LOCUS       sequence-173632-       17259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C4-dicarboxylates biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17259)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB714
FEATURES             Location/Qualifiers
     source          1..17259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3717..3733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3717..3733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3730..3752)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3741..3757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3765..3771,3772..3789,3790..3795))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3810..3831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3956..3974)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            4019..4127
                     /note="incP origin of transfer"
     promoter        4304..4408
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4409..4477,4478..5269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4627..4646)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(5796..6995)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             7101..7751
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      8241..8952
                     /label=oriV
                     /note="incP origin of replication"
     CDS             9619..10767
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10819..11931)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(12110..13093)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(13108..14190)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(14239..15162)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(15175..16617)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 tggcggcggg cacgaaggct gccgtcatct atgtcatcga caaggacggc atggcggttt
       61 cggccagtaa ctggcgcgaa ccgacgagct tcgtcggcaa tgactatcgt ttccgcgagt
      121 attttcaggg ggcggtcgaa cgaggacagg ccgagcactt cgcgctcggc acggtcagca
      181 agaaaccggg tctctatatc tcccagcgga tttcggacgg caatagcttg ctgggcgtcg
      241 ttgtggtcaa ggtcgaattc gacgacgtcg aggcggattg gaatgcctcg aacaccccgt
      301 cctatgtcgt tgacgagcgc ggcattgtcc tcatcaccag tattccgtca tggcggttca
      361 tgacgatcgg ccggatcgcc gaggaccggc tgacggcgat ccgcgaaagc cttcaaggta
      421 cccccaccgc ggactctgac tacaaccgtg cgcaaggcgc attagggagg atgtatgtat
      481 gtaatcgcct gcggcatcgt tgccggcttg gcggctgcgg tggccctgtt gggcttcacg
      541 ccgatgatgg aggcgcttgc cgccggcgaa cgccgcaagg cactcgcgca atggacgcgg
      601 acgatgttcc tggtgctgct gcctgtcgtg ctgatgtgcg cgcccatcgg gtccagcatt
      661 tacgacgccg tgcaagcgga cgctggcaag cccatcgctt tccacaacgg ccggatcacg
      721 gtcgtcatgg ccctggtcgg cagcttggcc gttgtcctgg tcgcggctgc gcgtgcggtg
      781 gtcaaccgca agcatgccag cttctggttc gtcggctggg tgatggcgtc ggttttggcc
      841 ggcggcgtcg gcgcgatcgc cagcgcgaag caactggcgt tcctcggcga acatagcggc
      901 atggtggcct tcggcttctt ccgcgaccag gtgaaggaca tgcactgcga tgcggacgtg
      961 atcctggccc ggtgggatga aaaggcgaac tcgccggtgg tctaccgctg cccgaaggcg
     1021 tacctgctca acaggttcgc atccgcgccc ttcgtgccct ggccggacta caccgagggg
     1081 gaaagcgagg atctaggtag ggcgctcgca gcggccctgc gggacgcgaa aaggtgagaa
     1141 aagccgggca ctgcccggct ttatttttgc tgctgcgcgt tccaggccgc ccacactcgt
