LOCUS       sequence-173537-         552 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express csgA curli protein with GS linker and Histaq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Abdelwahab MT, Kalyoncu E, Onur T, Baykara MZ, Seker UO
  TITLE     Genetically-Tunable Mechanical Properties of Bacterial Functional 
            Amyloid Nanofibers.
  JOURNAL   Langmuir. 2017 Apr 7. doi: 10.1021/acs.langmuir.7b00112.
  PUBMED    28388843
REFERENCE   2  (bases 1 to 552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Langmuir. 
            2017 Apr 7. doi: 10.1021/acs.langmuir.7b00112."
COMMENT     Sequence Label: pET22b-csgA
FEATURES             Location/Qualifiers
     source          1..552
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             472..492
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             535..552
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attgcagcaa tcgtattctc cggtagcgct ctggcaggtg ttgttcctca gtacggcggc
       61 ggcggtaacc acggtggtgg cggtaataat agcggcccaa attctgagct gaacatttac
      121 cagtacggtg gcggtaactc tgcacttgct ctgcaaactg atgcccgtaa ctctgacttg
      181 actattaccc agcatggcgg cggtaatggt gcagatgttg gtcagggctc agatgacagc
      241 tcaatcgatc tgacccaacg tggcttcggt aacagcgcta ctcttgatca gtggaacggc
      301 aaaaattctg aaatgacggt taaacagttc ggtggtggca acggtgctgc agttgaccag
      361 actgcatcta actcctccgt caacgtgact caggttggct ttggtaacaa cgcgaccgct
      421 catcagtacg gtaccggcgg tagcggtggc ggtagcggag gcggtagcgg cgaaaacctg
      481 tattttcagg gcggcggtag cggtggcggt agcggaggcg gtagcggcct cgagcaccac
      541 caccaccacc ac
//
