LOCUS       sequence-173630-       17411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hesperetin biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17411)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB712
FEATURES             Location/Qualifiers
     source          1..17411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1717..1733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1717..1733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1730..1752)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1741..1757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1765..1771,1772..1789,1790..1795))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1810..1831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1956..1974)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            2019..2127
                     /note="incP origin of transfer"
     promoter        2304..2408
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2409..2477,2478..3269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2627..2646)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3796..4995)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             5101..5751
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      6241..6952
                     /label=oriV
                     /note="incP origin of replication"
     CDS             7619..8767
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8819..9931)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(10110..11093)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(11108..12190)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(12239..13162)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(13175..14617)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 tccatgttga cccacacggt gcccagcgtg cggccgtaac ggtcggtgtc cttctcgtcg
       61 accaggacgt gccggcggaa caccatgccg gccagcgcct ggcgcgcacg ttcgccgaag
      121 gcttgccgct tttccggcgc gtcaatgtcc accaggcgca cgcgcaccgg ctgcttgtct
      181 accagcacgt cgatggtgtc gccgtcgatg atgcgcacga cctcgccgcg cagctcggcc
      241 catgccggcg aggcaacgac caggacggcc agcgcggcag cggcgcgcag catggcgtag
      301 cttcggcgct tcatgcgtgg ccccattgct gatgatcggg gtacgccagg tgcagcactg
      361 catcgaaatt ggccttgcag tagccgtcca gcgccacccg cgagccgaac gccggcgaaa
      421 ggtactcgac caggccgggc cggtcgcgga cctcgcgccc caggacgtgg atgcgccggc
      481 cgcgtgtgcc gtcgggtcca ggcacgaagg ccagcgcctc gatgttgaag tcgatggata
      541 gaagttgtcg gtagtgcttg gccgccctca tcgcgtcccc cttggtcaaa ttgggtatac
      601 ccatttgggc ctagtctagc cggcatggcg cattacagca atacgcaatt taaatgcgcc
      661 tagcgcattt tcccgacctt aatgcgcctc gcgctgtagc ctcacgccca catatgtgct
      721 aatgtggtta cgtgtatttt atggaggtta tccaatgagc cgcctgacaa tcgacatgac
      781 ggaccagcag caccagagcc tgaaagccct ggccgccttg cagggcaaga ccattaagca
      841 atacgccctc gaacgtctgt tccccggtga cgctgatgcc gatcaggcat ggcaggaact
      901 gaaaaccatg ctggggaacc gcatcaacga tgggcttgcc ggcaaggtgt ccaccaagag
      961 cgtcggcgaa attcttgatg aagaactcag cggggatcgc gcttgacggc ctacatcctc
     1021 acggctgagg ccgaagccga tctacgcggc atcatccgct acacgcgccg ggagtggggc
     1081 gcggcgcagg tgcgccgcta tatcgctaag ctggaacagg gcatagccag gcttgccgcc
     1141 ggcgaaggcc cgtttaagga catgagcgaa ctctttcccg cgctgcggat ggcccgctgc
