LOCUS       sequence-173658-       11985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11985)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 11985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-D1994A
FEATURES             Location/Qualifiers
     source          1..11985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        81..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     539..559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        634..652
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      684..693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             693..722
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             738..767
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    786..810
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8427..8451)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8500..8519)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8501..8519)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8793..8914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8830..8849)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8884..8903
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8921..9376)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9058..9079)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9270..9289
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9403..9507
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9509..9866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9509..9700
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9532..9552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9717..9852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9901..10695
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9901..10695
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9955..9974)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10565..10584
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10883..10902)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10927..10974
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11303..11891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11792..11811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
      601 cgtcagatcc gctagcgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa
      661 gctggagctc caccgcggtg gcggccgcca tggagcagaa actcatctct gaagaggatc
      721 tgagcccggg cggatccgag cagaaactca tctctgaaga ggatctgctg caggaattcg
      781 atatcacaag tttgtacaaa aaagcaggct ccgaattcgc ccttatggct agtggcagct
      841 gtcaggggtg cgaagaggac gaggaaactc tgaagaagtt gatagtcagg ctgaacaatg
      901 tccaggaagg aaaacagata gaaacgctgg tccaaatcct ggaggatctg ctggtgttca
      961 cgtactccga gcacgcctcc aagttatttc aaggcaaaaa tatccatgtg cctctgttga
     1021 tcgtcttgga ctcctatatg agagtcgcga gtgtgcagca ggtgggttgg tcacttctgt
     1081 gcaaattaat agaagtctgt ccaggtacaa tgcaaagctt aatgggaccc caggatgttg
     1141 gaaatgattg ggaagtcctt ggtgttcacc aattgattct taaaatgcta acagttcata
     1201 atgccagtgt aaacttgtca gtgattggac tgaagacctt agatctcctc ctaacttcag
     1261 gtaaaatcac cttgctgata ttggatgaag aaagtgatat tttcatgtta atttttgatg
     1321 ccatgcactc atttccagcc aatgatgaag tccagaaact tggatgcaaa gctttacatg
     1381 tgctgtttga gagagtctca gaggagcaac tgactgaatt tgttgagaac aaagattata
     1441 tgatattgtt aagtgcgtta acaaatttta aagatgaaga ggaaattgtg cttcatgtgc
     1501 tgcattgttt acattcccta gcgattcctt gcaataatgt ggaagtcctc atgagtggca
     1561 atgtcaggtg ttataatatt gtggtggaag ctatgaaagc attccctatg agtgaaagaa
     1621 ttcaagaagt gagttgctgt ttgctccata ggcttacatt aggtaatttt ttcaatatcc
     1681 tggtattaaa cgaagtccat gagtttgtgg tgaaagctgt gcagcagtac ccagagaatg
     1741 cagcattgca gatctcagcg ctcagctgtt tggccctcct cactgagact attttcttaa
     1801 atcaagattt agaggaaaag aatgagaatc aagagaatga tgatgagggg gaagaagata
     1861 aattgttttg gctggaagcc tgttacaaag cattaacgtg gcatagaaag aacaagcacg
     1921 tgcaggaggc cgcatgctgg gcactaaata atctccttat gtaccaaaac agtttacatg
