LOCUS       sequence-173512-        7750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid 2 to prepare Penn State DNA molecular 
            weight ladders.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Henrici RC, Pecen TJ, Johnston JL, Tan S
  TITLE     The pPSU Plasmids for Generating DNA Molecular Weight Markers.
  JOURNAL   Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1.
  PUBMED    28550309
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-05-26"; volume: "7(1):2438. doi"; pages: " 
            10.1038/s41598-017-02693-1"
COMMENT     Sequence Label: pPSU2
FEATURES             Location/Qualifiers
     source          1..7750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5354..5371)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5354..5370)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5363..5385)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5579..5598)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5698..5720
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5758..5776)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5844..5948
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5949..6017,6018..6809)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6167..6186)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6980..7568
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7469..7488
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7722..7739
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttga
      241 tatcatcaaa gccatgaaca aagcagccgc gctggatgaa ctgataccgg ggttgctgag
      301 tgaatatatc gaacagtcag gttaacctgc agcggcattt tgtccgcgcc gggcttcgct
      361 cactgttcag gccggagcca cagaccgccg ttgaatgggc ggatgctaat tactatctcc
      421 cgaaagaatc cgcataccag gaagggcgct gggaaacact gccctttcag cgggccatca
      481 tgaatgcgat gggcagcgac tacatccgtg aggtgaatgt ggtgaagtct gcccgtgtcg
      541 gttattccaa aatgctgctg ggtgtttatg cctactttat agagcataag cagcgcaaca
      601 cccttatctg gttgccaacg gatggtgatg ccgagaactt tatgaaaacc cacgttgagc
      661 cgactattcg tgatattccg tcgctgctgg cgctggcccc gtggtatggc aaaaagcacc
      721 gggataacac gctcaccatg aagcgtttca ctaatgggcg tggcttctgg tgcctgggcg
      781 gtaaagcggc aaaaaactac cgtgaaaagt cggtggatgt ggcgggttat gatgaacttg
      841 ctgcttttga tgatgatatt gaacaggaag gctctccgac gttcctgggt gacaagcgta
      901 ttgaaggctc ggtctggcca aagtccatcc gtggctccac gccaaaagtg agaggcacct
      961 gtcagattga gcgtgcagcc agtgaagcga tatcggatct ttccgagcat ttattaagca
     1021 tttcgctata agttctcgct ggaagaggta gttttttcat tgtactttac cttcatctct
     1081 gttcattatc atcgctttta aaacggttcg accttctaat cctatctgac cattataatt
     1141 ttttagaatg gtttcataag aaagctctga atcaacggac tgcgataata agtggtggta
     1201 tccagaattt gtcacttcaa gtaaaaacac ctcacgagtt aaaacaccta agttctcacc
     1261 gaatgtctca atatccggac ggataatatt tattgcttct cttgaccgta ggactttcca
     1321 catgcaggat tttggaacct cttgcagtac tactggggaa tgagttgcaa ttattgctac
     1381 accattgcgt gcatcgagta agtcgcttaa tgttcgtaaa aaagcagaga gcaaaggtgg
     1441 atgcagatga acctctggtt catcgaataa aactaatgac ttttcgccaa cgacatctac
     1501 taatcttgtg atagtaaata aaacaattgc atgtccagag ctcattcgaa gcagatattt
     1561 ctggatattg tcataaaaca atttagtgaa tttatcatcg tccacttgaa tctgtggttc
     1621 attacgtctt aactcttcat atttagaaat gaggctgatg agttccatat ttgaaaagtt
     1681 ttcatcacta cttagttttt tgatagcttc aagccagagt tgtctttttc tatctactct
     1741 catacaacca ataaatgctg aaatgaattc taagcggaga tcgcctagtg attttaaact
     1801 attgctggca gcattcttga gtccaactgc agagtattgt gtaccttttg ctgggtcagg
     1861 ttgttcttta gccggcctgc aggaagagga tggtgcagca accaacaaga aaacactggc
     1921 agattacgcc cgtgccttat ccggagagga tgaatgacgc gacaggaaga acttgcctgc
     1981 agtaactttc cccacaacgg aacaactctc attgcatggg atcattgggt actgtgggtt
     2041 tagtggttgt aaaaacacct gaccgctatc cctgatcagt ttcttgaagg taaactcatc
     2101 acccccaagt ctggctatgc agaaatcacc tggctcaaca gcctgctcag ggtcaacgag
     2161 aattaacatt ccgtcactgc agacggctca ggatacggat aacggctact ccgtgtttga
     2221 gcagtcactg ctgcggtata tcgctgccgg gctgggtgtc tcgtatgagc agctttcccg
     2281 gaattacgcc cagatgagct actccacggc acgggccagt gcgaacgagt cgtgggcgta
     2341 ctttatgggg cggcgaaaat tcgtcgcatc ccgtcaggcg agccagatgt ttctgtgctg
     2401 gctggaagag gccatcgttc gccgcgtggt gacgttacct tcaaaagcgc gcttcagttt
     2461 tcaggaagcc agatctctgc aggttgacgg ttttccacca tcgcacgccg ggaccatcac
     2521 cgtgtatgaa gattcacaac cggggacgct gaatgatttt ctctgtgcca tgacggagga
     2581 tgatgcccgg ccggaggtgc tgcgtcgtct tgaactgatg gtggaagagg tggcgcgtaa
     2641 cgcgtccgtg gtggcacaga gtacggcaga cgcgaagaaa tcagccggcg atgccagtgc
     2701 atcagctgct caggtcgcgg cccttgtgac tgatgcaact gactcagcac gcgccgccag
     2761 cacgtccgcc ggacaggctg catcgtcagc tcaggaagcg tcctccggcg cagaagcggc
     2821 atcagcaaag gccactgaag cggaaaaaag tgccgcagcc gcagagtcct caaaaactgc
     2881 aggcggatta caacacgctg atggcggcgg cgaaaaagga ttatgaagcg acgctgaaaa
     2941 agccgaaaca gtccagcgtg aaggtgtctg cgggcgatcg tcaggaagac agtgctcatg
     3001 ctgccctgct gacgcttcag gcagaactcc ggacgctgga gaagcatgtc ggagcaaatg
     3061 agaaaatcag ccagcagcgc cgggatttgt ggaaggcgga gagtcagttc gcggtactgg
