LOCUS       sequence-17364-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, 
            Buchberg AM, Largman C
  TITLE     AbdB-like Hox proteins stabilize DNA binding by the Meis1 
            homeodomain proteins.
  JOURNAL   Mol Cell Biol 1997 Nov;17(11):6448-58.
  PUBMED    9343407
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1997-11"; volume: "17(11)"; pages: "6448-58"
COMMENT     Sequence Label: HOXD12 (murine) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             110..142
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tgagaaattc cntctgaata ttttgtttac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggctagcat
      121 gactggtgga cagcaaatgg gtcgcggatc catgtgtgag cgcagtctct acagagctgg
      181 ctatgtgggc tcgcttctga atttacagtc accggactct ttctactttt ccaacctgag
      241 agccaatggc agccagttgg ccgcgcttcc ccccatctca taccctcgca gcgcgctgcc
      301 ctgggctact acgcccgcct catgcacccc tgcgcagcct gccaccgcct ctgcctttgg
      361 aggcttctct cagccttact tgaccggctc tgggccaatt ggcctgcagt ctccaggcgc
      421 caaggacgga cccgaagacc aggtcaagtt ctatacgcct gatgcgccca ccgcatctga
      481 ggaacgcagc cggactaggc cgcccttcgc ccccgagtct agtctggttc attcggctct
      541 caaaggcacc aagtatgact acgcgggtgt gggccggacc gctccaggct ctgcgaccct
      601 gctccagggg gccccctgtg cctccagctt caaggaagac accaaaggcc cgctcaactt
      661 gaacatggca gtgcaagtgg ccggggtggc ctcttgcctg cgatcttcac tgcccgacgg
      721 cctgccgtgg ggggcggccc cggggaaggg cccgcaagaa gaggaaaccc tacacaaagc
      781 agcagattgc ggagctggag aacgaattcc tggtcaatga attcatcaac cggcagaagc
      841 gtaaggaatt gtctaacagg ctgaacccnt ccgaaaangc aggtcaaaat ctggttcnan
      901 aaccggcgta tgaaaaaaan cgggtantgc ancgcgaanc aacactggcc ttttttnact
      961 tgg
//
