LOCUS       sequence-173628-       17172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  myo-Inositol biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17172)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB686
FEATURES             Location/Qualifiers
     source          1..17172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      425..1136
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(1626..2276)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             2382..3581
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(join(4108..4899,4900..4968))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4731..4750
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4969..5073)
                     /gene="bla"
                     /label=AmpR promoter
     oriT            5250..5358
                     /note="incP origin of transfer"
     primer_bind     5403..5421
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    5546..5567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5582..5587,5588..5605,5606..5612)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5620..5636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5625..5647
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5644..5660
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5644..5660
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             9932..11374
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             11387..12310
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             12359..13441
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             13456..14439
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             14618..15730
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(15782..16930)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga
       61 ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg cttgcggcag
      121 cgtgaagctg acggatcgat ccggggaatt aattcacccc cgaacacgag cacggcaccc
      181 gcgaccacta tgccaagaat gcccaaggta aaaattgccg gccccgccat gaagtccgtg
      241 aatgccccga cggccgaagt gaagggcagg ccgccaccca ggccgccgcc ctcactgccc
      301 ggcacctggt cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc
      361 gcgctcgggc tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc
      421 agcgctgcca tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat
      481 gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt
      541 gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt
      601 aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat
      661 gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc
      721 agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct
      781 caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact
      841 cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg
      901 aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc
      961 aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg
     1021 gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata
     1081 gacggccgcc agcccagcgg cgagggcaac cagcccggtg agcgtcggaa aggcgctgga
     1141 agccccgtag cgacgcggag aggggcgaga caagccaagg gcgcaggctc gatgcgcagc
     1201 acgacatagc cggttctcgc aaggacgaga atttccctgc ggtgcccctc aagtgtcaat
     1261 gaaagtttcc aacgcgagcc attcgcgaga gccttgagtc cacgctagat ccgtcaattc
     1321 cgggatctat ctcatctgcg caaggcagaa cgtgaagacg gccgccctgg acctcgcccg
     1381 cgagcgccag gcgcacgagg ccggcgcgcg gacccgcgcc acggcccacg agcggacgcc
     1441 gcagcaggag cgccagaagg ccgccagaga ggccgagcgc ggccgtgagg cttggacgct
     1501 agggcagggc atgaaaaagc ccgtagcggg ctgctacggg cgtctgacgc ggtggaaagg
     1561 gggaggggat gttgtctaca tggctctgct gtagtgagtg ggttgcgctc cggcagcggt
     1621 cctgatcaat cgtcaccctt tctcggtcct tcaacgttcc tgacaacgag cctccttttc
