LOCUS       sequence-173463-        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orange color variant of bright luminescent protein, fused to actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, 
            Nagai T
  TITLE     Five colour variants of bright luminescent protein for real-time 
            multicolour bioimaging.
  JOURNAL   Nat Commun. 2016 Dec 14;7:13718. doi: 10.1038/ncomms13718.
  PUBMED    27966527
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-14"; volume: "7:13718. doi"; pages: " 
            10.1038/ncomms13718"
COMMENT     Sequence Label: OeNL-Actin_pcDNA3
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             241..891
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             241..891
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg
       61 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
      121 actccgatcc aaaggattgt cctgagcggt ctcgagggcg gtagcggtgg cagcgtgagc
      181 gtgatcaagc ccgagatgaa gaaggtggag gacgccgtgg cccactccac tctcgagggc
      241 gtgagcgtga tcaagcccga gatgaagatg aggtactaca tggacggctc cgtcaatggg
      301 catgagttca caatcgaggg tgagggcaca ggcagacctt acgagggaca tcaggagatg
      361 acactgcgcg tcacaatggc cgagggcggg ccaatgcctt tcgccttcga cctggtgtcc
      421 cacgtgttct gttacggcca cagagttttt actaaatatc cagaagagat cccagactat
      481 ttcaagcagg cctttcctga gggcctgtcc tgggagaggt ccctggagtt cgaggacggc
      541 ggctccgcct ccgtgagcgc ccacatcagc ctgaggggca acaccttcta ccacaagtcc
      601 aagttcaccg gcgtgaactt ccccgccgac ggccccatca tgcagaacca gagcgtggac
      661 tgggagccct ccaccgagaa gatcaccgcc agcgacggcg tgctgaaggg cgacgtgacc
      721 atgtacctga agctggaggg cggcggcaac cacaagtgcc agatgaagac cacctacaag
      781 gccgccaagg agatcctgga gatgcccggc gaccactaca tcggccacag gctggtgagg
      841 aagaccgagg gcaacatcac cgagcaggtg gaggacgccg tggcccactc cgagctcgaa
      901 aatgggctga agatcgacat ccatgtcatc atcccgtatg aaggtctgag cggcgaccaa
      961 atgggccaga tcgaaaaaat ttttaaggtg gtgtaccctg tggatgatca tcactttaag
     1021 gtgatcctgc actatggcac actggtaatc gacggggtta cgccgaacat gatcgactat
     1081 ttcggacggc cgtatgaagg catcgccgtg ttcgacggca aaaagatcac tgtaacaggg
     1141 accctgtgga acggcaacaa aattatcgac gagcgcctga tcaaccccga cggctccctg
     1201 ctgttccgag taaccatcaa cggagtgacc ggctggcggc tgtgcgaacg cattctggcg
//
