LOCUS       sequence-17361-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, 
            Buchberg AM, Largman C
  TITLE     AbdB-like Hox proteins stabilize DNA binding by the Meis1 
            homeodomain proteins.
  JOURNAL   Mol Cell Biol 1997 Nov;17(11):6448-58.
  PUBMED    9343407
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1997-11"; volume: "17(11)"; pages: "6448-58"
COMMENT     Sequence Label: HOXA9 (murine) FLAG pRC/CMV
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             93..115
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             134..151
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             161..178
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             185..208
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctacta gagaaccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tctagaaata attttgttta actttaagaa ggagatatac
      121 catgggcagc agccatcatc atcatcatca cagcagcggc ctggtgccgc gcggcagcca
      181 tatggactac aaggacgacg atgacaagct taagaggcct caggatccca ccaccggggc
      241 cctgggcaac tactatgtgg actccttcct gctgggcgcc gacgctgctg atgagctggg
      301 tgcgggacgc tacgctccag ggaccctggg tcaaccccca aggcaggcgg cagctctggc
      361 cgaacacccc gacttcagtc cttgcagctt ccagtccaag gcggcggtgt ttggtgcctc
      421 gtggaaccca gtgcacgcgg cgggcgccaa tgcggtgcct gctgcagtgt atcatcacca
      481 ccaccacccc tacgtgcatc cccaggcgcc cgtggcggcg gcggcgccgg acggcaggta
      541 tatgcgctcc tggctggaac ccacgcccgg tgcgctctcc ttcgcgggct taccctccag
      601 ccggccttat ggcattaaac ctgaaccgct ctcggccaga aggggtgact gtcccacgct
      661 tgacactcac actttgtccc tgactgacta tgcttgtggt tctcctccag ttgatagaga
      721 aaaacaaccc agcgaaggcg ccttctccga aacaatgccg agaatgagag cggcggagac
      781 aagcccccca tcgatcccca taacccggct gccaactggc tacatgctc
//
