LOCUS       sequence-173562-        6053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent protein localized to lipid droplets  (LDs).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Salo VT, Belevich I, Li S, Karhinen L, Vihinen H, Vigouroux C, Magre
            J, Thiele C, Holtta-Vuori M, Jokitalo E, Ikonen E
  TITLE     Seipin regulates ER-lipid droplet contacts and cargo delivery.
  JOURNAL   EMBO J. 2016 Dec 15;35(24):2699-2716. Epub 2016 Nov 22.
  PUBMED    27879284
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2016-12-15"; volume: "35(24)"; pages: "2699-2716. Epub 2016 
            Nov 22"
COMMENT     Sequence Label: pEGFP-C1-ADRP
FEATURES             Location/Qualifiers
     source          1..6053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2841..2962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2878..2897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2932..2951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2969..3424)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3106..3127)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3318..3337
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3451..3555
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3557..3914
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3557..3748
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3580..3600)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3765..3900
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3949..4743
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3949..4743
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4003..4022)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4613..4632
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4931..4950)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4975..5022
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5351..5939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5840..5859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct atggcatccg ttgcagttga tccacaaccg
     1381 agtgtggtga ctcgggtggt caacctgccc ttggtgagct ccacgtatga cctcatgtcc
     1441 tcagcctatc tcagtacaaa ggaccagtat ccctacctga agtctgtgtg tgagatggca
     1501 gagaacggtg tgaagaccat cacctccgtg gccatgacca gtgctctgcc catcatccag
     1561 aagctagagc cgcaaattgc agttgccaat acctatgcct gtaaggggct agacaggatt
     1621 gaggagagac tgcctattct gaatcagcca tcaactcaga ttgttgccaa tgccaaaggc
     1681 gctgtgactg gggcaaaaga tgctgtgacg actactgtga ctggggccaa ggattctgtg
     1741 gccagcacga tcacaggggt gatggacaag accaaagggg cagtgactgg cagtgtggag
     1801 aagaccaagt ctgtggtcag tggcagcatt aacacagtct tggggagtcg gatgatgcag
     1861 ctcgtgagca gtggcgtaga aaatgcactc accaaatcag agctgttggt agaacagtac
     1921 ctccctctca ctgaggaaga actagaaaaa gaagcaaaaa aagttgaagg atttgatctg
     1981 gttcagaagc caagttatta tgttagactg ggatccctgt ctaccaagct tcactcccgt
     2041 gcctaccagc aggctctcag cagggttaaa gaagctaagc aaaaaagcca acagaccatt
     2101 tctcagctcc attctactgt tcacctgatt gaatttgcca ggaagaatgt gtatagtgcc
     2161 aatcagaaaa ttcaggatgc tcaggataag ctctacctct catgggtaga gtggaaaagg
     2221 agcattggat atgatgatac tgatgagtcc cactgtgctg agcacattga gtcacgtact
     2281 cttgcaattg cccgcaacct gactcagcag ctccagacca cgtgccacac cctcctgtcc
     2341 aacatccaag gtgtaccaca gaacatccaa gatcaagcca agcacatggg ggtgatggca
     2401 ggcgacatct actcagtgtt ccgcaatgct gcctccttta aagaagtgtc tgacagcctc
     2461 ctcacttcta gcaaggggca gctgcagaaa atgaaggaat ctttagatga cgtgatggat
     2521 tatcttgtta acaacacgcc cctcaactgg ctggtaggtc ccttttatcc tcagctgact
     2581 gagtctcaga atgctcagga ccaaggtgca gagatggaca agagcagcca ggagacccag
     2641 cgatctgagc ataaaactca ttaagtcgag ctcaagcttc gaattctgca gtcgacggta
     2701 ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat accacatttg
     2761 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2821 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2881 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2941 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
     3001 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3061 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3121 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3181 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3241 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3301 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3361 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3421 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3481 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3541 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     3601 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3661 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3721 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3781 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3841 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3901 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     3961 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     4021 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     4081 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     4141 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     4201 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4261 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4321 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4381 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4441 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     4501 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4561 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4621 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4681 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4741 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     4801 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4861 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     4921 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     4981 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     5041 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     5101 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     5161 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     5221 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5461 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6001 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