     1981 agaagattgg agatgaagat ggccatttcc cagctcatag ggaagtgatg ctctccatgc
     2041 tgatgcattc ttcatcaaag gaagttttcc aggcatctgc gaatgcattg tcaactctct
     2101 tagaacaaaa tgttaatttc agaaaaatac tgttatcaaa aggaatacac ctgaatgttt
     2161 tggagttaat gcagaagcat atacattctc ccgaagtggc tgaaagtggc tgtaaaatgc
     2221 taaatcatct ttttgaagga agcaacactt ccctggatat aatggcagca gtggtcccca
     2281 aaatactaac agttatgaaa cgtcatgaga catcattacc agtgcagctg gaggcgcttc
     2341 gagctatttt acattttata gtgcctggca tgccagaaga atccagggag gatacagaat
     2401 ttcatcataa gctaaatatg gttaaaaaac agtgtttcaa gaatgatatt cacaaactgg
     2461 tcctagcagc tttgaacagg ttcattggaa atcctgggat tcagaaatgt ggattaaaag
     2521 taatttcttc tattgtacat tttcctgatg cattagagat gttatccctg gaaggtgcta
     2581 tggattcagt gcttcacaca ctgcagatgt atccagatga ccaagaaatt cagtgtctgg
     2641 gtttaagtct tataggatac ttgattacaa agaagaatgt gttcatagga actggacatc
     2701 tgctggcaaa aattctggtt tccagcttat accgatttaa ggatgttgct gaaatacaga
     2761 ctaaaggatt tcagacaatc ttagcaatcc tcaaattgtc agcatctttt tctaagctgc
     2821 tggtgcatca ttcatttgac ttagtaatat tccatcaaat gtcttccaat atcatggaac
     2881 aaaaggatca acagtttcta aacctctgtt gcaagtgttt tgcaaaagta gctatggatg
     2941 attacttaaa aaatgtgatg ctagagagag cgtgtgatca gaataacagc atcatggttg
     3001 aatgcttgct tctattggga gcagatgcca atcaagcaaa ggagggatct tctttaattt
     3061 gtcaggtatg tgagaaagag agcagtccca aattggtgga actcttactg aatagtggat
     3121 ctcgtgaaca agatgtacga aaagcgttga cgataagcat tgggaaaggt gacagccaga
     3181 tcatcagctt gctcttaagg aggctggccc tggatgttgc caacaatagc atttgccttg
     3241 gaggattttg tataggaaaa gttgaacctt cttggcttgg tcctttattt ccagataaga
     3301 cttctaattt aaggaaacaa acaaatatag catctacact agcaagaatg gtgatcagat
     3361 atcagatgaa aagtgctgtg gaagaaggaa cagcctcagg cagcgatgga aatttttctg
     3421 aagatgtgct gtctaaattt gatgaatgga cctttattcc tgactcttct atggacagtg
     3481 tgtttgctca aagtgatgac ctggatagtg aaggaagtga aggctcattt cttgtgaaaa
     3541 agaaatctaa ttcaattagt gtaggagaat tttaccgaga tgccgtatta cagcgttgct
     3601 caccaaattt gcaaagacat tccaattcct tggggcccat ttttgatcat gaagatttac
     3661 tgaagcgaaa aagaaaaata ttatcttcag atgattcact caggtcatca aaacttcaat
     3721 cccatatgag gcattcagac agcatttctt ctctggcttc tgagagagaa tatattacat
     3781 cactagacct ttcagcaaat gaactaagag atattgatgc cctaagccag aaatgctgta
     3841 taagtgttca tttggagcat cttgaaaagc tggagcttca ccagaatgca ctcacgagct
     3901 ttccacaaca gctatgtgaa actctgaaga gtttgacaca tttggacttg cacagtaata
     3961 aatttacatc atttccttct tatttgttga aaatgagttg tattgctaat cttgatgtct
     4021 ctcgaaatga cattggaccc tcagtggttt tagatcctac agtgaaatgt ccaactctga
     4081 aacagtttaa cctgtcatat aaccagctgt cttttgtacc tgagaacctc actgatgtgg
     4141 tagagaaact ggagcagctc attttagaag gaaataaaat atcagggata tgctccccct
     4201 tgagactgaa ggaactgaag attttaaacc ttagtaagaa ccacatttca tccctatcag
     4261 agaactttct tgaggcttgt cctaaagtgg agagtttcag tgccagaatg aattttcttg
     4321 ctgctatgcc tttcttgcct ccttctatga caatcctaaa attatctcag aacaaatttt
     4381 cctgtattcc agaagcaatt ttaaatcttc cacacttgcg gtctttagat atgagcagca
     4441 atgatattca gtacctacca ggtcccgcac actggaaatc tttgaactta agggaactct
     4501 tatttagcca taatcagatc agcatcttgg acttgagtga aaaagcatat ttatggtcta
     4561 gagtagagaa actgcatctt tctcacaata aactgaaaga gattcctcct gagattggct
     4621 gtcttgaaaa tctgacatct ctggatgtca gttacaactt ggaactaaga tcctttccca
     4681 atgaaatggg gaaattaagc aaaatatggg atcttccttt ggatgaactg catcttaact
     4741 ttgattttaa acatatagga tgtaaagcca aagacatcat aaggtttctt caacagcgat
     4801 taaaaaaggc tgtgccttat aaccgaatga aacttatgat tgtgggaaat actgggagtg
     4861 gtaaaaccac cttattgcag caattaatga aaaccaagaa atcagatctt ggaatgcaaa
     4921 gtgccacagt tggcatagat gtgaaagact ggcctatcca aataagagac aaaagaaaga
     4981 gagatctcgt cctaaatgtg tgggattttg caggtcgtga ggaattctat agtactcatc
     5041 cccattttat gacgcagcga gcattgtacc ttgctgtcta tgacctcagc aagggacagg