     1201 gaacaccact acgttttttg cctgccgcgt gcgggcgaac ccgcgttggt cgtggcgatc
     1261 ctgcatgagc gcatggacct catgacgcga cttgccgaca ggctcaaggg ctgatttcag
     1321 ccgctaaaaa tcgcgccact cacaacgtcc tgatggcgta cttacccaaa gaacagctag
     1381 gagaatcatt tatgctcagc acacttccac aagctcatgc aactttcttg aaccgcatcc
     1441 gcgatgcggt cgcttccgat gttcgcttcc gcgctcttct gatcggcggc tcttacgttc
     1501 acggaggact cgatgagcac tccgatttgg atttcgacat cgttgttgag gacaactgct
     1561 acgcagatgt cttgtctaca cgcaaggatt ttgccgaggc actgcccggc ttcctcaacg
     1621 cgttcaccgg cgaacatgta ggagaaccgc gccttctgat ctgcctatat ggtccgccac
     1681 tgctacacat cgggggatca gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1741 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1801 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1861 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1921 tttgcgtatt ggaattaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     1981 ggttaatgtc atgataataa tggtttctta gagcttatcg gccagcctcg cagagcagga
     2041 ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg
     2101 ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct
     2161 acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa
     2221 atcgctataa tgaccccgaa gcagggttat gcagcggaaa agatccgtcg atcgacccag
     2281 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2341 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2401 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     2461 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     2521 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2581 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     2641 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     2701 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     2761 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     2821 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     2881 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     2941 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     3001 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     3061 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     3121 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     3181 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     3241 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     3301 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     3361 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3421 aaaagatcaa aggatcttct tgagatcctt tttttctgcg ggggatcagg accgctgccg
     3481 gagcgcaacc cactcactac agcagagcca tgtagacaac atcccctccc cctttccacc
     3541 gcgtcagacg cccgtagcag cccgctacgg gctttttcat gccctgccct agcgtccaag
     3601 cctcacggcc gcgctcggcc tctctggcgg ccttctggcg ctcctgctgc ggcgtccgct
     3661 cgtgggccgt ggcgcgggtc cgcgcgccgg cctcgtgcgc ctggcgctcg cgggcgaggt
     3721 ccagggcggc cgtcttcacg ttctgccttg cgcagatgag atagatccgt caattccggc
     3781 ctatcgtttc cacgatcagc gatcggctcg ttgccctgcg ccgctccaaa gcccgcgacg
     3841 cagcgccggc aggcagagca agtagagggc agcgcctgca atccatgccc acccgttcca
     3901 cgttgttata gaagccgcat agatcgccgt gaagaggagg ggtccgacga tcgaggtcag
     3961 gctggtgagc gccgccagtg agccttgcag ctgcccctga cgttcctcat ccacctgcct
     4021 ggacaacatt gcttgcagcg ccggcattcc gatgccaccc gaagcaagca ggaccatgat
     4081 cgggaacgcc atccatcccc gtgtcgcgaa ggcaagcagg atgtagcctg tgccgtcggc
