LOCUS       sequence-173611-       10690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry-tagged Rab7a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Ebner M, Lucic I, Leonard TA, Yudushkin I
  TITLE     PI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes.
  JOURNAL   Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 
            10.1016/j.molcel.2016.12.028.
  PUBMED    28157504
REFERENCE   2  (bases 1 to 10690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.12"; journalName: "Mol Cell"; date: 
            "2017-02-2- 2"; volume: "65"; issue: "3"; pages: "416-431.e6"
COMMENT     Sequence Label: pHR-FKBP:mCherry-Rab7a
FEATURES             Location/Qualifiers
     source          1..10690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      38..626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     527..546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     780..797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(867..887)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        872..1201
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1052..1187
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2335..2400
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2530..2550
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2975..3109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3012..3031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3066..3085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3277..3910
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3957..4082
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4579..4812
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4997..5041
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5326..5443
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5596..6003
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      6037..6046
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6043..6045,6046..6366)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             6391..7098
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6536..6554)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6804..6823
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      7108..7117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7120..7146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    7830..8418
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7883..7903)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8301..8312)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8421..8437)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8422..8438)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8528..8761
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8786..8804)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8787..8804)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9094..9113)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9327..9346)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9446..9468
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9506..9524)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9592..9696
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9697..9765,9766..10557)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9915..9934)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
       61 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      181 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      721 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
      781 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      841 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
      901 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
      961 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     1021 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     1081 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     1141 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     1201 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     1261 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     1321 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     1381 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     1441 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     1501 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     1561 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     1621 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     1681 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     1741 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     1801 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     1861 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     1921 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     1981 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     2041 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     2101 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     2161 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     2221 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     2281 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     2341 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     2401 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     2461 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     2521 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     2581 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     2641 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     2701 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     2761 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     2821 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     2881 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2941 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3001 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3061 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     3121 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     3181 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     3241 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     3301 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     3361 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     3421 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     3481 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     3541 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     3601 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     3661 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     3721 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3781 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3841 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3901 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     3961 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     4021 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     4081 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     4141 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     4201 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     4261 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     4321 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     4381 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     4441 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     4501 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     4561 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     4621 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     4681 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     4741 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     4801 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     4861 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     4921 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     4981 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     5041 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     5101 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     5161 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     5221 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     5281 gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg
     5341 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     5401 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     5461 cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa
     5521 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     5581 ggcaagctag ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata
     5641 gagaagttca gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat
     5701 ctgcggtgag cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg
     5761 gccccggccc gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc
     5821 ttaagaccca tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat
     5881 ttgaattaac caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc
     5941 tataaaagag ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc
     6001 gggggggatc tggagctctc gagaattctc acgcgtgcca ccatgggagt gcaggtggag
     6061 actatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac
     6121 tacaccggga tgcttgaaga tggaaagaaa tttgattcct cccgggacag aaacaagccc
     6181 tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag
     6241 atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta tggtgccact
     6301 gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga gcttctaaaa
     6361 ctggaaggtg ctgcacttct gcccggttct atggtgagca agggcgagga ggataacatg
     6421 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     6481 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     6541 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     6601 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     6661 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     6721 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     6781 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     6841 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     6901 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     6961 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     7021 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     7081 atggacgagc tgtacaagcc cggttctgcc gccatggcat atccatatga cgtcccagac
     7141 tatgctatga cctctaggaa gaaagtgttg ctgaaggtta tcatcctggg agattctgga
     7201 gtcgggaaga catcactcat gaaccagtat gtgaataaga aattcagcaa tcagtacaaa
     7261 gccacaatag gagctgactt tctgaccaag gaggtgatgg tggatgacag gctagtcaca
     7321 atgcagatat gggacacagc aggacaggaa cggttccagt ctctcggtgt ggccttctac
     7381 agaggtgcag actgctgcgt tctggtattt gatgtgactg cccccaacac attcaaaacc
     7441 ctagatagct ggagagatga gtttctcatc caggccagtc cccgagatcc tgaaaacttc
     7501 ccatttgttg tgttgggaaa caagattgac ctcgaaaaca gacaagtggc cacaaagcgg
     7561 gcacaggcct ggtgctacag caaaaacaac attccctact ttgagaccag tgccaaggag
     7621 gccatcaacg tggagcaggc gttccagacg attgcacgga atgcacttaa gcaggaaacg
     7681 gaggtggagc tgtacaacga atttcctgaa cctatcaaac tggacaagaa tgaccgggcc
     7741 aaggcctcgg cagaaagctg cagttgctaa gcggccgcga ctctagagtc gacctgcagg
     7801 catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     7861 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7921 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7981 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     8041 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     8101 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     8161 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     8221 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     8281 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     8341 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     8401 tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta
     8461 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8521 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     8581 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8641 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8701 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8761 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     8821 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     8881 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     8941 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     9001 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     9061 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     9121 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     9181 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     9241 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     9301 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9361 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     9421 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     9481 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     9541 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     9601 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9661 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9721 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9781 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9841 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9901 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9961 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    10021 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    10081 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    10141 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    10201 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    10261 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    10321 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10381 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10441 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10501 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10561 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10621 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10681 tcgttccact
//