     1681 gccaatccat cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg
     1741 tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg
     1801 ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag
     1861 tagctgattg tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg
     1921 aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg
     1981 atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga agctgcgggc attcccgatc
     2041 agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc
     2101 atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc
     2161 ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc
     2221 tcgttcaaca ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgctt
     2281 gacactttat cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc
     2341 gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc aacatacccc
     2401 tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg catcggcctg attatgccgg
     2461 tgctgccggg cctcctgcgc gatctggttc actcgaacga cgtcaccgcc cactatggca
     2521 ttctgctggc gctgtatgcg ttggtgcaat ttgcctgcgc acctgtgctg ggcgcgctgt
     2581 cggatcgttt cgggcggcgg ccaatcttgc tcgtctcgct ggccggcgcc actgtcgact
     2641 acgccatcat ggcgacagcg cctttccttt gggttctcta tatcgggcgg atcgtggccg
     2701 gcatcaccgg ggcgactggg gcggtagccg gcgcttatat tgccgatatc actgatggcg
     2761 atgagcgcgc gcggcacttc ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg
     2821 gacctgtgct cggtgggctg atgggcggtt tctcccccca cgctccgttc ttcgccgcgg
     2881 cagccttgaa cggcctcaat ttcctgacgg gctgtttcct tttgccggag tcgcacaaag
     2941 gcgaacgccg gccgttacgc cgggaggctc tcaacccgct cgcttcgttc cggtgggccc
     3001 ggggcatgac cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa cttgtcggac
     3061 aggtgccggc cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg gacgcgacca
     3121 cgatcggcat ttcgcttgcc gcatttggca ttctgcattc actcgcccag gcaatgatca
     3181 ccggccctgt agccgcccgg ctcggcgaaa ggcgggcact catgctcgga atgattgccg
     3241 acggcacagg ctacatcctg cttgccttcg cgacacgggg atggatggcg ttcccgatca
     3301 tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc
     3361 aggtggatga ggaacgtcag gggcagctgc aaggctcact ggcggcgctc accagcctga
     3421 cctcgatcgt cggacccctc ctcttcacgg cgatctatgc ggcttctata acaacgtgga
     3481 acgggtgggc atggattgca ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc
     3541 gcgggctttg gagcggcgca gggcaacgag ccgatcgctg atcgtggaaa cgataggccg
     3601 gaattgacgg atctatctca tctgcgcaag gcagaacgtg aagacggccg ccctggacct
     3661 cgcccgcgag cgccaggcgc acgaggccgg cgcgcggacc cgcgccacgg cccacgagcg
     3721 gacgccgcag caggagcgcc agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg
     3781 gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg
     3841 gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt gcgctccggc
     3901 agcggtcctg atcccccgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3961 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4021 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4081 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4141 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4201 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4261 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4321 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4381 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4441 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4501 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4561 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4621 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4681 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     4741 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4801 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4861 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4921 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4981 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5041 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