     4141 aatcattccg agcatgagtg cccgcctttc gccgagccgg gcggctacag ggccggtgat
     4201 cattgcctgg gcgagtgaat gcagaatgcc aaatgcggca agcgaaatgc cgatcgtggt
     4261 cgcgtcccag tgaaagcgat cctcgccgaa aatgacccaa agcgcggccg gcacctgtcc
     4321 gacaagttgc atgatgaaga agaccgccat cagggcggcg acgacggtca tgccccgggc
     4381 ccaccggaac gaagcgagcg ggttgagagc ctcccggcgt aacggccggc gttcgccttt
     4441 gtgcgactcc ggcaaaagga aacagcccgt caggaaattg aggccgttca aggctgccgc
     4501 ggcgaagaac ggagcgtggg gggagaaacc gcccatcagc ccaccgagca caggtcccgc
     4561 gaccatcccg aacccgaaac aggcgctcat gaagccgaag tgccgcgcgc gctcatcgcc
     4621 atcagtgata tcggcaatat aagcgccggc taccgcccca gtcgccccgg tgatgccggc
     4681 cacgatccgc ccgatataga gaacccaaag gaaaggcgct gtcgccatga tggcgtagtc
     4741 gacagtggcg ccggccagcg agacgagcaa gattggccgc cgcccgaaac gatccgacag
     4801 cgcgcccagc acaggtgcgc aggcaaattg caccaacgca tacagcgcca gcagaatgcc
     4861 atagtgggcg gtgacgtcgt tcgagtgaac cagatcgcgc aggaggcccg gcagcaccgg
     4921 cataatcagg ccgatgccga cagcgtcgag cgcgacagtg ctcagaatta cgatcagggg
     4981 tatgttgggt ttcacgtctg gcctccggac cagcctccgc tggtccgatt gaacgcgcgg
     5041 attctttatc actgataagt tggtggacat attatgttta tcagtgataa agtgtcaagc
     5101 atgacaaagt tgcagccgaa tacagtgatc cgtgccgccc tggacctgtt gaacgaggtc
     5161 ggcgtagacg gtctgacgac acgcaaactg gcggaacggt tgggggttca gcagccggcg
     5221 ctttactggc acttcaggaa caagcgggcg ctgctcgacg cactggccga agccatgctg
     5281 gcggagaatc atacgcattc ggtgccgaga gccgacgacg actggcgctc atttctgatc
     5341 gggaatgccc gcagcttcag gcaggcgctg ctcgcctacc gcgatggcgc gcgcatccat
     5401 gccggcacgc gaccgggcgc accgcagatg gaaacggccg acgcgcagct tcgcttcctc
     5461 tgcgaggcgg gtttttcggc cggggacgcc gtcaatgcgc tgatgacaat cagctacttc
     5521 actgttgggg ccgtgcttga ggagcaggcc ggcgacagcg atgccggcga gcgcggcggc
     5581 accgttgaac aggctccgct ctcgccgctg ttgcgggccg cgatagacgc cttcgacgaa
     5641 gccggtccgg acgcagcgtt cgagcaggga ctcgcggtga ttgtcgatgg attggcgaaa
     5701 aggaggctcg ttgtcaggaa cgttgaagga ccgagaaagg gtgacgattg atcaggaccg
     5761 ctgccggagc gcaacccact cactacagca gagccatgta gacaacatcc cctccccctt
     5821 tccaccgcgt cagacgcccg tagcagcccg ctacgggctt tttcatgccc tgccctagcg
     5881 tccaagcctc acggccgcgc tcggcctctc tggcggcctt ctggcgctcc tgctgcggcg
     5941 tccgctcgtg ggccgtggcg cgggtccgcg cgccggcctc gtgcgcctgg cgctcgcggg
     6001 cgaggtccag ggcggccgtc ttcacgttct gccttgcgca gatgagatag atcccggaat
     6061 tgacggatct agcgtggact caaggctctc gcgaatggct cgcgttggaa actttcattg
     6121 acacttgagg ggcaccgcag ggaaattctc gtccttgcga gaaccggcta tgtcgtgctg
     6181 cgcatcgagc ctgcgccctt ggcttgtctc gcccctctcc gcgtcgctac ggggcttcca
     6241 gcgcctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
     6301 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
     6361 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
     6421 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
     6481 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
     6541 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
     6601 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
     6661 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
     6721 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
     6781 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