     1201 ttgacctggc tcgggctgca tccgaccagc ttggccgtct tggcaatgct cgatccgccg
     1261 gagcgaagcg tgatgatgcg gtcgtgcatg ccggcgtcac gtttgcggcc ggtgtagcgg
     1321 ccggcggcct tcgccaactg gacaccctga cgttgacgct cgcgccgatc ctcgtagtcg
     1381 tcgcgggcca tctgcaaggc gagcttcaaa agcatgtcct ggacggattc cagaacgatt
     1441 ttcgccactc cgttcgcctc ggcggccagc tccgacaggt ccaccacgcc aggcacggcc
     1501 agcttggccc ctttggcccg gatcgacgca accaggcgct cggcctcggc caacggcaag
     1561 cggctgatgc ggtcgatctt ctccgcaacg acgacttcac caggttgcag gtccgcgatc
     1621 atgcgcagca gctcgggccg gtcggcgcgt gcgccggacg ccttctcgcg gtagatgccg
     1681 gcgacgtagt acccggcggc ccgcgtggcc gctacaaggc tctcctggcg ttcaagattc
     1741 tgctcgtccg tactggcgcg caggtagatg cgggcgacct tcaaccttcg tccctccggt
     1801 tgttgctctc gcgtcgccat ttccacggct cgacggcgtg cggatcggac cagaggccga
     1861 cgcgcttgcc tcgcgcctcc tgttcgagcc gcagcatttc agggtcggcc gcgcggccgt
     1921 ggaagcgata ggcccacgcc atgccctggt gaaccatcgc ggcgttgacg ttgcgcggct
     1981 gcggcggccg gctggccagc tccatgttga cccacacggt gcccagcgtg cggccgtaac
     2041 ggtcggtgtc cttctcgtcg accaggacgt gccggcggaa caccatgccg gccagcgcct
     2101 ggcgcgcacg ttcgccgaag gcttgccgct tttccggcgc gtcaatgtcc accaggcgca
     2161 cgcgcaccgg ctgcttgtct accagcacgt cgatggtgtc gccgtcgatg atgcgcacga
     2221 cctcgccgcg cagctcggcc catgccggcg aggcaacgac caggacggcc agcgcggcag
     2281 cggcgcgcag catggcgtag cttcggcgct tcatgcgtgg ccccattgct gatgatcggg
     2341 gtacgccagg tgcagcactg catcgaaatt ggccttgcag tagccgtcca gcgccacccg
     2401 cgagccgaac gccggcgaaa ggtactcgac caggccgggc cggtcgcgga cctcgcgccc
     2461 caggacgtgg atgcgccggc cgcgtgtgcc gtcgggtcca ggcacgaagg ccagcgcctc
     2521 gatgttgaag tcgatggata gaagttgtcg gtagtgcttg gccgccctca tcgcgtcccc
     2581 cttggtcaaa ttgggtatac ccatttgggc ctagtctagc cggcatggcg cattacagca
     2641 atacgcaatt taaatgcgcc tagcgcattt tcccgacctt aatgcgcctc gcgctgtagc
     2701 ctcacgccca catatgtgct aatgtggtta cgtgtatttt atggaggtta tccaatgagc
     2761 cgcctgacaa tcgacatgac ggaccagcag caccagagcc tgaaagccct ggccgccttg
     2821 cagggcaaga ccattaagca atacgccctc gaacgtctgt tccccggtga cgctgatgcc
     2881 gatcaggcat ggcaggaact gaaaaccatg ctggggaacc gcatcaacga tgggcttgcc
     2941 ggcaaggtgt ccaccaagag cgtcggcgaa attcttgatg aagaactcag cggggatcgc
     3001 gcttgacggc ctacatcctc acggctgagg ccgaagccga tctacgcggc atcatccgct
     3061 acacgcgccg ggagtggggc gcggcgcagg tgcgccgcta tatcgctaag ctggaacagg
     3121 gcatagccag gcttgccgcc ggcgaaggcc cgtttaagga catgagcgaa ctctttcccg
     3181 cgctgcggat ggcccgctgc gaacaccact acgttttttg cctgccgcgt gcgggcgaac
     3241 ccgcgttggt cgtggcgatc ctgcatgagc gcatggacct catgacgcga cttgccgaca
     3301 ggctcaaggg ctgatttcag ccgctaaaaa tcgcgccact cacaacgtcc tgatggcgta
     3361 cttacccaaa gaacagctag gagaatcatt tatgctcagc acacttccac aagctcatgc
     3421 aactttcttg aaccgcatcc gcgatgcggt cgcttccgat gttcgcttcc gcgctcttct
     3481 gatcggcggc tcttacgttc acggaggact cgatgagcac tccgatttgg atttcgacat
     3541 cgttgttgag gacaactgct acgcagatgt cttgtctaca cgcaaggatt ttgccgaggc
     3601 actgcccggc ttcctcaacg cgttcaccgg cgaacatgta ggagaaccgc gccttctgat
     3661 ctgcctatat ggtccgccac tgctacacat cgggggatca gcttggcgta atcatggtca
