LOCUS       sequence-173372-        2250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 2, pTPI1, Triose phosphate isomerase 1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Obst U, Lu TK, Sieber V
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion 
            Libraries.
  JOURNAL   ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337.
  PUBMED    28252957
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337."
COMMENT     Sequence Label: pPTK004-2-pTPI1
FEATURES             Location/Qualifiers
     source          1..2250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        65..584
                     /gene="S. cerevisiae TPI1"
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose 
                     phosphate isomerase"
     terminator      complement(602..636)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(707..1366)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1281..1300
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1548..2136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2037..2056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgtttaaag attacggata tttaacttac ttagaataat gccatttttt tgagttataa
       61 taatcctacg ttagtgtgag cgggatttaa actgtgagga ccttaataca ttcagacact
      121 tctgcggtat caccctactt attcccttcg agattatatc taggaaccca tcaggttggt
      181 ggaagattac ccgttctaag acttttcagc ttcctctatt gatgttacac ctggacaccc
      241 cttttctggc atccagtttt taatcttcag tggcatgtga gattctccga aattaactaa
      301 agcaatcaca caattctctc ggataccacc tcggttgaaa ctgacaggtg gtttgttacg
      361 catgctaatg caaaggagcc tatatacctt tggctcggct gctgtaacag ggaatataaa
      421 gggcagcata atttaggagt ttagtgaact tgcaacattt actattttcc cttcttacgt
      481 aaatattttt ctttttaatt ctaaatcaat ctttttcaat tttttgtttg tattcttttc
      541 ttgcttaaat ctataactac aaaaaacaca tacataaact aaaatatgtg agaccagacc
      601 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      661 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
      721 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
      781 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
      841 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
      901 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
      961 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1021 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1081 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     1141 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1201 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1261 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     1321 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     1381 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     1441 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt
     1501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1681 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2161 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2221 tctgtggata accgtagtcg gtctcaaacg
//
