LOCUS       sequence-17357-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, 
            Lawrence HJ, Cleary ML, Largman C
  TITLE     Hox homeodomain proteins exhibit selective complex stabilities with 
            Pbx and DNA.
  JOURNAL   Nucleic Acids Res 1996 Mar 1;24(5):898-906.
  PUBMED    8600458
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "1996-03-1"; volume: "24(5)"; pages: "898-906"
COMMENT     Sequence Label: HOXD4 (murine) MSCV
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             45..67
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             86..103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             113..130
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             134..166
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaattc gttctagaaa taattttgtt taactttaag
       61 aaggagatat accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc
      121 gcgcggcagc catatggcta gcatgactgg tggacagcaa atgggtcgcg gatccaccat
      181 gagttcgtat atggtgaact ctaagtacgt ggaccccaag tttcctccgt gcgaggaata
      241 tttgcagggt ggctacctag gcgagcaggg agccgactac tacggcagcg gtgcacaggg
      301 cgccgacttc cagccctcgg ggctctaccc acggcccgac ttcggcgagc aacccttcgg
      361 aggcggcggg cccgggcctg gctcagcgct gcccgcgagg ggtcacggac aggagccgag
      421 cggcccggga agtcattacg gtgccccggg agagccgtgc ccagcgcccc cgcctgcgcc
      481 cctgcctggc gctcgggcct gcagccagcc caccggcccc aagcagccgc cccccgggac
      541 agcactcaag cagcccgctg tggtctaccc ttggatgaag aatgtgcacg tgaattcggc
      601 gaaccccaac tacaccggcg ggg
//
