequence Label: pRVV653
FEATURES             Location/Qualifiers
     source          1..9900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            665..4801
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(674..692)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1144..1215,1617..1890,1965..2619,2681..2996,3217..3348,
                     3419..4033)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4861..4908
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    6755..6802
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     intron          7065..7130
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7260..7280
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7552..7686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7589..7608)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7643..7662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(7712..7730)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7751..7767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7751..7767)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7764..7786)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7775..7791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7799..7805,7806..7823,7824..7829))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7844..7865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7982..7999)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8153..8741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8233..8252)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8912..9703,9704..9772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9535..9554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9773..9877)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac
      721 attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt taaatttact
      781 tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc aaaatgacat
      841 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
      901 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc
      961 cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
     1021 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
     1081 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
     1141 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
     1201 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
     1261 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
     1321 aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat tattctggta gctgttctcg
     1381 ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc attcattttc
     1441 gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
     1501 acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta ctttttgttc
     1561 ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg
     1621 acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg
     1681 gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc caactagccg
     1741 agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct
     1801 ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac
     1861 cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg
     1921 tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta
     1981 tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa
     2041 tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct
     2101 caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga
     2161 tgacctcttt atcggctccc taacggccag ggaacacctg attttccagg ccatggtgcg
     2221 gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga
     2281 gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct
     2341 gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gcactaaccg atccgccgct
     2401 tctgatctgc gatgagccca cctccggact ggactcattt accgcccaca gcgtcgtcca
     2461 ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc
     2521 ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt
     2581 cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta
     2641 agggtatcta gcattacatt acatctcaac tcctatccag cgtgggtgcc cagtgtccta
     2701 ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga
     2761 tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgctattagc aaagtagccc
     2821 gggatatgga gcagttgttg gccaccaaaa atttggagaa gccactggag cagccggaga
     2881 atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct
     2941 ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga
     3001 gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga
     3061 ttttagttag aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt
     3121 ttatagtttt gcagaaaata aataaatttc atttaactcg cgaacatgtt gaagatatga
     3181 atattaatga gatgcgagta acattttaat ttgcagatgg ttgccatctt gattggcctc
     3241 atctttttgg gccaacaact cacgcaagtg ggcgtgatga atatcaacgg agccatcttc
     3301 ctcttcctga ccaacatgac ctttcaaaac gtctttgcca cgataaatgt aagtcttgtt
     3361 tagaatacat ttgcatatta ataatttact aactttctaa tgaatcgatt cgatttaggt
     3421 gttcacctca gagctgccag tttttatgag ggaggcccga agtcgacttt atcgctgtga
     3481 cacatacttt ctgggcaaaa cgattgccga attaccgctt tttctcacag tgccactggt
     3541 cttcacggcg attgcctatc cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa
     3601 ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg tccttcggat atctaatatc
     3661 ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt ccgccggtta tcataccatt
     3721 cctgctcttt ggcggcttct tcttgaactc gggctcggtg ccagtatacc tcaaatggtt
     3781 gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc aatgggcgga
     3841 cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa
     3901 ggtcatcctg gagacgctta acttctccgc cgccgatctg ccgctggact acgtgggtct
     3961 ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac ttcgggcccg
     4021 acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt ttaccattat
     4081 taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca
     4141 ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt
     4201 agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa
     4261 ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg
     4321 cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc
     4381 tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt
     4441 tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg tctcaataga
     4501 aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg tatatatttc
     4561 cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat tttttgtgac
     4621 ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg tttgttcctt
     4681 gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata tatcaaaaat
     4741 aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc actggaacta
     4801 ggctagattt aaatgcatgc cctgcaggaa gcttcctagg aggcctagat ctgcggccgc
     4861 ggtttgtacc gtacaccact gagaccgcgg tggttgacca gacaaacctt aattaagaag
     4921 ttcctattct tcaaaaggta taggaacttc ccgcgagctc gcccggggat ctaattcaat
     4981 tagagactaa ttcaattaga gctaattcaa ttaggatcca agcttatcga tttcgaaccc
     5041 tcgaccgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca
     5101 agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag
     5161 ctaaacaatc gggcggccgc actagagccg gtcgccacca tgaggtcttc caagaatgtt
     5221 atcaaggagt tcatgaggtt taaggttcgc atggaaggaa cggtcaatgg gcacgagttt
     5281 gaaatagaag gcgaaggaga ggggaggcca tacgaaggcc acaataccgt aaagcttaag
     5341 gtaaccaagg ggggaccttt gccatttgct tgggatattt tgtcaccaca atttcagtat
     5401 ggaagcaagg tatatgtcaa gcaccctgcc gacataccag actataaaaa gctgtcattt
     5461 cctgaaggat ttaaatggga aagggtcatg aactttgaag acggtggcgt cgttactgta
     5521 acccaggatt ccagtttgca ggatggctgt ttcatctaca aggtcaagtt cattggcgtg
     5581 aactttcctt ccgatggacc tgttatgcaa aagaagacaa tgggctggga agccagcact
     5641 gagcgtttgt atcctcgtga tggcgtgttg aaaggagaga ttcataaggc tctgaagctg
     5701 aaagacggtg gtcattacct agttgaattc aaaagtattt acatggcaaa gaagcctgtg
     5761 cagctaccag ggtactacta tgttgactcc aaactggata taacaagcca caacgaagac
     5821 tatacaatcg ttgagcagta tgaaagaacc gagggacgcc accatctgtt cctttagcgg
     5881 ccatcgaatt cgagctcgcc cactaagcgt cgcgccactt caacgctcga tgggagcgtc
     5941 attggtgggc ggggtaaccg tcgaaatcag tgtttacgct tccaatcgca acaaaaaatt
     6001 cactgcaaca ctgaaaagca tacgaaaacg atgaagattg tacgagaaac cataaagtat
     6061 tttatccaca aagacacgta tagcagaaaa gccaagttaa ctcggcgata agttgtgtac
     6121 acaagaataa aatcggccag attcagtgtt gtcagaaata agaaaacccc actatgtttt
     6181 tctttgcctt ttctttctcc cagcgatcat tcatttcgtg gtgaaagaac ggggtcattg
     6241 cacggagttt cgactgcggg aaagcagagc tgccgttcac ttcgtctata attagcgctt
     6301 tctattttcc ccgattcggg ccgctgctgc gcttttccgc ctgctgtttg tggcaagtgt
     6361 agcagcaggc tgtgcacgca gtgtggcatg cacttggctt tccaccgttg gtatcgattc
     6421 tctgggacga tgagtcattc ctttcggggc cacagcataa tcgttgccag ctcaccgaaa
     6481 tggtgacttc atttcttaac tgccgtcaag catgcgattg tacatacata catatttata
     6541 tatgtacata tttatgtgac tatggtaggt cgatataata gcaatcaacg caagcaaatg
     6601 tgtcagtcct gcttacagga acgattctat ttagtaattt tcgttgtata aagtaattat
     6661 gtatgtatgt aagccccata aatctgaaac aattaggcaa aaccatgcga agctgaagtt
     6721 cctattcttc aaaaggtata ggaacttcac cggtggtttg tctggtcaac caccgcggtc
     6781 tcagtggtgt acggtacaaa ccggcgcgcc gctagccata tggggattgg tggcgacgac
     6841 tcctggagcc cgtcagtatc ggcggaatta cagctgagcg ccggtcgcta ccattaccag
     6901 ttggtctggt gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta
     6961 aggaaatccc tcgagggtac ctctagagga tctttgtgaa ggaaccttac ttctgtggtg
     7021 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     7081 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     7141 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     7201 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     7261 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     7321 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     7381 aaaaagctgc actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga
     7441 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     7501 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     7561 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7621 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7681 tggatccact agttctagcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg
     7741 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7801 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7861 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7921 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7981 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8041 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8101 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8161 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8221 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8281 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8341 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8401 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8461 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8521 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8581 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8641 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8701 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8761 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8821 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8881 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8941 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9001 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9061 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9121 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9181 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9241 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9301 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9361 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9421 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9481 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9541 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9601 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9661 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9721 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9781 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9841 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