     5101 ctgaagttga tgccatgaag ccttggctct tcaatataaa ggctcgcgct tcttcttccc
     5161 ctgtgattct cgttggcaca catttggatg tttctgatga gaagcaacgc aaagcctgca
     5221 tgagtaaaat caccaaggaa ctcctgaata agcgagggtt ccctgccata cgagattacc
     5281 actttgtgaa tgccaccgag gaatctgatg ctttggcaaa acttcggaaa accatcataa
     5341 acgagagcct taatttcaag atccgagatc agcttgttgt tggacagctg attccagact
     5401 gctatgtaga acttgaaaaa atcattttat cggagcgtaa aaatgtgcca attgaatttc
     5461 ccgtaattga ccggaaacga ttattacaac tagtgagaga aaatcagctg cagttagatg
     5521 aaaatgagct tcctcacgca gttcactttc taaatgaatc aggagtcctt cttcattttc
     5581 aagacccagc actgcagtta agtgacttgt actttgtgga acccaagtgg ctttgtaaaa
     5641 tcatggcaca gattttgaca gtgaaagtgg aaggttgtcc aaaacaccct aagggaatta
     5701 tttcgcgtag agatgtggaa aaatttcttt caaaaaaaag gaaatttcca aagaactaca
     5761 tgtcacagta ttttaagctc ctagaaaaat tccagattgc tttgccaata ggagaagaat
     5821 atttgctggt tccaagcagt ttgtctgacc acaggcctgt gatagagctt ccccattgtg
     5881 agaactctga aattatcatc cgactatatg aaatgcctta ttttccaatg ggattttggt
     5941 caagattaat caatcgatta cttgagattt caccttacat gctttcaggg agagaacgag
     6001 cacttcgccc aaacagaatg tattggcgac aaggcattta cttaaattgg tctcctgaag
     6061 cttattgtct ggtaggatct gaagtcttag acaatcatcc agagagtttc ttaaaaatta
     6121 cagttccttc ttgtagaaaa ggctgtattc ttttgggcca agttgtggac cacattgatt
     6181 ctctcatgga agaatggttt cctgggttgc tggagattga tatttgtggt gaaggagaaa
     6241 ctctgttgaa gaaatgggca ttatatagtt ttaatgatgg cgaagaacat caaaaaatct
     6301 tacttgatga cttgatgaag aaagcagagg aaggagatct cttagtaaat ccagatcaac
     6361 caaggctcac cattccaata tctcagattg cccctgactt gattttggct gacctgccta
     6421 gaaatattat gttgaataat gatgagttgg aatttgaaca agctccagag tttctcctag
     6481 gtgatggcag ttttggatca gtttaccgag cagcctatga aggagaagaa gtggctgtga
     6541 agatttttaa taaacataca tcactcaggc tgttaagaca agagcttgtg gtgctttgcc
     6601 acctccacca ccccagtttg atatctttgc tggcagctgg gattcgtccc cggatgttgg
     6661 tgatggagtt agcctccaag ggttccttgg atcgcctgct tcagcaggac aaagccagcc
     6721 tcactagaac cctacagcac aggatcgcac tccacgtagc tgatggtttg agatacctcc
     6781 actcagccat gattatatac cgagccctga aaccccacaa tgtgctgctt ttcacactgt
     6841 atcccaatgc tgccatcatc gcaaagattg ctgactacgg cattgctcag tactgctgta
     6901 gaatggggat aaaaacatca gagggcacac cagggtttcg tgcacctgaa gttgccagag
     6961 gaaatgtcat ttataaccaa caggctgatg tttattcatt tggtttacta ctctatgaca
     7021 ttttgacaac tggaggtaga atagtagagg gtttgaagtt tccaaatgag tttgatgaat
     7081 tagaaataca aggaaaatta cctgatccag ttaaagaata tggttgtgcc ccatggccta
     7141 tggttgagaa attaattaaa cagtgtttga aagaaaatcc tcaagaaagg cctacttctg
     7201 cccaggtctt tgacattttg aattcagctg aattagtctg tctgacgaga cgcattttat
     7261 tacctaaaaa cgtaattgtt gaatgcatgg ttgctacaca tcacaacagc aggaatgcaa
     7321 gcatttggct gggctgtggg cacaccgaca gaggacagct ctcatttctt gacttaaata
     7381 ctgaaggata cacttctgag gaagttgctg atagtagaat attgtgctta gccttggtgc
     7441 atcttcctgt tgaaaaggaa agctggattg tgtctgggac acagtctggt actctcctgg
     7501 tcatcaatac cgaagatggg aaaaagagac ataccctaga aaagatgact gattctgtca
     7561 cttgtttgta ttgcaattcc ttttccaagc aaagcaaaca aaaaaatttt cttttggttg
     7621 gaaccgctga tggcaagtta gcaatttttg aagataagac tgttaagctt aaaggagctg
     7681 ctcctttgaa gatactaaat ataggaaatg tcagtactcc attgatgtgt ttgagtgaat
     7741 ccacaaattc aacggaaaga aatgtaatgt ggggaggatg tggcacaaag attttctcct
     7801 tttctaatga tttcaccatt cagaaactca ttgagacaag aacaagccaa ctgttttctt
     7861 atgcagcttt cagtgattcc aacatcataa cagtggtggt agacactgct ctctatattg
     7921 ctaagcaaaa tagccctgtt gtggaagtgt gggataagaa aactgaaaaa ctctgtggac
     7981 taatagactg cgtgcacttt ttaagggagg taatggtaaa agaaaacaag gaatcaaaac
     8041 acaaaatgtc ttattctggg agagtgaaaa ccctctgcct tcagaagaac actgctcttt
     8101 ggataggaac tggaggaggc catattttac tcctggatct ttcaactcgt cgacttatac
     8161 gtgtaattta caacttttgt aattcggtca gagtcatgat gacagcacag ctaggaagcc