     3721 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3781 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3841 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3901 caacgcgcgg ggagaggcgg tttgcgtatt ggaattaatt cttgaagacg aaagggcctc
     3961 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gagcttatcg
     4021 gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga
     4081 agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt
     4141 ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa
     4201 aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa
     4261 agatccgtcg atcgacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4321 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4381 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4441 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4501 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4561 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4621 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     4681 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4741 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4801 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4861 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4921 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4981 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5041 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5101 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5161 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5221 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5281 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5341 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5401 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5461 ggggatcagg accgctgccg gagcgcaacc cactcactac agcagagcca tgtagacaac
     5521 atcccctccc cctttccacc gcgtcagacg cccgtagcag cccgctacgg gctttttcat
     5581 gccctgccct agcgtccaag cctcacggcc gcgctcggcc tctctggcgg ccttctggcg
     5641 ctcctgctgc ggcgtccgct cgtgggccgt ggcgcgggtc cgcgcgccgg cctcgtgcgc
     5701 ctggcgctcg cgggcgaggt ccagggcggc cgtcttcacg ttctgccttg cgcagatgag
     5761 atagatccgt caattccggc ctatcgtttc cacgatcagc gatcggctcg ttgccctgcg
     5821 ccgctccaaa gcccgcgacg cagcgccggc aggcagagca agtagagggc agcgcctgca
     5881 atccatgccc acccgttcca cgttgttata gaagccgcat agatcgccgt gaagaggagg
     5941 ggtccgacga tcgaggtcag gctggtgagc gccgccagtg agccttgcag ctgcccctga
     6001 cgttcctcat ccacctgcct ggacaacatt gcttgcagcg ccggcattcc gatgccaccc
     6061 gaagcaagca ggaccatgat cgggaacgcc atccatcccc gtgtcgcgaa ggcaagcagg
     6121 atgtagcctg tgccgtcggc aatcattccg agcatgagtg cccgcctttc gccgagccgg
     6181 gcggctacag ggccggtgat cattgcctgg gcgagtgaat gcagaatgcc aaatgcggca
     6241 agcgaaatgc cgatcgtggt cgcgtcccag tgaaagcgat cctcgccgaa aatgacccaa
     6301 agcgcggccg gcacctgtcc gacaagttgc atgatgaaga agaccgccat cagggcggcg
     6361 acgacggtca tgccccgggc ccaccggaac gaagcgagcg ggttgagagc ctcccggcgt
     6421 aacggccggc gttcgccttt gtgcgactcc ggcaaaagga aacagcccgt caggaaattg
     6481 aggccgttca aggctgccgc ggcgaagaac ggagcgtggg gggagaaacc gcccatcagc
     6541 ccaccgagca caggtcccgc gaccatcccg aacccgaaac aggcgctcat gaagccgaag
     6601 tgccgcgcgc gctcatcgcc atcagtgata tcggcaatat aagcgccggc taccgcccca
     6661 gtcgccccgg tgatgccggc cacgatccgc ccgatataga gaacccaaag gaaaggcgct
