LOCUS       sequence-17350-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, 
            Lawrence HJ, Cleary ML, Largman C
  TITLE     Hox homeodomain proteins exhibit selective complex stabilities with 
            Pbx and DNA.
  JOURNAL   Nucleic Acids Res 1996 Mar 1;24(5):898-906.
  PUBMED    8600458
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "1996-03-1"; volume: "24(5)"; pages: "898-906"
COMMENT     Sequence Label: HOXB2 (human) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             85..102
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             106..138
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 aattccntct gaatattttg tttaacttta agaaggagat ataccatggg cagcagccat
       61 cattatcatc atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcatgact
      121 ggtggacagc aaatgggtcg cggatccaat tttgaatttg agagggagat tgggtttata
      181 aacagccagc cgtcgctcgc cgagtgtctg acttccttcc ccgctgtctt ggagacattt
      241 caaacttcat caatcaagga gtcgacatta attcctcctc ctcctccttt cgagcaaacc
      301 ttccccagcc tccagcccgg cgcctccacc cttcagagac ccaggagcca aaagcgagcc
      361 gaagatgggc ctgctctgcc gccgccaccg ccgccgccac tccccgctgc ccccccggcc
      421 cccgagttcc cttggatgaa agagaagaaa tccgccaaga aacccagcca atccgccacg
      481 tctccttctc cggccgcctc cgccgttccg gcctccgggg tcggatcgcc tgcagatggc
      541 ctgggactgc cggaggctgg tggcggcggg gcgcgcaggc tgcgcanggc ttacaccaac
      601 acgcagctgc tggaactgga gaaggaatcn ttttatnata aagtactngt gcnggccacg
      661 cccccttcga gatcgcggcc tt
//
