LOCUS       sequence-173655-       10158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for Maml1 with LHHLL mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Meyers JM, Uberoi A, Grace M, Lambert PF, Munger K
  TITLE     Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-beta 
            Tumor Suppressors to Inhibit Differentiation and Sustain 
            Keratinocyte Proliferation.
  JOURNAL   PLoS Pathog. 2017 Jan 20;13(1):e1006171. doi: 
            10.1371/journal.ppat.1006171.
  PUBMED    28107544
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2017-01-20"; volume: "13(1):e1006171. doi"; pages: 
            " 10.1371/journal.ppat.1006171"
COMMENT     Sequence Label: pFLAG-CMV2 MAML LHHLL
FEATURES             Location/Qualifiers
     source          1..10158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..24
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5565..6187
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5764..5783)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6211..6231)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6216..6545
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6396..6531
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6583..6602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6584..6602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6616..6632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6616..6632)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6629..6651)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6640..6656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6664..6670,6671..6688,6689..6694))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6709..6730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6847..6864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7018..7606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7098..7117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7777..8568,8569..8637))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8400..8419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8638..8742)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8769..9224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8856..8875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9066..9087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9350..9372
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9364..9381
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9365..9381
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        9542..9921
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9922..10125
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10076..10096
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gactacaaag acgatgacga caagcttaag tcgactctag aggatcccgg gcccggcccg
       61 tgcagcccgc ggcccatggt gctgcccacc tgccccatgg cggagttcgc gctgccgcgg
      121 cacagcgcgg tcatggagcg ccttcgccgg cgcatcgagc tgtgccggcg ccaccacagc
      181 acctgcgagg cccgctacga ggccgtgtcg cccgagcgcc tggagctgga gcgccaacac
      241 accttcgccc tgcaccagcg ctgcatccag gccaaggcca agcgcgccgg gaagcacagg
      301 cagccgcccg ccgccacggc cccggcgccc gccgccccgg ccccgcgcct ggacgccgct
      361 gacggccccg agcacggccg cccggccacg catcttcatg atacagttaa gaggaatctt
      421 gacagcgcca cttcccctca gaatggcgat caacagaatg gctacgggga cctctttcct
      481 gggcataaga agactcgccg ggaggcccct ctgggagttg ccatctcttc caatggactg
      541 cctccagcct cccccctctg tcagtctgac aagccttctg gagccgacgc cctgcagtcc
      601 agtgggaagc actctctggg gctagactct ctcaacaaaa agcgtctggc tgactccagc
      661 cttcacttga atggaggcag taaccccagt gagtcatttc ctctgagcct gaataaagaa
      721 ctgaagcagg agcctgtcga agacctgcct tgcatgatca ctgggactgt cggctccata
      781 tcgcaaagca acctcatgcc agacctcaac cttaacgagc aggagtggaa ggagctcatc
      841 gaggagctga acaggtcggt gcccgatgaa gacatgaagg acctgtttaa tgaggacttc
      901 gaggagaaga aggacccaga gtcttctggc tctgccacac aaaccccctt ggcacaggac
      961 attaatatta agacggaatt ctctccagca gcctttgagc aagaacagtt aggctctcca
     1021 caagtgaggg ccgggtctgc agggcagacc tttctggggc cttcctctgc ccctgtgagt
     1081 acagattccc ccagcctagg gggctcccaa accttattcc acacctctgg tcagccccgg
     1141 gcggacaatc ccagtccaaa cctgatgccg gcatcagccc aggcccagaa cgcacaaaga
     1201 gcccttgcag gtgtggtatt gcccagtcag ggcccaggag gggcctcaga gctgtcctct
     1261 gcccaccagc tccagcagat cgctgccaag cagaagcgcg agcagatgct ccagaaccca
     1321 cagcaggcca ccccggcacc agccccgggc cagatgtcca catggcagca gacggggccc
     1381 tcccacagtt ccttagatgt cccttacccc atggagaagc ctgccagccc ttccagctac
