LOCUS       sequence-173551-        8183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the human HMG-CoA reductase isoform 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8183)
  TITLE     Genethon pCMV-SPORT6-hHMGCR1 plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-SPORT6-hHMGCR1
FEATURES             Location/Qualifiers
     source          1..8183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..70)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(71..90)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(72..90)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(101..118)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(101..117)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(110..132)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    522..656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(559..578)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     613..632
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      782..1236
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(869..888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1078..1099
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             join(1523..1591,1592..2383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1741..1760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    2485..2518
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      2596..3184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3085..3104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3338..3355
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(3429..3533)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        3643..3946
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3947..4150
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4101..4121
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4147..4171
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     4276..4292
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4276..4292
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4303..4321
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4303..4320
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    4322..4346
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6558..6569
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggcggccgct ctagagtatc cctcgagggg cccaagctta cgcgtaccca gctttcttgt
       61 acaaagtggt ccctatagtg agtcgtatta taagctaggc actggccgtc gttttacaac
      121 gtcgtgactg ggaaaactgc tagcttggga tctttgtgaa ggaaccttac ttctgtggtg
      181 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
      241 taagtgtata atgtgttaaa ctagctgcat atgcttgctg cttgagagtt ttgcttactg
      301 agtatgattt atgaaaatat tatacacagg agctagtgat tctaattgtt tgtgtatttt
      361 agattcacag tcccaaggct catttcaggc ccctcagtcc tcacagtctg ttcatgatca
      421 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
      481 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      541 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      601 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgatc
      661 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctggcgtaat
      721 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      781 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      841 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      901 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      961 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     1021 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
     1081 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1141 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1201 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     1261 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgcc
     1321 aggtcttgga ctggtgagaa cggcttgctc ggcagcttcg atgtgtgctg gagggagaat
     1381 aaaggtctga gatgtgcgat agagggaagt cgcattgaat tatgtgctgt gtagggatcg
     1441 ctggtatcaa atatgtgtgc ccacccctgg catgagacaa taaccctgat aaatgcttca
     1501 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1561 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1621 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1681 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1741 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     1801 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1861 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1921 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1981 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2041 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2101 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2161 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2221 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2281 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2341 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2401 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2461 gatccttttt gataatctca tgccataact tcgtataatg tatgctatac gaagttatgg
     2521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2701 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3001 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3241 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3301 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3361 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3421 agcttgcaat tcgcgctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3481 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3541 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3601 aataggcgta ttacgaggcc ctttcacgca ttagatgcat gtcgttacat aacttacggt
