LOCUS       sequence-17349-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, 
            Lawrence HJ, Cleary ML, Largman C
  TITLE     Hox homeodomain proteins exhibit selective complex stabilities with 
            Pbx and DNA.
  JOURNAL   Nucleic Acids Res 1996 Mar 1;24(5):898-906.
  PUBMED    8600458
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "1996-03-1"; volume: "24(5)"; pages: "898-906"
COMMENT     Sequence Label: HOXB1 (human) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             93..125
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 aatattttgt ttactttaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggtgccg cgcggcagcc atatggctag catgactggt ggacagcaaa
      121 tgggtcgcgg atccatggac tataatagga tgaactcctt cttagagtac ccactctgta
      181 accggggacc cagcgcctac agcgcccaca gcgccccaac ctcctttccc ccaagctcgg
      241 ctcaggcggt tgacagctat gcaagcgagg gccgctacgg tggggggctg tccagccctg
      301 cgtttcagca gaactccggc tatcccgccc agcagccgcc ttcgaccctg ggggtgccct
      361 tccccagctc cgcgccctcg gggtatgctc ctgccgcctg cagccccagc tacgggcctt
      421 ctcagtacta ccctctgggt caatcagaag gagacggagg ctattttcat ccctcgagct
      481 acggggccca gctagggggc ttgtccgatg gctacggagc aggtggagcc ggtccggggc
      541 catatcctcc gcagcatccc ccttatggga acgagcagac cgcgagcttt gcaccggcct
      601 atgctgatct cctctccgag gacaaggaaa caccctgccc ttcagaacct aacaccccca
      661 cggcccggac cttcgactgg atgaaggtta agagaaaccc acccaagaca gcgaaggtgt
      721 cagagccagg cctgggctcg cccagtggcc tccgcaccaa cttcaccaca aggcagctga
      781 cagaactgga aaaggagttc catttcaaca agtacctgag ccgggcccgg agggtggaga
      841 ttgccgccac cctggagctc aatgaac
//