     1441 aagcaagact tcactaactc caaactgctc atgatgccta gtgtgaataa gagttcccct
     1501 cggcccggag gcccctacct ccagcccagc catgtgaacc tgctgagtca ccagccaccg
     1561 agtaacttga atcagaactc cgcgaataac caggggtctg tgctggacta cggcaataca
     1621 aaaccccttt ctcattacaa agcggactgt gggcaaggca gcccggggtc tggccagagc
     1681 aagccagccc tgatggctta tcttccccag cagctgtccc atataagtca cgagcagaac
     1741 tccctgtttc tgatgaagcc aaagccagga aatatgcctt tccgatcact ggttccacct
     1801 ggccaggagc agaacccttc cagtgtccct gtgcaagccc aggctaccag tgttgggacc
     1861 cagccgcctg ccgtgtccgt ggccagctcc cacaacagct ccccctatct cagcagccag
     1921 caacaggccg ctgtaatgaa gcagcatcag ttgcttttgg accaacagaa acaaagggag
     1981 cagcagcaaa agcatttaca gcaacagcag ttccttcaga ggcaacagca ccttctcgcg
     2041 gaacaggaga agcaacagtt tcagcgccat ctgacccgcc caccacccca gtaccaagac
     2101 ccgacacaag gcagcttccc acagcaggtt ggacagttca cagggtcctc tgctgccgtg
     2161 cccggcatga acaccttggg tccatccaac tccagctgtc ctcgagtgtt ccctcaggct
     2221 gggaatctga tgccaatggg ccctggacat gcttcagttt cctctctccc cacaaactca
     2281 ggccaacagg accggggtgt ggctcagttc cctggctccc aaaacatgcc tcagagcagc
     2341 ctctatggca tggcttctgg cataacccag atagttgccc agcccccgcc acaggccacc
     2401 aatggacatg cccacattcc acggcagacc aacgtgggcc agaacacctc cgtctcagct
     2461 gcctatgggc agaactctct gggaagctct ggcctctccc agcagcacaa taaggggacc
     2521 ctgaaccctg gtttaacaaa gccaccggtc ccaagggtgt caccagccat gggaggccag
     2581 aattcctcct ggcagcatca gggaatgccg aacctcagtg gccagacccc agggaacagc
     2641 aacgtgagtc ccttcactgc agcctccagt ttccacatgc agcagcaggc ccacctgaaa
     2701 atgtctagcc cgcaattctc ccaggcagtg cccaacaggc ccatggctcc catgagctca
     2761 gcagctgccg tggggtcctt gctaccccca gtgagtgcac agcagaggac cagcgcccct
     2821 gccccagcac cacccccaac agcccctcag cagggcttgc ctggcctgag cccagcaggg
     2881 cctgagctgg gggccttcag ccagagccct gcctcacaga tgggcggtcg ggcggggctg
     2941 cactgcaccc aggcctaccc tgtgcggacc gcgggccagg agctgccttt tgcctatagc
     3001 gggcagccag gtggcagtgg gctctctagt gtggctggac acaccgatct gatcgactcc
     3061 ctgctgaaga acaggacttc agaggagtgg atgagtgatt tgcaccacct gttagggtct
     3121 cagtaatgga aggatttgta gtgtttttag tgttcattca tcctatattt ttattctcag
     3181 attcaaagaa agagcaacta ctttggacca aaagcccatg gcctggggag ctgggcaggt
     3241 agagcccaag ctccaggtga ggcctggccc tgggcagggt ctgtggctgc gcccctcagg
     3301 ccagcagttg aggtccatcg ggctggcccc agcccatctg ctggcatcag tacctggtgt
     3361 tgggacagca ggatagggtt ctaaaggtgg ttttctatcc aaacgaccaa aaaaccaaca
     3421 gtaacaccag tgaaacccca cactgtcggg cttataaaaa tctgtgccat catggtgatt
     3481 ttatccaaga ctgctccact taccctagtg ctggggacaa gtttctgttg aaactttaga
     3541 tagcagaatt atttgcaatt tgtagcatag aaaagatttt taaatttttt tacaaaaggt
     3601 ttttaaacag attagggtag gtgatggttt aaatcaatta agtggcattg gaaacctagg
     3661 gtttcctttt gattaagagc cttttttgtt tctgctcttt gtcagctttc aggggagaag
     3721 gaggccactg gaaaattatt tccctaagtg caggctgttg actgcgtatg ccaaaaaggg
     3781 acaggaggca tgggatagca ggtctggtga cacagctagg gtcttcctag cagctcctcc
     3841 tcctccctcc caaggccccc aggaatccct tcctcccatg tcctggcagc aggaccccag
     3901 gctacatatg gaaggtagag atgtgggggt cctgtgtcct ggagtattat gtctccccac
     3961 cttctgcagt tttctctgaa catgtatgtt gcccatggtg ggagcgtggt cactgtgcag
     4021 ttgtgcacag atgtctttcc tttaccgttg gcctttctgt ctgcctctcc ttcctctctg
     4081 cagcccaaat ggaaaacaat tatttactcc attggaggga aaggaagagt cttagaattc
     4141 ctaagggaac cttagcataa aggttttggg gaaggaggcc gtaggccggc ccggaggaag
     4201 caattccact tggtttgaca acttctgcca ctcccatgtc agatgacttg cacttcttaa
     4261 agagattgct ttataacact aagacatcct ttctaaagat tcaagtggac ttgactaagc
     4321 tgagggtcca cgaaatagaa tatgacatgt gagctgtttt tggaaaacga agatggagag
     4381 agcacttccc cgtaacgaaa gcaaagtggt aagcacaggg tgagaccctt ttacacagaa
     4441 tggtggagag aaaagagaat gctgaaaagt ggctcagatg cagagtgttc tgtggagaaa
     4501 ctgcagcccc acttctgttt ccctggagtc tcccaatgga tcattcagga gtgtcctatg
     4561 tgagaattga gccaaggaaa atactcatgc aaccagcctg agtcgcggtg aggggacgag
     4621 aggttgtaca cacattggta gttattttgc accagcagtg cctttctcac tgggggtact
     4681 tggaccctca gatcttcttt tctaatagcc atttgccacc ccaagtggta tgtcggccat
     4741 ttctccttaa aacaccttcc ctacctttcc catgtactca gtttagctct caaagaaggg
     4801 gtgaatcata aagccagtga aaatttcacc ctctgaggga gttccccaat ctgaagggga
     4861 agagggtgac ctcagcggct tttctcccaa aaatcggctg aaggctggtt gtggatcctt
     4921 gttcctctcc tgaccccatc tggctgctgc cccatctccc acccctgtcc ccggggctcg
     4981 ctggccctgc actccgcctt agtcctgggg ccggcgacac agtgggggct cctcacttgc