     6721 gtcgccatga tggcgtagtc gacagtggcg ccggccagcg agacgagcaa gattggccgc
     6781 cgcccgaaac gatccgacag cgcgcccagc acaggtgcgc aggcaaattg caccaacgca
     6841 tacagcgcca gcagaatgcc atagtgggcg gtgacgtcgt tcgagtgaac cagatcgcgc
     6901 aggaggcccg gcagcaccgg cataatcagg ccgatgccga cagcgtcgag cgcgacagtg
     6961 ctcagaatta cgatcagggg tatgttgggt ttcacgtctg gcctccggac cagcctccgc
     7021 tggtccgatt gaacgcgcgg attctttatc actgataagt tggtggacat attatgttta
     7081 tcagtgataa agtgtcaagc atgacaaagt tgcagccgaa tacagtgatc cgtgccgccc
     7141 tggacctgtt gaacgaggtc ggcgtagacg gtctgacgac acgcaaactg gcggaacggt
     7201 tgggggttca gcagccggcg ctttactggc acttcaggaa caagcgggcg ctgctcgacg
     7261 cactggccga agccatgctg gcggagaatc atacgcattc ggtgccgaga gccgacgacg
     7321 actggcgctc atttctgatc gggaatgccc gcagcttcag gcaggcgctg ctcgcctacc
     7381 gcgatggcgc gcgcatccat gccggcacgc gaccgggcgc accgcagatg gaaacggccg
     7441 acgcgcagct tcgcttcctc tgcgaggcgg gtttttcggc cggggacgcc gtcaatgcgc
     7501 tgatgacaat cagctacttc actgttgggg ccgtgcttga ggagcaggcc ggcgacagcg
     7561 atgccggcga gcgcggcggc accgttgaac aggctccgct ctcgccgctg ttgcgggccg
     7621 cgatagacgc cttcgacgaa gccggtccgg acgcagcgtt cgagcaggga ctcgcggtga
     7681 ttgtcgatgg attggcgaaa aggaggctcg ttgtcaggaa cgttgaagga ccgagaaagg
     7741 gtgacgattg atcaggaccg ctgccggagc gcaacccact cactacagca gagccatgta
     7801 gacaacatcc cctccccctt tccaccgcgt cagacgcccg tagcagcccg ctacgggctt
     7861 tttcatgccc tgccctagcg tccaagcctc acggccgcgc tcggcctctc tggcggcctt
     7921 ctggcgctcc tgctgcggcg tccgctcgtg ggccgtggcg cgggtccgcg cgccggcctc
     7981 gtgcgcctgg cgctcgcggg cgaggtccag ggcggccgtc ttcacgttct gccttgcgca
     8041 gatgagatag atcccggaat tgacggatct agcgtggact caaggctctc gcgaatggct
     8101 cgcgttggaa actttcattg acacttgagg ggcaccgcag ggaaattctc gtccttgcga
     8161 gaaccggcta tgtcgtgctg cgcatcgagc ctgcgccctt ggcttgtctc gcccctctcc
     8221 gcgtcgctac ggggcttcca gcgcctttcc gacgctcacc gggctggttg ccctcgccgc
     8281 tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc
     8341 gagacaccgc ggccgccggc gttgtggata cctcgcggaa aacttggccc tcactgacag
     8401 atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt tgacagatga
     8461 ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa
     8521 cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga taagtgccct
     8581 gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat ccttgacact
     8641 tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg ggctgtccac
     8701 aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc gctaacctgt
     8761 cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg gctgcgccct
     8821 gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc tcccggcccg
     8881 ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc
     8941 caaaaatggc agcgctggca gtccttgcca ttgccgggat cggggcagta acgggatggg
     9001 cgatcagccc gagcgcgacg cccggaagca ttgacgtgcc gcaggtgctg gcatcgacat
     9061 tcagcgacca ggtgccgggc agtgagggcg gcggcctggg tggcggcctg cccttcactt
     9121 cggccgtcgg ggcattcacg gacttcatgg cggggccggc aatttttacc ttgggcattc
     9181 ttggcatagt ggtcgcgggt gccgtgctcg tgttcggggg tgaattaatt ccccggatcg
     9241 atccgtcagc ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa aggaagcgga
