LOCUS       sequence-173523-        9542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tetracyline-inducible lentiviral vector with no insert for use in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Chowdhury B, Porter EG, Stewart JC, Ferreira CR, Schipma MJ, 
            Dykhuizen EC
  TITLE     PBRM1 Regulates the Expression of Genes Involved in Metabolism and 
            Cell Adhesion in Renal Clear Cell Carcinoma.
  JOURNAL   PLoS One. 2016 Apr 21;11(4):e0153718. doi: 
            10.1371/journal.pone.0153718. eCollection 2016.
  PUBMED    27100670
REFERENCE   2  (bases 1 to 9542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-21"; volume: "11(4):e0153718. doi"; pages: " 
            10.1371/journal.pone.0153718. eCollection 2016"
COMMENT     Sequence Label: TetO-FUW-pgk-puro
FEATURES             Location/Qualifiers
     source          1..9542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        42..541
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(77..100)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     474..493
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             562..1161
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(562..581)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1058..1078
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1185..1773
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1238..1258)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1656..1667)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1776..1792)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1777..1793)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2298..2478
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2504..2521)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2510..2734
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2780..3208
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2867..2886)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3077..3098
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3217..3237)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3222..3551
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3402..3537
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3464..3483
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3599..3646
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3665..4039
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4169..4290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4206..4225)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4260..4279
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4339..4355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4339..4355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4352..4374)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4363..4379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4387..4393,4394..4411,4412..4417))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4432..4453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4570..4587)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4741..5329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4821..4840)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5500..6291,6292..6360))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6123..6142
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6361..6465)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6540..6559)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6731..7110
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7112..7310
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7328..7508
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7555..7680
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8173..8406
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8591..8635
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8933..9050
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9107..9125,9126..9148,9149..9167,9168..9190,9191..9209,
                     9210..9232,9233..9251,9252..9274,9275..9293,9294..9316,
                     9317..9335,9336..9358,9359..9377)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9410..9448
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9445..9469
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattgctagc cgatggatcc gtgaattcca taccggttac cgggtagggg aggcgctttt
       61 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
      121 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
      181 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
      241 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
      301 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
      361 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
      421 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
      481 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
      541 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
      601 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
      661 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
      721 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
      781 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
      841 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
      901 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
      961 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     1021 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     1081 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     1141 acccgcaagc ccggtgcctg aaattcgata tcaagcttat cgataatcaa cctctggatt
     1201 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     1261 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     1321 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     1381 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     1441 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     1501 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     1561 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     1621 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     1681 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     1741 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     1801 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     1861 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     1921 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     1981 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     2041 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     2101 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     2161 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     2221 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     2281 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     2341 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     2401 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     2461 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     2521 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2581 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2641 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2701 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2761 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2821 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2881 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2941 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3001 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3061 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3121 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3181 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     3241 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3301 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3361 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3421 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3481 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3541 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     3601 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     3661 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     3721 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     3781 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     3841 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     3901 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     3961 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     4021 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     4081 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4141 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     4201 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4261 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     4321 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4381 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4441 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4501 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4561 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4621 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4681 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4741 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4801 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4861 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4921 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4981 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5041 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5101 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5161 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5221 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5401 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5461 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5521 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5581 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5641 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5701 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5761 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5821 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5881 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5941 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6001 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6061 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6121 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6181 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6241 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6301 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6361 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6421 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6481 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     6541 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     6601 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     6661 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     6721 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     6781 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     6841 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     6901 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     6961 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     7021 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     7081 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     7141 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     7201 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     7261 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     7321 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7381 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7441 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7501 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7561 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     7621 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     7681 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     7741 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     7801 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     7861 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     7921 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     7981 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     8041 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8101 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8161 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8221 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     8281 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     8341 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     8401 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     8461 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     8521 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     8581 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     8641 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     8701 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     8761 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     8821 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     8881 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     8941 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9001 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9061 acagggacag cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat
     9121 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     9181 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     9241 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     9301 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     9361 cctatcagtg atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt
     9421 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     9481 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc
     9541 cg
//
