LOCUS       sequence-17363-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, 
            Buchberg AM, Largman C
  TITLE     AbdB-like Hox proteins stabilize DNA binding by the Meis1 
            homeodomain proteins.
  JOURNAL   Mol Cell Biol 1997 Nov;17(11):6448-58.
  PUBMED    9343407
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1997-11"; volume: "17(11)"; pages: "6448-58"
COMMENT     Sequence Label: HOXA11 (murine) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             92..124
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatggctagc atgactggtg gacagcaaat
      121 gggtcgcgga tccgaattca agccgcgccc aatgacatac tcctactcct ccaacctgcc
      181 ccaggtccaa cccgtgcgcg aagtgacctt cagagagtac gccattgagc ccgccactaa
      241 atggcacccc cgcggcaatc tggcccactg ctactccgcg gaggagctcg tgcacagaga
      301 ctgtctgcag gcgcccagcg cggccggcgt gcctggcgac gtgctggcca agagctcggc
      361 caacgtctac caccacccca cccccgccgt ctcgtccaat ttctatagca cggtgggcag
      421 gaacggcgtt ctgccacagg ctttcgacca gtttttcgag acggcttacg gcaccccgga
      481 aaacctcgct tcctccgact accccgggga caagaacgcc gagaaggggc cccaagcagc
      541 agctgcgacc tccgctgcgg cggtggcggc ggcggccacg ggcgcgccgg caacttcaag
      601 ttcggacggc ggcggtggcg gcggctgtca ggaggcggcg gcggaggaga aggagcgacg
      661 gcggcgaccc gagagcagca gcagccccga gtcgtcttcc ggccacactg aggacaaggc
      721 cggtggctcc ggtggccaac gcacccgcaa aaagcgctgc ccttatacca aataccagat
      781 ccgagagctg gagcgagagt tcttcttcag cgtctacatc aacaaagaga agcgtctgca
      841 actgtcccgc atgctcaacc tcaccgaccg tcaagtcaaa atctggtttt cagaacagaa
      901 gaatgaagga aaaaagatta ccagagaccg gttacagtac tactcagcta atccacttct
      961 ctaaggctcc agcctactg
//