     9301 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     9361 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     9421 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     9481 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     9541 caaggatctg atggcgcagg ggatcaagat cgacggatcg atccggggaa ttccggggca
     9601 atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg
     9661 atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt
     9721 gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc
     9781 gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag
     9841 cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg
     9901 cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc
     9961 agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag
    10021 ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg
    10081 gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac
    10141 aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg
    10201 gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc
    10261 ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat
    10321 ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc
    10381 ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc
    10441 ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg
    10501 ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg
    10561 acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg
    10621 gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag
    10681 gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat
    10741 gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca
    10801 gcagccacct gcaggtcatc aactatcaaa cgcttcggtt aagcttaaag cacacccttt
    10861 ctgcgtcctc gtattgacgc gacgtaaaat ttcaacgagc acgccgggat acttaccata
    10921 ttctctgcta attatcccga catcatcggt aacaataaat gctggataac tggttgctga
    10981 cgcatccata taactcatca accccggcgt tccatcaggt acaggtttca acgtttcagg
    11041 atcaagcgct cgcgcatata cccacggcgg aacatgttta cgctgcattt catcctcaaa
    11101 gaaacaagtg ttgagttcaa cttgattaaa tatatctcgg atctgactaa tatcactgag
    11161 attgaaagta tcaaataaaa gatgattgaa atcatcacgt ttcagagatt ctttttcgta
    11221 acttttccag ccgcctccgg ttatgatata aaggctttta tctccagaaa atgagatttt
    11281 tttatctttc atataatggc agagtaaata aataaagtat ggcgaaccaa taagacaaag
    11341 atctttccct tgatttttta ttcgttcaag actattcaat gttttaacaa aatctattcg
    11401 ttcttctgtt acggtaaatg tcgtaggata taacaattcc accaaactca taacatattt
    11461 aaaccaaata ttatgagcat taaatctatc tggtcccaaa ttgactaatt ctatttgatg
    11521 atcaaaccaa ctaccaacat atttcatgcc ataactcaca gagcctaaga gtctctcaat
    11581 acttaatctg tcacgcgcca cctgactttt taaaccattc gtgccgctac tggtaaacca
    11641 actttcaatc tcgttttcct gagaagttaa taagcgagta aacttaaaaa ccgatgttgg
    11701 gaatacaggt atgtcatcaa tttccgtaat attgtcatct actttgtgtg cctgacagta
    11761 gtgacgatat tctcgacaat gtttataatg attacgaaat gcatcaagca caagtttctt
    11821 tctgattttt tcctgctcgt cgtaagacca cactaatgga tcgctcgaaa aaatcaaatc
    11881 atcaatttct gagcttgctg taatttcttg tttatcaaca tatgaagtca tacctgtttt
    11941 actcctcaag ataatattag aaagtatggc agcactgctg tcatactctt ttataccctt
    12001 catctttcaa gctgctgctt tgttggctgc tttcactcac cccagtcaca tagttatcta
