LOCUS       sequence-173377-        1893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 3a, alpha-mating factor no Kex recognition site - secretion 
            signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Obst U, Lu TK, Sieber V
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion 
            Libraries.
  JOURNAL   ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337.
  PUBMED    28252957
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337."
COMMENT     Sequence Label: pPTK008-3a-alpha-MF-Delta no Kex
FEATURES             Location/Qualifiers
     source          1..1893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(248..282)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(353..1012)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     927..946
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1194..1782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1683..1702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgagattcc catcaatttt tactgctgtt ctgttcgccg cttctagtgc acttgccatg
       61 agatttccta gtattttcac tgctgtgcta tttgccgcta gttccgctct agctgctcca
      121 gttaatacta ctactgaaga tgaattggag ggtgacttcg atgttgctgt tctgcctttt
      181 tccgcttcta tcacagccaa ggaagaaggt gtatctctag agaagcgtgg ttcttgagac
      241 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
      301 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
      361 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
      421 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      481 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
      541 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
      601 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
      661 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
      721 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
      781 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
      841 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
      901 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
      961 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1021 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1081 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
     1141 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1201 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1261 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1321 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1381 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1441 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1501 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1561 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1621 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1681 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1741 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1801 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1861 cctgattctg tggataaccg tagtcggtct cat
//
