LOCUS       sequence-17360-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, 
            Buchberg AM, Largman C
  TITLE     AbdB-like Hox proteins stabilize DNA binding by the Meis1 
            homeodomain proteins.
  JOURNAL   Mol Cell Biol 1997 Nov;17(11):6448-58.
  PUBMED    9343407
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1997-11"; volume: "17(11)"; pages: "6448-58"
COMMENT     Sequence Label: HOXA9 (murine) FLAG pET
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtngaattcc ctctgaatat tttgtttaac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggactacaa
      121 ggacgacgat gacaagctta agaggcctca ggatcccacc accggggccc tgggcaacta
      181 ctatgtggac tccttcctgc tgggcgccga cgctgctgat gagctgggtg cgggacgcta
      241 cgctccaggg accctgggtc aacccccaag gcaggcggca gctctggccg aacaccccga
      301 cttcagtcct tgcagcttcc agtccaaggc ggcggtgttt ggtgcctcgt ggaacccagt
      361 gcacgcggcg ggcgccaatg cggtgcctgc tgcagtgtat catcaccacc accaccccta
      421 cgtgcatccc caggcgcccg tggcggcggc ggcgccggac ggcaggtata tgcgctcctg
      481 gctggaaccc acgcccggtg cgctctcctt cgcgggctta ccctccagcc ggccttatgg
      541 cattaaacct gaaccgctct cggccagaag gggtgactgt cccacgcttg acactcacac
      601 tttgtccctg actgactatg cttgtggttc tcctccagtt gatagagaaa aacaacccag
      661 cgaaggcgcc ttctccgaaa acaatgccga gaatgagagc ggcggagaca agccccccat
      721 cgatcccaat aacccggtgc aactggct
//