     5101 gtcgatcgac ggatcttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt
     5161 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac
     5221 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg
     5281 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct
     5341 gctctgcgag gctggccgat aagctctaag aaaccattat tatcatgaca ttaacctata
     5401 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt aattccaata cgcaaaccgc
     5461 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5521 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     5581 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     5641 acacaggaaa cagctatgac catgattacg ccaagctgat cccccgatgt gtagcagtgg
     5701 cggaccatat aggcagatca gaaggcgcgg ttctcctaca tgttcgccgg tgaacgcgtt
     5761 gaggaagccg ggcagtgcct cggcaaaatc cttgcgtgta gacaagacat ctgcgtagca
     5821 gttgtcctca acaacgatgt cgaaatccaa atcggagtgc tcatcgagtc ctccgtgaac
     5881 gtaagagccg ccgatcagaa gagcgcggaa gcgaacatcg gaagcgaccg catcgcggat
     5941 gcggttcaag aaagttgcat gagcttgtgg aagtgtgctg agcataaatg attctcctag
     6001 ctgttctttg ggtaagtacg ccatcaggac gttgtgagtg gcgcgatttt tagcggctga
     6061 aatcagccct tgagcctgtc ggcaagtcgc gtcatgaggt ccatgcgctc atgcaggatc
     6121 gccacgacca acgcgggttc gcccgcacgc ggcaggcaaa aaacgtagtg gtgttcgcag
     6181 cgggccatcc gcagcgcggg aaagagttcg ctcatgtcct taaacgggcc ttcgccggcg
     6241 gcaagcctgg ctatgccctg ttccagctta gcgatatagc ggcgcacctg cgccgcgccc
     6301 cactcccggc gcgtgtagcg gatgatgccg cgtagatcgg cttcggcctc agccgtgagg
     6361 atgtaggccg tcaagcgcga tccccgctga gttcttcatc aagaatttcg ccgacgctct
     6421 tggtggacac cttgccggca agcccatcgt tgatgcggtt ccccagcatg gttttcagtt
     6481 cctgccatgc ctgatcggca tcagcgtcac cggggaacag acgttcgagg gcgtattgct
     6541 taatggtctt gccctgcaag gcggccaggg ctttcaggct ctggtgctgc tggtccgtca
     6601 tgtcgattgt caggcggctc attggataac ctccataaaa tacacgtaac cacattagca
     6661 catatgtggg cgtgaggcta cagcgcgagg cgcattaagg tcgggaaaat gcgctaggcg
     6721 catttaaatt gcgtattgct gtaatgcgcc atgccggcta gactaggccc aaatgggtat
     6781 acccaatttg accaaggggg acgcgatgag ggcggccaag cactaccgac aacttctatc
     6841 catcgacttc aacatcgagg cgctggcctt cgtgcctgga cccgacggca cacgcggccg
     6901 gcgcatccac gtcctggggc gcgaggtccg cgaccggccc ggcctggtcg agtacctttc
     6961 gccggcgttc ggctcgcggg tggcgctgga cggctactgc aaggccaatt tcgatgcagt
     7021 gctgcacctg gcgtaccccg atcatcagca atggggccac gcatgaagcg ccgaagctac
     7081 gccatgctgc gcgccgctgc cgcgctggcc gtcctggtcg ttgcctcgcc ggcatgggcc
     7141 gagctgcgcg gcgaggtcgt gcgcatcatc gacggcgaca ccatcgacgt gctggtagac
     7201 aagcagccgg tgcgcgtgcg cctggtggac attgacgcgc cggaaaagcg gcaagccttc
     7261 ggcgaacgtg cgcgccaggc gctggccggc atggtgttcc gccggcacgt cctggtcgac
     7321 gagaaggaca ccgaccgtta cggccgcacg ctgggcaccg tgtgggtcaa catggagctg
     7381 gccagccggc cgccgcagcc gcgcaacgtc aacgccgcga tggttcacca gggcatggcg
     7441 tgggcctatc gcttccacgg ccgcgcggcc gaccctgaaa tgctgcggct cgaacaggag
     7501 gcgcgaggca agcgcgtcgg cctctggtcc gatccgcacg ccgtcgagcc gtggaaatgg
     7561 cgacgcgaga gcaacaaccg gagggacgaa ggttgaaggt cgcccgcatc tacctgcgcg
     7621 ccagtacgga cgagcagaat cttgaacgcc aggagagcct tgtagcggcc acgcgggccg
     7681 ccgggtacta cgtcgccggc atctaccgcg agaaggcgtc cggcgcacgc gccgaccggc
     7741 ccgagctgct gcgcatgatc gcggacctgc aacctggtga agtcgtcgtt gcggagaaga
     7801 tcgaccgcat cagccgcttg ccgttggccg aggccgagcg cctggttgcg tcgatccggg
     7861 ccaaaggggc caagctggcc gtgcctggcg tggtggacct gtcggagctg gccgccgagg
     7921 cgaacggagt ggcgaaaatc gttctggaat ccgtccagga catgcttttg aagctcgcct