     6841 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
     6901 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agcgctggca
     6961 gtccttgcca ttgccgggat cggggcagta acgggatggg cgatcagccc gagcgcgacg
     7021 cccggaagca ttgacgtgcc gcaggtgctg gcatcgacat tcagcgacca ggtgccgggc
     7081 agtgagggcg gcggcctggg tggcggcctg cccttcactt cggccgtcgg ggcattcacg
     7141 gacttcatgg cggggccggc aatttttacc ttgggcattc ttggcatagt ggtcgcgggt
     7201 gccgtgctcg tgttcggggg tgaattaatt ccccggatcg atccgtcagc ttcacgctgc
     7261 cgcaagcact cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg
     7321 cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac
     7381 gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg
     7441 gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt
     7501 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     7561 ggatcaagat cgacggatcg atccggggaa ttccggggca atcccgcaag gagggtgaat
     7621 gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg ggttttccgc
     7681 cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca
     7741 gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca gcgtgcaact
     7801 ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc gtctcgaaca
     7861 ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta tgacgaccaa
     7921 gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca agcaggccgc
     7981 gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt tcgatattgc
     8041 gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg ccctgttcac
     8101 cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa
     8161 caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg acgaactggt
     8221 gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga tcaccttcac
     8281 gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt acacgaaggc
     8341 cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg
     8401 gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac
     8461 gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta
     8521 cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac ggatgttcga
     8581 ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg
     8641 cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga
     8701 gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa
     8761 acgctagggc cttgtggggt cagttccggc tgggggttca gcagccacct gcaggtcatc
     8821 aactatcaaa cgcttcggtt aagcttaaag cacacccttt ctgcgtcctc gtattgacgc
     8881 gacgtaaaat ttcaacgagc acgccgggat acttaccata ttctctgcta attatcccga
     8941 catcatcggt aacaataaat gctggataac tggttgctga cgcatccata taactcatca
     9001 accccggcgt tccatcaggt acaggtttca acgtttcagg atcaagcgct cgcgcatata
     9061 cccacggcgg aacatgttta cgctgcattt catcctcaaa gaaacaagtg ttgagttcaa
     9121 cttgattaaa tatatctcgg atctgactaa tatcactgag attgaaagta tcaaataaaa
     9181 gatgattgaa atcatcacgt ttcagagatt ctttttcgta acttttccag ccgcctccgg
     9241 ttatgatata aaggctttta tctccagaaa atgagatttt tttatctttc atataatggc
     9301 agagtaaata aataaagtat ggcgaaccaa taagacaaag atctttccct tgatttttta
     9361 ttcgttcaag actattcaat gttttaacaa aatctattcg ttcttctgtt acggtaaatg
     9421 tcgtaggata taacaattcc accaaactca taacatattt aaaccaaata ttatgagcat
     9481 taaatctatc tggtcccaaa ttgactaatt ctatttgatg atcaaaccaa ctaccaacat
