LOCUS       sequence-173606-        9403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Gateway-compatible expression vector backbone with an 
            N-terminal Nanoluc luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9403)
  TITLE     Taipale Lab plasmids
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.2-Nanoluc-ccdB
FEATURES             Location/Qualifiers
     source          1..9403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(44..91)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(105..604)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(153..172)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     546..569
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(637..678)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    720..844
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(885..1190)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(987..1006)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1532..2191)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2106..2125
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2245..2251,2252..2269,2270..2275))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2300..2424)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2470..2982)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     regulatory      2979..2988
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3054..3078)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3075..3278)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3104..3124)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3279..3582
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3927..3971)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4156..4389
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4882..5007
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5054..5234
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5235..5461)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5489..5509)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5489..5507)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5528..5544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5528..5544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5541..5563)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5552..5568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5576..5582,5583..5600,5601..5606))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5621..5642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5759..5776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5930..6518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6010..6029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6689..7480,7481..7549))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7312..7331
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7550..7654)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7680..8135)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7817..7838)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8029..8048
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8262..8284
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8276..8293
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8277..8293
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8303..8322
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8303..8321
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      8342..8477
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8517..8638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8528..8547)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8582..8601
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8710..8943
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(9030..9403,1..25))
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 cttcttgaga caaaggcttg gccatggttt agttcctcac cttgtcgtat tatactatgc
       61 cgatatacta tgccgatgat taattgtcaa cacgtgtcta gagtcgaaag gcccggagat
      121 gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct
      181 ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac
      241 ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg
      301 ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg
      361 agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac
      421 ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt
      481 tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa
      541 gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct
      601 acccggtagg taccatcgtc attactaacc ggtacgcgta gaatcgagac cgaggagagg
      661 gttagggata ggcttacctt cgaaccgcgg gccctctaga ctcgagttac tatagagcaa
      721 ccactttgta caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
      781 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
      841 tatggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
      901 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
      961 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
     1021 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg
     1081 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
     1141 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc
     1201 cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
     1261 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
     1321 tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg
     1381 ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg
     1441 tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt
     1501 atctggcttt tagtaagccg gatccagatc tttacgcccc gccctgccac tcatcgcagt
     1561 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1621 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1681 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1741 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1801 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1861 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1921 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cggaattccg
     1981 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     2041 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     2101 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     2161 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     2221 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
     2281 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
     2341 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     2401 ttcagctttt ttgtacaaac ttgtgcccga gcccgaggtc gagcccgagc cactcgagcg
     2461 gccgccactc gccagaatgc gttcgcacag ccgccagccg gtcactccgt tgatggttac
     2521 tcggaacagc agggagccgt cggggttgat caggcgctcg tcgataattt tgttgccgtt
     2581 ccacagggtc cctgttacag tgatcttttt gccgtcgaac acggcgatgc cttcatacgg
     2641 ccgtccgaaa tagtcgatca tgttcggcgt aaccccgtcg attaccagtg tgccatagtg
     2701 caggatcacc ttaaagtgat gatcatccac agggtacacc accttaaaaa ttttttcgat
     2761 ctggcccatt tggtcgccgc tcagaccttc atacgggatg atgacatgga tgtcgatctt
     2821 cagcccattt tcaccgctca ggacaatcct ttggatcgga gttacggaca ccccgagatt
     2881 ctgaaacaaa ctggacacac ctccctgttc aaggacttgg tccaggttgt agccggctgt
     2941 ctgtcgccag tccccaacga aatcttcgag tgtgaagacc atggtggcgg ctggatcctc
     3001 tagagtcggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga
     3061 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     3121 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     3181 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     3241 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     3301 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     3361 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     3421 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     3481 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     3541 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc ccaagcttat
     3601 cgcagaccca gtcgctagcg ttaacgtcga catcgcagac ccagtcgcta gcgttaacgt
     3661 cgacatcgat accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct
     3721 ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc
     3781 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
     3841 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
     3901 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
     3961 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
     4021 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
     4081 gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag
     4141 caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt
     4201 gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata
     4261 gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc
     4321 gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac
     4381 aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg
     4441 ggtgctactc ctaatggttc aatttttact actttatatt tatataattc acttctccaa
     4501 ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccgcttgct gtgcggtggt
     4561 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     4621 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     4681 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     4741 ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     4801 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     4861 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     4921 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     4981 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     5041 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     5101 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     5161 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     5221 aaccagagag acccgtttat gtatcgagct aggcacttaa atacaatatc tctgcaatgc
     5281 ggcaattcag tggttcgtcc aatccatgtc agacccgtct gttgccttcc taataaggca
     5341 cgatcgtacc accttacttc caccaatcgg catgcacggt gctttttctc tccttgtaag
     5401 gcatgttgct aactcatcgt taccatgttg caagactaca agagtattgc ataagactac
     5461 attaagcttg cagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     5521 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5581 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5641 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5701 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5761 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5821 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5881 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5941 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6001 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6061 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6121 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6181 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6241 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6301 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6361 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6421 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6481 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6541 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6601 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6661 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6721 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6781 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6841 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6901 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6961 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7021 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7081 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7141 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7201 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7261 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7321 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7381 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7441 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7501 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7561 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7621 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     7681 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     7741 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     7801 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     7861 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     7921 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     7981 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     8041 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     8101 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
     8161 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     8221 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     8281 aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtacgtccct
     8341 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg
     8401 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
     8461 ggagttaggg gcgggatggg cggagttagg ggcgggatag ctagagccag acatgataag
     8521 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     8581 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttcc
     8641 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     8701 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     8761 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     8821 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     8881 agagagaccc agtacaagca aaaagcagat cttgtcttcg ttgggagtga attagccctt
     8941 ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg
     9001 gtcttaaagg taccgagctc gaattgtgct tagccctccc acacataacc agagggcagc
     9061 aattcacgaa tcccaactgc cgtcggctgt ccatcactgt ccttcactat ggctttgatc
     9121 ccaggatgca gatcgagaag cacctgtcgg caccgtccgc aggggctcaa gatgcccctg
     9181 ttctcatttc cgatcgcgac gatacaagtc aggttgccag ctgccgcagc agcagcagtg
     9241 cccagcacca cgagttctgc acaaggtccc ccagtaaaat gatatacatt gacaccagtg
     9301 aagatgcggc cgtcgctaga gagagctgcg ctggcgacgc tgtagtcttc agagatgggg
     9361 atgctgttga ttgtagccgt tgctctttca atgagggtgg att
//