     7981 tgcagatggc ccgcgacgac tacgaggatc ggcgcgagcg tcaacgtcag ggtgtccagt
     8041 tggcgaaggc cgccggccgc tacaccggcc gcaaacgtga cgccggcatg cacgaccgca
     8101 tcatcacgct tcgctccggc ggatcgagca ttgccaagac ggccaagctg gtcggatgca
     8161 gcccgagcca ggtcaaacga gtgtgggcgg cctggaacgc gcagcagcaa aaataaagcc
     8221 gggcagtgcc cggcttttct caccttttcg cgtcccgcag ggccgctgcg agcgccctac
     8281 ctagatcctc gctttccccc tcggtgtagt ccggccaggg cacgaagggc gcggatgcga
     8341 acctgttgag caggtacgcc ttcgggcagc ggtagaccac cggcgagttc gccttttcat
     8401 cccaccgggc caggatcacg tccgcatcgc agtgcatgtc cttcacctgg tcgcggaaga
     8461 agccgaaggc caccatgccg ctatgttcgc cgaggaacgc cagttgcttc gcgctggcga
     8521 tcgcgccgac gccgccggcc aaaaccgacg ccatcaccca gccgacgaac cagaagctgg
     8581 catgcttgcg gttgaccacc gcacgcgcag ccgcgaccag gacaacggcc aagctgccga
     8641 ccagggccat gacgaccgtg atccggccgt tgtggaaagc gatgggcttg ccagcgtccg
     8701 cttgcacggc gtcgtaaatg ctggacccga tgggcgcgca catcagcacg acaggcagca
     8761 gcaccaggaa catcgtccgc gtccattgcg cgagtgcctt gcggcgttcg ccggcggcaa
     8821 gcgcctccat catcggcgtg aagcccaaca gggccaccgc agccgccaag ccggcaacga
     8881 tgccgcaggc gattacatac atacatcctc cctaatgcgc cttgcgcacg gttgtagtca
     8941 gagtccgcgg tgggggtacc aggcgagcag tcaccaatat agcaggatca atattctttt
     9001 ccttcgcata ggcctcggcc ttgtcgaggt aggttttcag gctgttgagg ccgcgctgga
     9061 acatcggaac ggtgaggcga tacatcgaaa tggacatttg tcctgtctcc aaaatcttca
     9121 aatgattggg tcgcggcgag aggtcgaagg cgtctgcggc cgagagcctt tcttcgaccc
     9181 tcccatagat ggatgctcgt ttcgtctttc acaacaccga caatgcgggt gtcggtcctg
     9241 aagcctcgca tggcattgcg ctctcctcca tatctcgccg ccgctaaaga tacttgacgt
     9301 cgttcccaga aaatggaatg aatattccgc agaacagatt ttacggtttg tttgttccgt
     9361 tttgcggagc gccgcgggag aggtggcgcg gggaattgcg gcttcagagc aaatgcctgg
     9421 agctccggcg tgaggagggt gcagccgggc accgctttgt ggaggaagtg aaaatgaaaa
     9481 agtttatcct gggcaccgcg atggcgctcg tcctgtcgac ggccgctcac gcggaaactg
     9541 tcggcgtctc gatggcgaaa ttcgacgaca atttcctgac ggttctgcgc aacggcatga
     9601 ccgattatgc aaagacactg agcggcgtga cgctgcaggt cgaagacgca cagaacgacg
     9661 tttccaagca gcagagccag atccagaatt tcatcgcctc caaggtcgat gcaatcatcg
     9721 tcaaccctgt cgataccgat gcgaccacgg cgatgtcgaa gctcgcggcc gatgccggca
     9781 ttccgctggt ttacgtcaac cgccagccgg tcaacgtcga cacgctgccg gacaatcagg
     9841 ccttcgttgc ttccaacgag caggaatccg gcacgctgga aaccaaggaa atctggatcc
     9901 gtttaaacga attcaggctt ggaggatacg tatgactaaa aaaatttcat tcattattaa
     9961 cggccaggtt gaaatctttc ccgaaggtga tgatttagtg caatccatta attttggtga
    10021 taatagtgtt tacctgccaa tattgaatga ctctcatgta aaaaacatta ttgattgtaa
    10081 tggaaataac gaattacggt tgcataacat tgtcaatttt ctctatacgg tagggcaaag
    10141 atggaaaaat gaagaatact caagacgcag gacatacatt cgtgacttaa aaaaatatat
    10201 gggatattca gaagaaatgg ctaagctaga ggccaattgg atatctatga ttttatgttc
    10261 taaaggcggc ctttatgatg ttgtagaaaa tgaacttggt tctcgccata tcatggatga
    10321 atggctacct caggatgaaa gttatgttcg ggcttttccg aaaggtaaat ctgtacatct
    10381 gttggcaggt aatgttccat tatctgggat catgtctata ttacgcgcaa ttttaactaa
    10441 gaatcagtgt attataaaaa catcgtcaac cgatcctttt accgctaatg cattagcgtt
    10501 aagttttatt gatgtagacc ctaatcatcc gataacgcgc tctttatctg ttatatattg
    10561 gccccaccaa ggtgatacat cactcgcaaa agaaattatg caacatgcgg atgttattgt
    10621 cgcttgggga gggccagatg cgattaattg ggcggtagag catgcgccat cttatgctga
    10681 tgtgattaaa tttggttcta aaaagagtct ttgcattatc gataatcctg ttgatttgac
    10741 gtccgcagcg acaggtgcgg ctcatgatgt ttgtttttac gatcagcgag cttgtttttc
    10801 tgcccaaaac atatattaca tgggaaatca ttatgaggaa tttaagttag cgttgataga
    10861 aaaacttaat ctatatgcgc atatattacc gaatgccaaa aaagattttg atgaaaaggc
    10921 ggcctattct ttagttcaaa aagaaagctt gtttgctgga ttaaaagtag aggtggatat