     9541 atttcatgcc ataactcaca gagcctaaga gtctctcaat acttaatctg tcacgcgcca
     9601 cctgactttt taaaccattc gtgccgctac tggtaaacca actttcaatc tcgttttcct
     9661 gagaagttaa taagcgagta aacttaaaaa ccgatgttgg gaatacaggt atgtcatcaa
     9721 tttccgtaat attgtcatct actttgtgtg cctgacagta gtgacgatat tctcgacaat
     9781 gtttataatg attacgaaat gcatcaagca caagtttctt tctgattttt tcctgctcgt
     9841 cgtaagacca cactaatgga tcgctcgaaa aaatcaaatc atcaatttct gagcttgctg
     9901 taatttcttg tttatcaaca tatgaagtca tacctgtttt actcctcaag ataatattag
     9961 aaagtatggc agcactgctg tcatactctt ttataccctt catctttcaa gctgctgctt
    10021 tgttggctgc tttcactcac cccagtcaca tagttatcta tgctcctggg gattcgttca
    10081 cttgccgcct cgctgcaact cgaaatctat taggtatatt ccatgtggta cttcttaata
    10141 ttatcatcaa caatattgat tacatttttt tggctcatca aatcattcat tggttcaaag
    10201 gacagcaata cacttttcgc accacacttt tcaattgcca acttagccgc agttatacac
    10261 tccgtataat ttccgacagc gttttctgca attatttctt caagtttatt ttcgaaattt
    10321 tcattagggt gcatttcaag aacataatca ctaataaatg cacgcgtctc ttgtttagct
    10381 ttattactat cttcgttata gttaactaat atcattaact gatggtctat ctctgatagg
    10441 tcaacgtcat atttatccgc aacggcttta tatctttcag catattcata tctaacatca
    10501 ttagaatcat cccacttaaa gatgagagga ataccttttt tggccgccca ctcaacaata
    10561 tgatgactgg ttgctgttac atatttccga ggtccgcctg gcgtataagc atggggattt
    10621 acagatattt tagggaagct ataaaaatcg ttatctggat tacaatagcc tgttgttaaa
    10681 gcatcgttaa tgatttcata acactcttca aatagttgct gttgatattc aaccgggcga
    10741 ttaaaaaaat gcatttcatc ttttttttcg caatcactaa accctaaaat aaatctccct
    10801 tcacttaact gatccaataa gcaagcttcc tccgctatgc ggacaggatg atgagttgta
    10861 atgatgtgat ttaatgaacc aattttaatt ttctctgtta aaccgagcag aaaaccagaa
    10921 acagtcagag gagcgccgac aacaccatta tctgaaaaat gattttcata cactaaaatc
    10981 tgttcaaaat tcaacttatc aacatactcc gttatttcct gcatgcgaac tatactttgt
    11041 tcttgaacag ttgttgaatt gatgaagtta aggaagaaca atccaaattt catttctttc
    11101 tccttagcta atataatagc gaacgttgtt tttctttaag aaatggcatg acatcagact
    11161 ggaagagctt catggaagca ataatttcgt ctactgttcc attagcttca aatccacaac
    11221 aaatatttga tattcctgta gcatcaatgt ctttttgaat tatgtcaata cattcctgcg
    11281 gcgttcccac gggattgatt tcgtaactgt aatcaatacg gcgattagta tctttatgtc
    11341 cttttaatac aaagtcacgc cactgccctt tattgaaatc ataacctctt gtttggtctg
    11401 aatcatcaaa aatagtcgta gcattcacat aagaatcata ccaatgcccc agaaatttcc
    11461 ggcaaatctc tttcgcttta attgagtcat gatctacaga tgttatatat gataagcaat
    11521 ggtcgatatt atgaatatcg tgcccatatt cttgagccac ttcattataa agctcaagtt
    11581 gtgctttctt ttcgttagta tttataatcc aacttaatat catcggtagg ccaaattgag
    11641 cagcccactc agtcgtcgaa gctgattcag ccaccacata aaccggtgcg ccacctctgc
    11701 tatacgccgc ggggtttact tttaccttat ggaacttgat atgttcatta tcagcttcca
    11761 tatatccctc tgtcatgcca ttctttatca gcccgtacca gcattccgct aaggcgcgac
    11821 tgttattcat atctgtgccg aatacgcgaa agtccttgtt gtaaagccct cggcaaatac
    11881 caaaccgaaa tcgtcctttt gacatttgat ccaataaatt cacatcttca agttggcgta
    11941 ctggatgggc tgtgggaaga acaatagcgg cagttcctac attcaatttt ttagtcgcgc
    12001 caagtaaata tgcagcagcg acataagggt taccaagcaa accaaactcc gtgaaatgat
    12061 gctccagtaa ccatacggta tcaaaaccac actcctcaga gatgcgacct aatttaacca
    12121 aacgtttcat tacctctgtt tgagaaaatt ggggaggttg gtatgtaagc aaaaagtttc
