LOCUS       sequence-173516-        7634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PI(DM): Photo-activatable/light sensitive.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, 
            Dokholyan NV, Hahn KM
  TITLE     Engineering extrinsic disorder to control protein activity in living
            cells.
  JOURNAL   Science. 2016 Dec 16;354(6318):1441-1444.
  PUBMED    27980211
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-12-16"; volume: "354(6318)"; pages: "1441-1444"
COMMENT     Sequence Label: mCherry-PA(WT)-Vav2
FEATURES             Location/Qualifiers
     source          1..7634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        133..324
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     281..301
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(326..430)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      457..912
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(544..563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     754..775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    919..1040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(930..949)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     984..1003
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3630..4058)
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     CDS             complement(4155..4862)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4430..4449)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4699..4717
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(4894..5097)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4923..4943)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5098..5401
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5576..6164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5656..5675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6493..6540
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6565..6584
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6772..7566)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6772..7566)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6883..6902)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7493..7512
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(7601..7634,1..324))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(7615..7634,1..116)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
       61 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
      121 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
      181 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
      241 tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
      301 cacagctggt tctttccgcc tcaggactct tcctttttca atattattga agcatttatc
      361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
      421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
      481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      661 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      901 cgcttacaat ttacgcctta agatacattg atgagtttgg acaaaccaca actagaatgc
      961 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     1021 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     1081 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     1141 atgatcagtt atctagaacg ggtcctgtcc aggtctcgtc ctcactggac gccttcctct
     1201 tccacgtacg tcgaaggaaa ccaaccgatc cgcccatttg tctctccttt ccaccagccc
     1261 tggtccccac caattcggct gtagatcttc accacatcac cttctcgcag tgacagctcc
     1321 cgcatgtctc gagcagcaaa gttgtacctg gccacggctg tgccgatgac ccgcggtgtg
     1381 aacactggag accggcttga ggccctgctg gtggttcgct cccgagactt gtaggggaac
     1441 ttgagtgtag tgtccaactg cttgaagctt tccttgagtg agtgacactg gtagtactcc
     1501 accagctcca ggaggctctc aaacttctta gcttccgtga tgtggatcca gctatctttc
     1561 tccaccacct tgatatgttt cacctcatca ttgaacttga tgctgatggc gaagcgttct
     1621 gcctctgctg gacgctctct gatgaggtag gtcccactgg catgagattt gagcagattg
     1681 tccgtctgct gccgttccat gttgccagca aaccacgggt aggcagtgta atcgatctcc
     1741 cgggatggtg ggcggccagt aggcggcctt ccatccactg ggcagggctt cacagatgag
     1801 ctgggaaaat accctgactt cctggtttga accagccgcc cttcccacca aggagaatcg
     1861 gggtcacccc ggagcagttc tatcacgtct cctgtctgga aggtcaacac aggcttccca
     1921 ggaggggctg ggttaccatg gtaattctgc acagcaacca tcttgggacc tggtcctgct
     1981 ccaggagcat ccacatctgc aggtgaactc atcttgcagg gggggatcac ctccaggcat
     2041 tccttgtgtg ccccgacgcc acatctggta cacaggtatc cctggtagaa ggtacccctg
     2101 agaaacatct tgcaggcttt gcagttggta gtcttgtcga atgtgtacat ctggaagcta
     2161 tgatggttgg cattggcctt atctggcttg atgtttgaca tggccatctc gaactgctcc
     2221 atccacttcc gcttcatgtc ttccgtcttg cagaagaact gaaagccttg ctttccttgg
     2281 aggtgaatca ggtagaagcc ataggaccac ttcttgatgt ccttgttgtg catcgggtca
     2341 tcggtcatct tgtggaagag cagctcaatg acctccttca gctcatagct gtagcccttc
     2401 ctcttgcaca cgatgaccac cttgtcaaat aggaacaggt acctgtcttg cttggtgtgg
     2461 ttgactatgg accggacttt aagctccccg tcaatctttg gcctcccaaa ttcctccagc
     2521 ttcacttgca ggttttctat ggagcactgg aactcgctaa tcttcttcaa ggtctccttg
     2581 tcccgcttca cttcattgat gtacatggcc aagtcctgca tggcttccag ggcttctttg
     2641 agctgttgtc tttctggtcg gtctgcagaa tggctcagga gctccttgag cagcaggtgg
     2701 tacttcagca cccgttgcat gggcaccacc agcaggtctt gcagcttgaa cttgccatcc
     2761 tgaaccctga gtgtgcactc ctccactttc tgcctgaagt cctctcggct ggcgaggagc