     5041 tgcagtgtca tagcaataaa atgtgattct tggggtcccc ccagggagct gcccatggct
     5101 ttatttatga acctggtttt cgggagtcag gggaggagat gactttgctt ctgtgcacag
     5161 ccccgtcttc caggagccac gactcagaag aaaagggtgc tcagactttt gttatacaca
     5221 tttgctttgt gtaaataaat gtttacaatt ttatatgaaa gatggaataa gcgctagagc
     5281 ttccaactgt atatttttta cttttataga ttttaaaact atgatccttt atatgtgtgt
     5341 tttgggggag ctatgataag ttttatggca aacggttggt attgttaact ttttattgtc
     5401 atcaaaagtt cataaaagtc ctattaatcc ccatattctt ctactgccct taactctggt
     5461 atacaccaaa aagaaatctt tactttcctt gttttatcat tataaaaata aagtattttg
     5521 ctagtatgga aaaaaaaaaa aaaaaaaggg cggccgcgga tcccgggtgg catccctgtg
     5581 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     5641 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     5701 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     5761 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     5821 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     5881 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     5941 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     6001 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta aaataactat
     6061 accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa ccggtgggac
     6121 atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag tagatgcctg
     6181 ttgaattggg tacgcggcca gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6241 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6301 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6361 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6421 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6481 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6541 gcaaaaagct cctcgaggaa ctgaaaaacc agaaagttaa ttccctatag tgagtcgtat
     6601 taaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6661 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6721 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6781 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7681 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7741 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7801 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7861 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7921 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7981 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8041 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8101 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8161 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8221 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8281 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8341 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8401 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8461 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8521 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8581 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8641 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8701 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8761 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     8821 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     8881 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8941 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9001 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9061 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9121 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9181 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg
     9241 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9301 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     9361 cgttgtaaaa cgacggccag tgccaagctg atctatacat tgaatcaata ttggcaatta
     9421 gccatattag tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg
     9481 ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg accgccatgt
     9541 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     9601 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     9661 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     9721 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     9781 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
     9841 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
     9901 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
     9961 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
    10021 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
    10081 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
    10141 cagaattgat ctaccatg
//