    10981 tcatcaacgt tggatgatta ttgagtcaaa tgcaggtgtg gaatttaatc aaccacttgg
    11041 cagatgtgtg taccttcatc acgtcgataa tattgagcaa atattgcctt atgttcaaaa
    11101 aaataagacg caaaccatat ctatttttcc ttgggagtca tcatttaaat atcgagatgc
    11161 gttagcatta aaaggtgcgg aaaggattgt agaagcagga atgaataaca tatttcgagt
    11221 tggtggatct catgacggaa tgagaccgtt gcaacgatta gtgacatata tttctcatga
    11281 aaggccatct aactatacgg ctaaggatgt tgcggttgaa atagaacaga ctcgattcct
    11341 ggaagaagat aagttccttg tatttgtccc ataataggta aaaagtatgg aaaatgaatc
    11401 aaaatataaa accatcgacc acgttatttg tgttgaagga aataaaaaaa ttcatgtttg
    11461 ggaaacgctg ccagaagaaa acagcccaaa gagaaagaat gccattatta ttgcgtctgg
    11521 ttttgcccgc aggatggatc attttgctgg tctggcggaa tatttatcgc ggaatggatt
    11581 tcatgtgatc cgctatgatt cgcttcacca cgttggattg agttcaggga caattgatga
    11641 atttacaatg tctataggaa agcagagctt gttagcagtg gttgattggt taactacacg
    11701 aaaaataaat aacttcggta tgttggcttc aagcttatct gcgcggatag cttatgcaag
    11761 cctatctgaa atcaatgctt cgtttttaat caccgcagtc ggtgttgtta acttaagata
    11821 ttctcttgaa agagctttag ggtttgatta tctcagtcta cccattaatg aattgccgaa
    11881 taatctagat tttgaaggcc ataaattggg tgctgaagtc tttgcgagag attgtcttga
    11941 ttttggttgg gaagatttag cttctacaat taataacatg atgtatcttg atataccgtt
    12001 tattgctttt actgcaaata acgataattg ggtcaagcaa gatgaagtta tcacattgtt
    12061 atcaaatatt cgtagtaatc gatgcaagat atattctttg ttaggaagtt cgcatgactt
    12121 gagtgaaaat ttagtggtcc tgcgcaattt ttatcaatcg gttacgaaag ccgctatcgc
    12181 gatggataat gatcatctgg atattgatgt tgatattact gaaccgtcat ttgaacattt
    12241 aactattgcg acagtcaatg aacgccgaat gagaattgag attgaaaatc aagcaatttc
    12301 tctgtcttaa aatctattga gatattctat cactcaaata gcaatataag gactctctat
    12361 gaaatttgga aactttttgc ttacatacca acctccccaa ttttctcaaa cagaggtaat
    12421 gaaacgtttg gttaaattag gtcgcatctc tgaggagtgt ggttttgata ccgtatggtt
    12481 actggagcat catttcacgg agtttggttt gcttggtaac ccttatgtcg ctgctgcata
    12541 tttacttggc gcgactaaaa aattgaatgt aggaactgcc gctattgttc ttcccacagc
    12601 ccatccagta cgccaacttg aagatgtgaa tttattggat caaatgtcaa aaggacgatt
    12661 tcggtttggt atttgccgag ggctttacaa caaggacttt cgcgtattcg gcacagatat
    12721 gaataacagt cgcgccttag cggaatgctg gtacgggctg ataaagaatg gcatgacaga
    12781 gggatatatg gaagctgata atgaacatat caagttccat aaggtaaaag taaaccccgc
    12841 ggcgtatagc agaggtggcg caccggttta tgtggtggct gaatcagctt cgacgactga
    12901 gtgggctgct caatttggcc taccgatgat attaagttgg attataaata ctaacgaaaa
    12961 gaaagcacaa cttgagcttt ataatgaagt ggctcaagaa tatgggcacg atattcataa
    13021 tatcgaccat tgcttatcat atataacatc tgtagatcat gactcaatta aagcgaaaga
    13081 gatttgccgg aaatttctgg ggcattggta tgattcttat gtgaatgcta cgactatttt
    13141 tgatgattca gaccaaacaa gaggttatga tttcaataaa gggcagtggc gtgactttgt
    13201 attaaaagga cataaagata ctaatcgccg tattgattac agttacgaaa tcaatcccgt
    13261 gggaacgccg caggaatgta ttgacataat tcaaaaagac attgatgcta caggaatatc
    13321 aaatatttgt tgtggatttg aagctaatgg aacagtagac gaaattattg cttccatgaa
    13381 gctcttccag tctgatgtca tgccatttct taaagaaaaa caacgttcgc tattatatta
    13441 gctaaggaga aagaaatgaa atttggattg ttcttcctta acttcatcaa ttcaacaact
    13501 gttcaagaac aaagtatagt tcgcatgcag gaaataacgg agtatgttga taagttgaat
    13561 tttgaacaga ttttagtgta tgaaaatcat ttttcagata atggtgttgt cggcgctcct
    13621 ctgactgttt ctggttttct gctcggttta acagagaaaa ttaaaattgg ttcattaaat
    13681 cacatcatta caactcatca tcctgtccgc atagcggagg aagcttgctt attggatcag
    13741 ttaagtgaag ggagatttat tttagggttt agtgattgcg aaaaaaaaga tgaaatgcat
    13801 ttttttaatc gcccggttga atatcaacag caactatttg aagagtgtta tgaaatcatt
    13861 aacgatgctt taacaacagg ctattgtaat ccagataacg atttttatag cttccctaaa
    13921 atatctgtaa atccccatgc ttatacgcca ggcggacctc ggaaatatgt aacagcaacc
