LOCUS       sequence-173650-        8311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HPV8 E6 K142A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Meyers JM, Uberoi A, Grace M, Lambert PF, Munger K
  TITLE     Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-beta 
            Tumor Suppressors to Inhibit Differentiation and Sustain 
            Keratinocyte Proliferation.
  JOURNAL   PLoS Pathog. 2017 Jan 20;13(1):e1006171. doi: 
            10.1371/journal.ppat.1006171.
  PUBMED    28107544
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2017-01-20"; volume: "13(1):e1006171. doi"; pages: 
            " 10.1371/journal.ppat.1006171"
COMMENT     Sequence Label: pLentiN HPV8 E6 K142A
FEATURES             Location/Qualifiers
     source          1..8311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..24
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             37..63
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(557..581)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             634..675
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    699..1287
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(752..772)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1170..1181)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        1302..1618
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1469..1604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1666..1713
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1732..2130
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             2217..2450
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2522..2643
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2559..2578)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2613..2632
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2683..2818
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2838..2857)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2839..2857)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2867..2884)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2867..2883)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2876..2898)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3025..3480
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3112..3131)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3322..3343
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3506..3610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3611..3679,3680..4471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3829..3848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4642..5230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5131..5150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5384..5401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5518..5539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5554..5559,5560..5577,5578..5584)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5592..5608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5597..5619
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5616..5632
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5616..5632
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5651..5671
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5653..5671
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5699..5925
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5926..6106
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6153..6278
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6771..7004
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7189..7233
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7500..7617
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7640..7943
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7944..8147
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8098..8118
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8144..8168
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8264..8288
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gactacaagg atgacgatga caagctcgat ggaggatacc catacgatgt tccagattac
       61 gctgatatcg gatccgacgg gcaggacaag gcttcatatt tagacactaa taaggacgag
      121 ctaccctcta ctattaaaga gttagctgcg gctttaggta ttccattgca ggactgttta
      181 gtaccgtgca acttttgtgg taactttttg gatttcttag aactgtgtga gtttgacaaa
      241 aagagactgt gcctaatttg gaaaaattac gttgttactg cgtgttgtcg ttgttgttgt
      301 gtagcaaccg caacgtttga atttaatgaa tattatgagc aaactgtgct aggcagagat
      361 attgaattag ctacaggacg ttcaattttt gagatagacg ttaggtgtca aaactgcttg
      421 tcatttttgg atatcataga gaaattagat tgctgtggga gaggccgtcc ctttcataaa
      481 gttagaggag gctgggcagg agtttgcagg ctttgtaagc atttgtatca tgattggtaa
      541 aagggtgggc gcgccgaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc
      601 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
      661 ggtctcgatt ctacgcgtac cggttagtaa tgatcgacaa tcaacctctg gattacaaaa
      721 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
      781 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
      841 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
      901 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
      961 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     1021 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     1081 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     1141 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     1201 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     1261 ggatctccct ttgggccgcc tccccgcctg gcgatggtac cggtgtggaa agtccccagg
     1321 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     1381 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1441 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1501 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     1561 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     1621 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     1681 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     1741 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     1801 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     1861 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     1921 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     1981 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     2041 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     2101 ctgccctctg gttatgtgtg ggagggctaa gcacaattcg agctcggtac ctttaagacc
     2161 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     2221 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     2281 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2341 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     2401 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     2461 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     2521 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     2581 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     2641 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     2701 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2761 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2821 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     2881 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2941 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3001 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3061 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3121 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3181 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3241 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3301 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3361 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3421 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     3481 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3541 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3601 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3661 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3721 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3781 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3841 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3901 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3961 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4021 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4081 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4141 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4201 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4261 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4321 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4381 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4441 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4501 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4561 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5281 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5341 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5401 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     5461 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     5521 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5581 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     5641 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     5701 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     5761 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     5821 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     5881 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     5941 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6001 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6061 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     6121 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     6181 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     6241 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     6301 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     6361 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     6421 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     6481 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     6541 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     6601 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     6661 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     6721 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     6781 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     6841 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     6901 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     6961 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     7021 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     7081 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     7141 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     7201 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     7261 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     7321 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     7381 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     7441 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     7501 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     7561 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
     7621 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     7681 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     7741 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     7801 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     7861 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     7921 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     7981 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     8041 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     8101 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     8161 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct
     8221 agaggatcca ctagtccagt gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa
     8281 agcaggctcc gcggccgccc ccttcaccat g
//