    12061 tgctcctggg gattcgttca cttgccgcct cgctgcaact cgaaatctat taggtatatt
    12121 ccatgtggta cttcttaata ttatcatcaa caatattgat tacatttttt tggctcatca
    12181 aatcattcat tggttcaaag gacagcaata cacttttcgc accacacttt tcaattgcca
    12241 acttagccgc agttatacac tccgtataat ttccgacagc gttttctgca attatttctt
    12301 caagtttatt ttcgaaattt tcattagggt gcatttcaag aacataatca ctaataaatg
    12361 cacgcgtctc ttgtttagct ttattactat cttcgttata gttaactaat atcattaact
    12421 gatggtctat ctctgatagg tcaacgtcat atttatccgc aacggcttta tatctttcag
    12481 catattcata tctaacatca ttagaatcat cccacttaaa gatgagagga ataccttttt
    12541 tggccgccca ctcaacaata tgatgactgg ttgctgttac atatttccga ggtccgcctg
    12601 gcgtataagc atggggattt acagatattt tagggaagct ataaaaatcg ttatctggat
    12661 tacaatagcc tgttgttaaa gcatcgttaa tgatttcata acactcttca aatagttgct
    12721 gttgatattc aaccgggcga ttaaaaaaat gcatttcatc ttttttttcg caatcactaa
    12781 accctaaaat aaatctccct tcacttaact gatccaataa gcaagcttcc tccgctatgc
    12841 ggacaggatg atgagttgta atgatgtgat ttaatgaacc aattttaatt ttctctgtta
    12901 aaccgagcag aaaaccagaa acagtcagag gagcgccgac aacaccatta tctgaaaaat
    12961 gattttcata cactaaaatc tgttcaaaat tcaacttatc aacatactcc gttatttcct
    13021 gcatgcgaac tatactttgt tcttgaacag ttgttgaatt gatgaagtta aggaagaaca
    13081 atccaaattt catttctttc tccttagcta atataatagc gaacgttgtt tttctttaag
    13141 aaatggcatg acatcagact ggaagagctt catggaagca ataatttcgt ctactgttcc
    13201 attagcttca aatccacaac aaatatttga tattcctgta gcatcaatgt ctttttgaat
    13261 tatgtcaata cattcctgcg gcgttcccac gggattgatt tcgtaactgt aatcaatacg
    13321 gcgattagta tctttatgtc cttttaatac aaagtcacgc cactgccctt tattgaaatc
    13381 ataacctctt gtttggtctg aatcatcaaa aatagtcgta gcattcacat aagaatcata
    13441 ccaatgcccc agaaatttcc ggcaaatctc tttcgcttta attgagtcat gatctacaga
    13501 tgttatatat gataagcaat ggtcgatatt atgaatatcg tgcccatatt cttgagccac
    13561 ttcattataa agctcaagtt gtgctttctt ttcgttagta tttataatcc aacttaatat
    13621 catcggtagg ccaaattgag cagcccactc agtcgtcgaa gctgattcag ccaccacata
    13681 aaccggtgcg ccacctctgc tatacgccgc ggggtttact tttaccttat ggaacttgat
    13741 atgttcatta tcagcttcca tatatccctc tgtcatgcca ttctttatca gcccgtacca
    13801 gcattccgct aaggcgcgac tgttattcat atctgtgccg aatacgcgaa agtccttgtt
    13861 gtaaagccct cggcaaatac caaaccgaaa tcgtcctttt gacatttgat ccaataaatt
    13921 cacatcttca agttggcgta ctggatgggc tgtgggaaga acaatagcgg cagttcctac
    13981 attcaatttt ttagtcgcgc caagtaaata tgcagcagcg acataagggt taccaagcaa
    14041 accaaactcc gtgaaatgat gctccagtaa ccatacggta tcaaaaccac actcctcaga
    14101 gatgcgacct aatttaacca aacgtttcat tacctctgtt tgagaaaatt ggggaggttg
    14161 gtatgtaagc aaaaagtttc caaatttcat agagagtcct tatattgcta tttgagtgat
    14221 agaatatctc aatagatttt aagacagaga aattgcttga ttttcaatct caattctcat
    14281 tcggcgttca ttgactgtcg caatagttaa atgttcaaat gacggttcag taatatcaac
    14341 atcaatatcc agatgatcat tatccatcgc gatagcggct ttcgtaaccg attgataaaa
    14401 attgcgcagg accactaaat tttcactcaa gtcatgcgaa cttcctaaca aagaatatat
    14461 cttgcatcga ttactacgaa tatttgataa caatgtgata acttcatctt gcttgaccca
    14521 attatcgtta tttgcagtaa aagcaataaa cggtatatca agatacatca tgttattaat
    14581 tgtagaagct aaatcttccc aaccaaaatc aagacaatct ctcgcaaaga cttcagcacc
    14641 caatttatgg ccttcaaaat ctagattatt cggcaattca ttaatgggta gactgagata