    13981 agtcatcata ttgttgagtg ggcggccaaa aaaggtattc ctctcatctt taagtgggat
    14041 gattctaatg atgttagata tgaatatgct gaaagatata aagccgttgc ggataaatat
    14101 gacgttgacc tatcagagat agaccatcag ttaatgatat tagttaacta taacgaagat
    14161 agtaataaag ctaaacaaga gacgcgtgca tttattagtg attatgttct tgaaatgcac
    14221 cctaatgaaa atttcgaaaa taaacttgaa gaaataattg cagaaaacgc tgtcggaaat
    14281 tatacggagt gtataactgc ggctaagttg gcaattgaaa agtgtggtgc gaaaagtgta
    14341 ttgctgtcct ttgaaccaat gaatgatttg atgagccaaa aaaatgtaat caatattgtt
    14401 gatgataata ttaagaagta ccacatggaa tatacctaat agatttcgag ttgcagcgag
    14461 gcggcaagtg aacgaatccc caggagcata gataactatg tgactggggt gagtgaaagc
    14521 agccaacaaa gcagcagctt gaaagatgaa gggtataaaa gagtatgaca gcagtgctgc
    14581 catactttct aatattatct tgaggagtaa aacaggtatg acttcatatg ttgataaaca
    14641 agaaattaca gcaagctcag aaattgatga tttgattttt tcgagcgatc cattagtgtg
    14701 gtcttacgac gagcaggaaa aaatcagaaa gaaacttgtg cttgatgcat ttcgtaatca
    14761 ttataaacat tgtcgagaat atcgtcacta ctgtcaggca cacaaagtag atgacaatat
    14821 tacggaaatt gatgacatac ctgtattccc aacatcggtt tttaagttta ctcgcttatt
    14881 aacttctcag gaaaacgaga ttgaaagttg gtttaccagt agcggcacga atggtttaaa
    14941 aagtcaggtg gcgcgtgaca gattaagtat tgagagactc ttaggctctg tgagttatgg
    15001 catgaaatat gttggtagtt ggtttgatca tcaaatagaa ttagtcaatt tgggaccaga
    15061 tagatttaat gctcataata tttggtttaa atatgttatg agtttggtgg aattgttata
    15121 tcctacgaca tttaccgtaa cagaagaacg aatagatttt gttaaaacat tgaatagtct
    15181 tgaacgaata aaaaatcaag ggaaagatct ttgtcttatt ggttcgccat actttattta
    15241 tttactctgc cattatatga aagataaaaa aatctcattt tctggagata aaagccttta
    15301 tatcataacc ggaggcggct ggaaaagtta cgaaaaagaa tctctgaaac gtgatgattt
    15361 caatcatctt ttatttgata ctttcaatct cagtgatatt agtcagatcc gagatatatt
    15421 taatcaagtt gaactcaaca cttgtttctt tgaggatgaa atgcagcgta aacatgttcc
    15481 gccgtgggta tatgcgcgag cgcttgatcc tgaaacgttg aaacctgtac ctgatggaac
    15541 gccggggttg atgagttata tggatgcgtc agcaaccagt tatccagcat ttattgttac
    15601 cgatgatgtc gggataatta gcagagaata tggtaagtat cccggcgtgc tcgttgaaat
    15661 tttacgtcgc gtcaatacga ggacgcagaa agggtgtgct ttaagcttaa ccgaagcgtt
    15721 tgatagttga tgacctgcag gtggctgctg aacccccagc cggaactgac cccacaaggc
    15781 cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc
    15841 ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat
    15901 ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc
    15961 tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga
    16021 atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc
    16081 aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt
    16141 ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc
    16201 attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct
    16261 cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct
    16321 gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca
    16381 cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt
    16441 tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg
    16501 gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt
    16561 tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt
    16621 ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg
    16681 ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag
    16741 ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc
    16801 cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg
    16861 catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg
    16921 tccgattcat tcaccctcct tgcgggattg ccccggaatt ccccggatcg atccgtcgat
    16981 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
    17041 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
    17101 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
    17161 gcgcttgcgt tt
//