    12181 caaatttcat agagagtcct tatattgcta tttgagtgat agaatatctc aatagatttt
    12241 aagacagaga aattgcttga ttttcaatct caattctcat tcggcgttca ttgactgtcg
    12301 caatagttaa atgttcaaat gacggttcag taatatcaac atcaatatcc agatgatcat
    12361 tatccatcgc gatagcggct ttcgtaaccg attgataaaa attgcgcagg accactaaat
    12421 tttcactcaa gtcatgcgaa cttcctaaca aagaatatat cttgcatcga ttactacgaa
    12481 tatttgataa caatgtgata acttcatctt gcttgaccca attatcgtta tttgcagtaa
    12541 aagcaataaa cggtatatca agatacatca tgttattaat tgtagaagct aaatcttccc
    12601 aaccaaaatc aagacaatct ctcgcaaaga cttcagcacc caatttatgg ccttcaaaat
    12661 ctagattatt cggcaattca ttaatgggta gactgagata atcaaaccct aaagctcttt
    12721 caagagaata tcttaagtta acaacaccga ctgcggtgat taaaaacgaa gcattgattt
    12781 cagataggct tgcataagct atccgcgcag ataagcttga agccaacata ccgaagttat
    12841 ttatttttcg tgtagttaac caatcaacca ctgctaacaa gctctgcttt cctatagaca
    12901 ttgtaaattc atcaattgtc cctgaactca atccaacgtg gtgaagcgaa tcatagcgga
    12961 tcacatgaaa tccattccgc gataaatatt ccgccagacc agcaaaatga tccatcctgc
    13021 gggcaaaacc agacgcaata ataatggcat tctttctctt tgggctgttt tcttctggca
    13081 gcgtttccca aacatgaatt tttttatttc cttcaacaca aataacgtgg tcgatggttt
    13141 tatattttga ttcattttcc atacttttta cctattatgg gacaaataca aggaacttat
    13201 cttcttccag gaatcgagtc tgttctattt caaccgcaac atccttagcc gtatagttag
    13261 atggcctttc atgagaaata tatgtcacta atcgttgcaa cggtctcatt ccgtcatgag
    13321 atccaccaac tcgaaatatg ttattcattc ctgcttctac aatcctttcc gcacctttta
    13381 atgctaacgc atctcgatat ttaaatgatg actcccaagg aaaaatagat atggtttgcg
    13441 tcttattttt ttgaacataa ggcaatattt gctcaatatt atcgacgtga tgaaggtaca
    13501 cacatctgcc aagtggttga ttaaattcca cacctgcatt tgactcaata atcatccaac
    13561 gttgatgaat atccacctct acttttaatc cagcaaacaa gctttctttt tgaactaaag
    13621 aataggccgc cttttcatca aaatcttttt tggcattcgg taatatatgc gcatatagat
    13681 taagtttttc tatcaacgct aacttaaatt cctcataatg atttcccatg taatatatgt
    13741 tttgggcaga aaaacaagct cgctgatcgt aaaaacaaac atcatgagcc gcacctgtcg
    13801 ctgcggacgt caaatcaaca ggattatcga taatgcaaag actcttttta gaaccaaatt
    13861 taatcacatc agcataagat ggcgcatgct ctaccgccca attaatcgca tctggccctc
    13921 cccaagcgac aataacatcc gcatgttgca taatttcttt tgcgagtgat gtatcacctt
    13981 ggtggggcca atatataaca gataaagagc gcgttatcgg atgattaggg tctacatcaa
    14041 taaaacttaa cgctaatgca ttagcggtaa aaggatcggt tgacgatgtt tttataatac
    14101 actgattctt agttaaaatt gcgcgtaata tagacatgat cccagataat ggaacattac
    14161 ctgccaacag atgtacagat ttacctttcg gaaaagcccg aacataactt tcatcctgag
    14221 gtagccattc atccatgata tggcgagaac caagttcatt ttctacaaca tcataaaggc
    14281 cgcctttaga acataaaatc atagatatcc aattggcctc tagcttagcc atttcttctg
    14341 aatatcccat atattttttt aagtcacgaa tgtatgtcct gcgtcttgag tattcttcat
    14401 ttttccatct ttgccctacc gtatagagaa aattgacaat gttatgcaac cgtaattcgt
    14461 tatttccatt acaatcaata atgtttttta catgagagtc attcaatatt ggcaggtaaa
    14521 cactattatc accaaaatta atggattgca ctaaatcatc accttcggga aagatttcaa
    14581 cctggccgtt aataatgaat gaaatttttt tagtcatacg tatcctccaa gcctgaattc
    14641 gtttaaacgg atccctcgag tggcattgaa cgctcccgtc ggcccatagg tcttgcaaaa
    14701 aaaatcggcg agttccgcgt ggtcggaagc ttccagctca ttttcccagc atattttcca
    14761 tcgcacttca gaagacatgc aaaagctcca agttgtttcg tcgcccaacc acgatcagag