    14701 atcaaaccct aaagctcttt caagagaata tcttaagtta acaacaccga ctgcggtgat
    14761 taaaaacgaa gcattgattt cagataggct tgcataagct atccgcgcag ataagcttga
    14821 agccaacata ccgaagttat ttatttttcg tgtagttaac caatcaacca ctgctaacaa
    14881 gctctgcttt cctatagaca ttgtaaattc atcaattgtc cctgaactca atccaacgtg
    14941 gtgaagcgaa tcatagcgga tcacatgaaa tccattccgc gataaatatt ccgccagacc
    15001 agcaaaatga tccatcctgc gggcaaaacc agacgcaata ataatggcat tctttctctt
    15061 tgggctgttt tcttctggca gcgtttccca aacatgaatt tttttatttc cttcaacaca
    15121 aataacgtgg tcgatggttt tatattttga ttcattttcc atacttttta cctattatgg
    15181 gacaaataca aggaacttat cttcttccag gaatcgagtc tgttctattt caaccgcaac
    15241 atccttagcc gtatagttag atggcctttc atgagaaata tatgtcacta atcgttgcaa
    15301 cggtctcatt ccgtcatgag atccaccaac tcgaaatatg ttattcattc ctgcttctac
    15361 aatcctttcc gcacctttta atgctaacgc atctcgatat ttaaatgatg actcccaagg
    15421 aaaaatagat atggtttgcg tcttattttt ttgaacataa ggcaatattt gctcaatatt
    15481 atcgacgtga tgaaggtaca cacatctgcc aagtggttga ttaaattcca cacctgcatt
    15541 tgactcaata atcatccaac gttgatgaat atccacctct acttttaatc cagcaaacaa
    15601 gctttctttt tgaactaaag aataggccgc cttttcatca aaatcttttt tggcattcgg
    15661 taatatatgc gcatatagat taagtttttc tatcaacgct aacttaaatt cctcataatg
    15721 atttcccatg taatatatgt tttgggcaga aaaacaagct cgctgatcgt aaaaacaaac
    15781 atcatgagcc gcacctgtcg ctgcggacgt caaatcaaca ggattatcga taatgcaaag
    15841 actcttttta gaaccaaatt taatcacatc agcataagat ggcgcatgct ctaccgccca
    15901 attaatcgca tctggccctc cccaagcgac aataacatcc gcatgttgca taatttcttt
    15961 tgcgagtgat gtatcacctt ggtggggcca atatataaca gataaagagc gcgttatcgg
    16021 atgattaggg tctacatcaa taaaacttaa cgctaatgca ttagcggtaa aaggatcggt
    16081 tgacgatgtt tttataatac actgattctt agttaaaatt gcgcgtaata tagacatgat
    16141 cccagataat ggaacattac ctgccaacag atgtacagat ttacctttcg gaaaagcccg
    16201 aacataactt tcatcctgag gtagccattc atccatgata tggcgagaac caagttcatt
    16261 ttctacaaca tcataaaggc cgcctttaga acataaaatc atagatatcc aattggcctc
    16321 tagcttagcc atttcttctg aatatcccat atattttttt aagtcacgaa tgtatgtcct
    16381 gcgtcttgag tattcttcat ttttccatct ttgccctacc gtatagagaa aattgacaat
    16441 gttatgcaac cgtaattcgt tatttccatt acaatcaata atgtttttta catgagagtc
    16501 attcaatatt ggcaggtaaa cactattatc accaaaatta atggattgca ctaaatcatc
    16561 accttcggga aagatttcaa cctggccgtt aataatgaat gaaatttttt tagtcatacg
    16621 tatcctccaa gcctgaattc gtttaaacgg atccttgccc ttgctgtctg cgactgcatc
    16681 gagtggtgct gcgatcatga tgtcctccta gtggctcgtc cggaacgtac tccggcctcc
    16741 cgagccgttc gcttcaagtc caacagctca gccgttgttg ccaggttctt cgcaatcgcc
    16801 gtgccagttt tgcgtcgact agcaacgtat tgaattaatt acataaaaat ttacgaggct
    16861 tctgatatct cattaagaaa tgcggaaatc cgcacaaatc tatttgcgtt tcgtgcagaa
    16921 aaatgcacaa atccgccatg tccgtgtcgc agaaattgtg gccttccctg cccctgcaac
    16981 accgcatccg ccggatgtgg tgggcctacg ccgtgctcgc cctcctcgct gtcgtcgcaa
    17041 gcctgtgggc cagcggcgag atcggccggc accgagcgga ggctgctctc caggaacagg
    17101 cccgcatgga tgcgaggctg aatgcggctt tgctgcgcac ggttctcgaa aaataccggg
    17161 cgcttccctt cgtgctgtcg caggacgcgg cactcgctgc cgcactggcg ggaaacgatg
    17221 ccggcgcgtt cgagcggctc agtcagaagc tggaaaatt
//
