LOCUS       sequence-173607-       10162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry-tagged membrane recruiter construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Ebner M, Lucic I, Leonard TA, Yudushkin I
  TITLE     PI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes.
  JOURNAL   Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 
            10.1016/j.molcel.2016.12.028.
  PUBMED    28157504
REFERENCE   2  (bases 1 to 10162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.12"; journalName: "Mol Cell"; date: 
            "2017-02-2- 2"; volume: "65"; issue: "3"; pages: "416-431.e6"
COMMENT     Sequence Label: pHR-FKBP:mCherry-KRas4Bc30
FEATURES             Location/Qualifiers
     source          1..10162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(73..93)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        78..407
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      258..393
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1541..1606
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1736..1756
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2181..2315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2218..2237)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2272..2291
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2483..3116
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3163..3288
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3785..4018
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4203..4247
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4532..4649
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4802..5209
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      5243..5252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5249..5251,5252..5572)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             5597..6304
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5742..5760)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6010..6029
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      6314..6323
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6326..6352
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6508..7096
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6561..6581)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6979..6990)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7099..7115)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7100..7116)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7206..7439
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7464..7482)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7465..7482)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7772..7791)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8005..8024)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8124..8146
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8184..8202)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8270..8374
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8375..8443,8444..9235)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8593..8612)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9406..9994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9895..9914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(10148..10162,1..3)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
       61 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
      121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      181 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      241 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      301 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      361 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
      421 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
      481 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
      541 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
      601 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
      661 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
      721 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
      781 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
      841 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
      901 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
      961 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     1021 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     1081 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     1141 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     1201 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     1261 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     1321 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
     1381 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
     1441 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
     1501 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     1561 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     1621 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     1681 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     1741 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     1801 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     1861 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     1921 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     1981 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     2041 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     2101 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2161 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2221 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2281 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     2341 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     2401 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     2461 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
     2521 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     2581 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     2641 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     2701 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     2761 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     2821 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     2881 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     2941 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     3001 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     3061 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     3121 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     3181 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     3241 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     3301 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     3361 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     3421 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     3481 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     3541 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     3601 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
     3661 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     3721 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     3781 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     3841 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     3901 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     3961 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     4021 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     4081 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     4141 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     4201 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     4261 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     4321 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     4381 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     4441 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     4501 ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     4561 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     4621 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     4681 tttggatcga taagcttgat atcgaattcc tgcagccccg ataaaataaa agattttatt
     4741 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctgc
     4801 agtaacgcca ttttgcaagg catggaaaaa taccaaacca agaatagaga agttcagatc
     4861 aagggcgggt acatgaaaat agctaacgtt gggccaaaca ggatatctgc ggtgagcagt
     4921 ttcggccccg gcccggggcc aagaacagat ggtcaccgca gtttcggccc cggcccgagg
     4981 ccaagaacag atggtcccca gatatggccc aaccctcagc agtttcttaa gacccatcag
     5041 atgtttccag gctcccccaa ggacctgaaa tgaccctgcg ccttatttga attaaccaat
     5101 cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc gagctctata aaagagctca
     5161 caacccctca ctcggcgcgc cagtcctccg acagactgag tcgcccgggg gggatctgga
     5221 gctctcgaga attctcacgc gtgccaccat gggagtgcag gtggagacta tctccccagg
     5281 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct
     5341 tgaagatgga aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct
     5401 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca
     5461 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat
     5521 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaggtgctgc
     5581 acttctgccc ggttctatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     5641 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     5701 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     5761 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     5821 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     5881 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     5941 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     6001 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     6061 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     6121 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     6181 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     6241 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     6301 caagcccggt tctgccgcca tggcatatcc atatgacgtc ccagactatg ctggcttagt
     6361 tcgagaaatt cgaaaacata aagaaaagat gagcaaagat ggtaaaaaga agaaaaagaa
     6421 gtcaaagaca aagtgtgtaa ttatgtaagc ggccgcgact ctagagtcga cctgcaggca
     6481 tgcaagcttg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     6541 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6601 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6661 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6721 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6781 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6841 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6901 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6961 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7021 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7081 tgggccgcct ccccgcatcg ataccgtcga cctcgaggga attaattcga gctcggtacc
     7141 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7201 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     7261 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7321 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7381 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7441 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     7501 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     7561 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     7621 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     7681 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     7741 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     7801 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     7861 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     7921 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     7981 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     8041 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     8101 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     8161 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     8221 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     8281 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8341 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8401 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8461 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     8521 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     8581 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8641 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8701 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8761 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8821 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8881 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8941 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     9001 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     9061 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     9121 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     9181 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     9241 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     9301 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     9361 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9421 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9481 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9541 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9601 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9661 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9721 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9781 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9841 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9901 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9961 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    10021 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    10081 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    10141 cgagcgcagc gagtcagtga gc
//