    14821 ctgcgcgata ttgttgcgca tacttgttgg attgccggtt aggcaatcga gcggttaaga
    14881 gatgtaagag tctcaaatcg ggcccctgcc cggcgcttcg ttttttagtt cctggtcctg
    14941 atattgatca agttccgggt tttctataag tgatccgaaa gattggtaaa attgattgtt
    15001 tggatggcaa tcatctatgg aatggatatt caacccatgc gttttaaagg cctagatcta
    15061 aaccttcttg tagcactcga cgctctaatg accgagcgca agctcacagc agcggcgcgc
    15121 agcatcaacc tcagccaacc cgccatgagc gctgccatct ctaggttgcg cgactatttc
    15181 cgcgacgacc tttttatcat gcagagacgg gagctagttc cgaccccgcg tgcagaggca
    15241 cttgcccccg ctgttcgcga agcactcttg catattcagc tctccgttat cgcttgggat
    15301 ccaataaacc cagcggagtc cgaccgccga ttcagaatta tcctctcaga cttcatggca
    15361 ctggtcttct tcgacaagat aatactgcgc ttagctcggg aggcgccagg agtcagcttc
    15421 gagttgctgc cacttgacga cgatccggag gagcttctcc gccgtggtga tgtcgatttt
    15481 ctgatcctcc cggacttatt catgtccggc gcccatccga aggcaaggct tttcgaagag
    15541 agactggtgt gcgtcggctg ccctacgaac gagcagttac aagggcagct ctccttggag
    15601 caatatatgt ccatgggaca tgttgcggct aagttcggac gtggtctcaa gccttccgta
    15661 gagcaatggc tattgatgca gcacggcctc aagaggcgta ttgagctcgt cgtcccgggg
    15721 tttaacttga tcccgccatt gctgtctggc actaatcgaa tagcaaccat ccccctgcgg
    15781 ctggtcaaac attacgaacg aactatccca ctaagaatca ttgagcaggt acccccaccg
    15841 cggactctga ctacaaccgt gcgcaaggcg cattagggag gatgtatgta tgtaatcgcc
    15901 tgcggcatcg ttgccggctt ggcggctgcg gtggccctgt tgggcttcac gccgatgatg
    15961 gaggcgcttg ccgccggcga acgccgcaag gcactcgcgc aatggacgcg gacgatgttc
    16021 ctggtgctgc tgcctgtcgt gctgatgtgc gcgcccatcg ggtccagcat ttacgacgcc
    16081 gtgcaagcgg acgctggcaa gcccatcgct ttccacaacg gccggatcac ggtcgtcatg
    16141 gccctggtcg gcagcttggc cgttgtcctg gtcgcggctg cgcgtgcggt ggtcaaccgc
    16201 aagcatgcca gcttctggtt cgtcggctgg gtgatggcgt cggttttggc cggcggcgtc
    16261 ggcgcgatcg ccagcgcgaa gcaactggcg ttcctcggcg aacatagcgg catggtggcc
    16321 ttcggcttct tccgcgacca ggtgaaggac atgcactgcg atgcggacgt gatcctggcc
    16381 cggtgggatg aaaaggcgaa ctcgccggtg gtctaccgct gcccgaaggc gtacctgctc
    16441 aacaggttcg catccgcgcc cttcgtgccc tggccggact acaccgaggg ggaaagcgag
    16501 gatctaggta gggcgctcgc agcggccctg cgggacgcga aaaggtgaga aaagccgggc
    16561 actgcccggc tttatttttg ctgctgcgcg ttccaggccg cccacactcg tttgacctgg
    16621 ctcgggctgc atccgaccag cttggccgtc ttggcaatgc tcgatccgcc ggagcgaagc
    16681 gtgatgatgc ggtcgtgcat gccggcgtca cgtttgcggc cggtgtagcg gccggcggcc
    16741 ttcgccaact ggacaccctg acgttgacgc tcgcgccgat cctcgtagtc gtcgcgggcc
    16801 atctgcaagg cgagcttcaa aagcatgtcc tggacggatt ccagaacgat tttcgccact
    16861 ccgttcgcct cggcggccag ctccgacagg tccaccacgc caggcacggc cagcttggcc
    16921 cctttggccc ggatcgacgc aaccaggcgc tcggcctcgg ccaacggcaa gcggctgatg
    16981 cggtcgatct tctccgcaac gacgacttca ccaggttgca ggtccgcgat catgcgcagc
    17041 agctcgggcc ggtcggcgcg tgcgccggac gccttctcgc ggtagatgcc ggcgacgtag
    17101 tacccggcgg cccgcgtggc cgctacaagg ctctcctggc gttcaagatt ctgctcgtcc
    17161 gtactggcgc gcaggtagat gcgggcgacc ttcaaccttc gtccctccgg ttgttgctct
    17221 cgcgtcgcca tttccacggc tcgacggcgt gcggatcgga ccagaggccg acgcgcttgc
    17281 ctcgcgcctc ctgttcgagc cgcagcattt cagggtcggc cgcgcggccg tggaagcgat
    17341 aggcccacgc catgccctgg tgaaccatcg cggcgttgac gttgcgcggc tgcggcggcc
    17401 ggctggccag c
//