     2821 tggttcagtg tactctgagc gtgttccatg tggctacagt actctccata gatcaggagc
     2881 ctttccttaa actccagaaa gaccttagcc agggtactgc caccagccat catggacaca
     2941 tcgatggctc gcagaaagct gtgatgcacc ttgatgaggt cctccaggtt gatgaagaca
     3001 gcagccatat ccgccgggct cagcaccagc cgcaagggac ccatgtagtt cttctcaatg
     3061 tcctccaggg tgcggtagta cttggcctcg gtctcctgaa tctctaacaa gcagcagctt
     3121 ctcttgtcgt cctcagtcat gcccattttc atgggctgct gcacctccac cttgatgatg
     3181 tcctcataaa tgtcatctcc ttcatcttca cacgggacac agtcgtagat gtcctcaccc
     3241 aggtcatgct cgtcagccag ctcttccagg ctccggtaga cgtcgtcatc attttcagct
     3301 gtctcctctg atggaaatgg cctgatccct ttgctctgtg cgatgctgtg cagggacaga
     3361 cgggacactg cagagatgac cttcccgaag tctcggacat caaagaggtc gaaagggtca
     3421 aacagctcac tgtttcttag tccaaatttg tcatggcaga ctttgaggaa ggtgcgaata
     3481 ttcttcagac acaagaactg ggacatctgc ggccggaagt tgatgtcctt aaggtcgatg
     3541 gaaccggggg agaggttgtg cagcagctgg cacagaagga cgccgtcgcg cagcgcctgc
     3601 gccaggtcga agaccaccgc accactgcca agttcttttg ccgcctcatc aatattttct
     3661 gcagttttct taatcagcat gactccctct ctctcggcag catctcggac atgctcagtt
     3721 ccatccaact gaaccccaat aaagtactgg acatctccct tctgatctcg cataggctgc
     3781 aagtgaaaga ggttccagaa ctttttacca ctctttgtat aattaatcag ctgaacagtg
     3841 acctctgttt ggttatctat ggcatctcta atttttctca ctgtcgcgcg atcagtttca
     3901 ggaccttgta gaaacctgca gtttcttccc aaaatttctt cacggctata ttctgtcaac
     3961 tgcaagaaac tatcggacgc gaatataatg ggattatctg gcaatcttgg gtcagtaatg
     4021 acaaagttct tctcaatacg ttcaagtgta gtagccaaac ctgatcccga gggccacacg
     4081 acgcggtggt tgggcggcag gaccttgcag tcgatgagcc agcggccgca ttgccgccac
     4141 tgctccattc cggacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
     4201 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg
     4261 ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc
     4321 tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg
     4381 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc
     4441 attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg
     4501 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
     4561 acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg
     4621 gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
     4681 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
     4741 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
     4801 accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc
     4861 atggtgctag cggatctgac ggttcactaa accagctctg cttatataga cctcccaccg
     4921 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     4981 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     5041 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     5101 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     5161 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     5221 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     5281 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     5341 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     5401 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     5461 aatgcatggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5521 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5581 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5641 actcgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5701 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5761 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5821 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5881 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5941 ggattagcag agcgaggtat gtaggcggtg atacagagtt cttgaagtgg tggcctaact
     6001 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6061 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6121 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6181 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6241 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6301 tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg
     6361 cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg
     6421 cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa
     6481 ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg
     6541 tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag
     6601 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
     6661 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg
     6721 atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa
     6781 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     6841 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     6901 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     6961 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     7021 ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     7081 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     7141 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     7201 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     7261 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     7321 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     7381 gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     7441 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     7501 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     7561 aatcatgcga aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc
     7621 caaaaaagcc tcct
//