     8221 ttaaaaatgt catgctggta ttgggctaca accggaaaaa tactgaaggt acacaaaagc
     8281 agaaagagat acaatcttgc ttgaccgttt gggacatcaa tcttccacat gaagtgcaaa
     8341 atttagaaaa acacattgaa gtgagaaaag aattagctga aaaaatgaga cgaacatctg
     8401 ttgagtaaga gagaagggcg aattcgaccc agctttcttg tacaaagtgg tgatcggtac
     8461 cttaattaat taaggtacca ggtaagtgta cccaattcgc cctatagtga gtcgtattac
     8521 aattcactcg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gagatccaat
     8581 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattcaca
     8641 gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc
     8701 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     8761 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     8821 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8881 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt
     8941 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     9001 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     9061 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     9121 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     9181 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     9241 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     9301 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     9361 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9481 cttcaataat attgaaaaag gaagaatcct gaggcggaaa gaaccagctg tggaatgtgt
     9541 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9601 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     9661 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     9721 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     9781 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     9841 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     9901 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     9961 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
    10021 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
    10081 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
    10141 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
    10201 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
    10261 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
    10321 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
    10381 gaacatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
    10441 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
    10501 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    10561 atagcgttgg ctacccgtga tattgctgaa gaacttggcg gcgaatgggc tgaccgcttc
    10621 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    10681 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
    10741 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    10801 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    10861 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
    10921 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
    10981 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
    11041 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
    11101 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
    11161 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11221 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11281 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    11341 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    11401 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    11461 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    11521 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11581 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11641 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11701 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11761 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11821 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11881 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11941 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggata
//