     3121 aggaggcggc gcaacgtcgc cagctgtctg cacaggagaa atccctgctg gcgcataaag
     3181 atgagacgct ggagtacaaa cgccagctgg ctgcacttgg cgacaaggtt acgtatcagg
     3241 agcgcctgaa cgcgctggcg cagcaggcgg ataaattcgc acagcagcaa cgggcaaaac
     3301 gggccgccat tgatgcgaaa agccgggggc tgactgaccg gcaggcagaa cgggaagcca
     3361 cggaacagcg cctgaactgc agggatccct tctggggccg gacggcatga catcgctgcg
     3421 cgaatatgcc ggttatcacg gcggtggcag cggatttgga gggcagttgc ggtcgtggaa
     3481 cccaccgagt gaaagtgtgg atgcagccct gttgcccaac tttacccgtg gcaatgcccg
     3541 cgcagacgat ctggtacgca ataacggcta tgccgccaac gccatccagc tgcatcagga
     3601 tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat
     3661 cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc
     3721 cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg
     3781 ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac
     3841 cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa
     3901 cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg
     3961 tgcggcgctg ggacatctgc agtgtacaga tatccaaatg aagccatagg catttgttat
     4021 tttggctctg tcagctgcat aacgccaaaa aatatattta tctgcttgat cttcaaatgt
     4081 tgtattgatt aaatcaattg gatggaattg tttatcataa aaaattaatg tttgaatgtg
     4141 ataaccgtcc tttaaaaaag tcgtttctgc aagcttggct gtatagtcaa ctaactcttc
     4201 tgtcgaagtg atatttttag gcttatctac cagttttaga cgctctttaa tatcttcagg
     4261 aattatttta ttgtcatatt gtatcatgct aaatgacaat ttgcttatgg agtaatcttt
     4321 taattttaaa taagttattc tcctggcttc atcaaataaa gagtcgaatg atgttggcga
     4381 aatcacatcg tcacccattg gattgtttat ttgtatgcca agagagttac agcagttata
     4441 cattctgcca tagattatag ctaaggcatg taataattcg taatctttta gcgtattagc
     4501 gacccatcgt ctttctgatt taataataga tgattcagtt aaatatgaag gtaatttctt
     4561 ttgtgcaagt ctgactaact tttttatacc aatgtttaac atactttcat ttgtaataaa
     4621 ctcaatgtca ttttcttcaa tgtaagatga aataagagta gcctttgcct cgctatacat
     4681 ttctaaatcg ccttgttttt ctatcgtatt gcgagaattt ttagcccaag ccattaatgg
     4741 atcatttttc catttttcaa taacattatt gttataccaa atgtcatatc ctataatctg
     4801 gtttttgttt ttttgaataa taaatgttac tgttcttgcg gtttggagga attgattcaa
     4861 attcaagcga aataattcag ggtcaaaata tgtatcaatg cagcatttga gcaagtgcga
     4921 taaatcttta agtcttcttt cccatggttt tttagtcata aaactctcca ttttgatagg
     4981 ttgcatgcta gatgctgata tattttagag gtgataaaat taactgctta actgtcaatg
     5041 taatacaagt tgtttgatct ttgcaatgat tcttatcaga aaccatatag taaattagtt
     5101 acacaggaaa tttttaatat tattattatc attcattatg tattaaaatt agagttgtgg
     5161 cttggctctg ctaacacgtt gctcatagga gatatggtag agccgcagac acgtcgtatg
     5221 caggaacgtg ctgtggctgg ctggtgaact tccgatagtg cgggtgttga atgatttcca
     5281 gttgctaccg attttacata ttttttgcat gagagaattt gtaccacctc ccaccgacca
     5341 tctatgagaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     5401 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     5461 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     5521 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     5581 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     5641 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5701 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5761 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5821 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5881 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5941 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6001 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6061 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6121 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6181 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6241 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6301 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6361 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6421 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6481 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6541 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6601 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6661 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6721 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6781 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6841 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6901 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6961 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7021 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7081 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7141 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7201 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7261 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7321 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7381 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7441 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7501 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7561 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7621 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7681 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7741 aagcggaaga
//
