LOCUS       sequence-173371-        1914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 3a, Killer followed by alpha-MF-Delta - secretion signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Obst U, Lu TK, Sieber V
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion 
            Libraries.
  JOURNAL   ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337.
  PUBMED    28252957
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337."
COMMENT     Sequence Label: pPTK014-3a-Killer-alpha-MF-Delta
FEATURES             Location/Qualifiers
     source          1..1914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(323..357)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(428..1087)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1002..1021
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1269..1857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1758..1777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgttctttc ctgcgttatc ccctgattct gtggataacc gtagtcggtc tcatatgacc
       61 aaaccaacgc aagtcttagt tcgttcagtc tctattttat tcttcatcac actgttgcac
      121 ttggttgttg caatgagatt tcctagtatt ttcactgctg tgctatttgc cgctagttcc
      181 gctctagctg ctccagttaa tactactact gaagatgaat tggagggtga cttcgatgtt
      241 gctgttctgc ctttttccgc ttctatcgca gccaaggaag aaggtgtatc tctagagaag
      301 cgtggttctt gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
      361 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
      421 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
      481 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
      541 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
      601 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa
      661 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
      721 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
      781 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
      841 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
      901 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
      961 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1021 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1081 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     1141 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc
     1201 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1261 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1321 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1381 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1441 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1501 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1561 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1621 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1681 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1741 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1801 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1861 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcac
//