     3661 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3721 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     3781 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     3841 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     3901 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     3961 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     4021 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     4081 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     4141 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     4201 cctccataga agacaccggg accgatccag cctccggact ctagcctagg ccgcgggacg
     4261 gataacaatt tcacacagga aacagctatg accattaggc ctatttaggt gacactatag
     4321 aacaagtttg tacaaaaaag caggctggta ccggtccgga attcccggga tcgactgcgt
     4381 taactggagc caggctgagc gtcggcgccg gggttcggtg gcctctagtg agatctggag
     4441 gatccaagga ttctgtagct acaatgttgt caagactttt tcgaatgcat ggcctctttg
     4501 tggcctccca tccctgggaa gtcatagtgg ggacagtgac actgaccatc tgcatgatgt
     4561 ccatgaacat gtttactggt aacaataaga tctgtggttg gaattatgaa tgtccaaagt
     4621 ttgaagagga tgttttgagc agtgacatta taattctgac aataacacga tgcatagcca
     4681 tcctgtatat ttacttccag ttccagaatt tacgtcaact tggatcaaaa tatattttgg
     4741 gtattgctgg ccttttcaca attttctcaa gttttgtatt cagtacagtt gtcattcact
     4801 tcttagacaa agaattgaca ggcttgaatg aagctttgcc ctttttccta cttttgattg
     4861 acctttccag agcaagcaca ttagcaaagt ttgccctcag ttccaactca caggatgaag
     4921 taagggaaaa tattgctcgt ggaatggcaa ttttaggtcc tacgtttacc ctcgatgctc
     4981 ttgttgaatg tcttgtgatt ggagttggta ccatgtcagg ggtacgtcag cttgaaatta
     5041 tgtgctgctt tggctgcatg tcagttcttg ccaactactt cgtgttcatg actttcttcc
     5101 cagcttgtgt gtccttggta ttagagcttt ctcgggaaag ccgcgagggt cgtccaattt
     5161 ggcagctcag ccattttgcc cgagttttag aagaagaaga aaataagccg aatcctgtaa
     5221 ctcagagggt caagatgatt atgtctctag gcttggttct tgttcatgct cacagtcgct
     5281 ggatagctga tccttctcct caaaacagta cagcagatac ttctaaggtt tcattaggac
     5341 tggatgaaaa tgtgtccaag agaattgaac caagtgtttc cctctggcag ttttatctct
     5401 ctaaaatgat cagcatggat attgaacaag ttattaccct aagtttagct ctccttctgg
     5461 ctgtcaagta catcttcttt gaacaaacag agacagaatc tacactctca ttaaaaaacc
     5521 ctatcacatc tcctgtagtg acacaaaaga aagtcccaga caattgttgt agacgtgaac
     5581 ctatgctggt cagaaataac cagaaatgtg attcagtaga ggaagagaca gggataaacc
     5641 gagaaagaaa agttgaggtt ataaaaccct tagtggctga aacagatacc ccaaacagag
     5701 ctacatttgt ggttggtaac tcctccttac tcgatacttc atcagtactg gtgacacagg
     5761 aacctgaaat tgaacttccc agggaacctc ggcctaatga agaatgtcta cagatacttg
     5821 ggaatgcaga gaaaggtgca aaattcctta gtgatgctga gatcatccag ttagtcaatg
     5881 ctaagcatat cccagcctac aagttggaaa ctctgatgga aactcatgag cgtggtgtat
     5941 ctattcgccg acagttactt tccaagaagc tttcagaacc ttcttctctc cagtacctac
     6001 cttacaggga ttataattac tccttggtga tgggagcttg ttgtgagaat gttattggat
     6061 atatgcccat ccctgttgga gtggcaggac ccctttgctt agatgaaaaa gaatttcagg
     6121 ttccaatggc aacaacagaa ggttgtcttg tggccagcac caatagaggc tgcagagcaa
     6181 taggtcttgg tggaggtgcc agcagccgag tccttgcaga tgggatgact cgtggcccag
     6241 ttgtgcgtct tccacgtgct tgtgactctg cagaagtgaa agcctggctc gaaacatctg
     6301 aagggttcgc agtgataaag gaggcatttg acagcactag cagatttgca cgtctacaga
     6361 aacttcatac aagtatagct ggacgcaacc tttatatccg tttccagtcc aggtcagggg
     6421 atgccatggg gatgaacatg atttcaaagg gtacagagaa agcactttca aaacttcacg
     6481 agtatttccc tgaaatgcag attctagccg ttagtggtaa ctattgtact gacaagaaac
     6541 ctgctgctat aaattggata gagggaagag gaaaatctgt tgtttgtgaa gctgtcattc
     6601 cagccaaggt tgtcagagaa gtattaaaga ctaccacaga ggctatgatt gaggtcaaca
     6661 ttaacaagaa tttagtgggc tctgccatgg ctgggagcat aggaggctac aacgcccatg
     6721 cagcaaacat tgtcaccgcc atctacattg cctgtggaca ggatgcagca cagaatgttg
     6781 gtagttcaaa ctgtattact ttaatggaag caagtggtcc cacaaatgaa gatttatata
     6841 tcagctgcac catgccatct atagagatag gaacggtggg tggtgggacc aacctactac
     6901 ctcagcaagc ctgtttgcag atgctaggtg ttcaaggagc atgcaaagat aatcctgggg
     6961 aaaatgcccg gcagcttgcc cgaattgtgt gtgggaccgt aatggctggg gaattgtcac
     7021 ttatggcagc attggcagca ggacatcttg tcaaaagtca catgattcac aacaggtcga
     7081 agatcaattt acaagacctc caaggagctt gcaccaagaa gacagcctga atagcccgac
     7141 agttctgaac tggaacatgg gcattgggtt ctaaaggact aacataaaat ctgtgaatta
     7201 aaaaagctca atgcattgtc ttgtggagga tgaatagatg tgatcactga gacagccact
     7261 tggtttttgg ctctttcaga gaggtctcag gttctttcca tgcagactcc tcagatctga
     7321 acacagttta gtgctttaca tgctgtgctc tttgaagaga tttcaacaag aatattgtat
     7381 gttaaagcat cagagatggt aatctacagc tcacctctga aggcaaatat aagctgggaa
     7441 aaaagttttg atgaaattct tgaagttcat ggtgatcagt gcaattgacc ttctccctca
     7501 ctcctgccag ttgaaaatgg atttttaaat tatactgtag ctgatgaaac tcctgatttt
     7561 gtagttaatt tattaagtct gggatgtaga acttcaagaa gtaagagcta agttctaagt
     7621 tcatgtttgt aaattaatac ttcatttggt gctggtctat tttgattttg gggggtaatc
     7681 agcattattc ttcagaaggg gacctgtttt cttcaaggga agaaacactc ttattcccaa
     7741 actacagaat aatgtgttaa acatgctaaa tagttctatc aggaaaacaa atcactgtat
     7801 ttatctccgc aggctatttg ttcagagagg ccttttgttt aaatataaat gtttaaatat
     7861 aaatgtttgt ctggattggc tataacatgt ctttcagcat taggctttta agaaacacag
     7921 ggttttgtat tctttactaa agatatcaga gctcttaatg ttgcttagat gagggtgact
     7981 gtcaagtaca agcaagactg ggaccttaga aatcattgta gaaacacagt tttgaaagaa
     8041 aaataccatg tctctaagcc aactttaatt gcttaaaaga catttttatt tagttgaaaa
     8101 atctagtttt ttttgtaaac tgtatcaaat ctgtatatgt tgtaataaaa cttatgctag
     8161 tttattggaa gtgttcaaga aat
//
