LOCUS       sequence-173659-       11985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11985)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 11985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-G2019S
FEATURES             Location/Qualifiers
     source          1..11985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        401..604
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     555..575
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        650..668
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      700..709
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             709..738
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             754..783
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8443..8467)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8516..8535)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8517..8535)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8809..8930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8846..8865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8900..8919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8937..9392)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9074..9095)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9286..9305
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9419..9523
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9525..9882
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9525..9716
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9548..9568)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9733..9868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9917..10711
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9917..10711
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9971..9990)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10581..10600
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10899..10918)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10943..10990
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11319..11907
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11808..11827
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
       61 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      421 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      601 agctggttta gtgaaccgtc agatccgcta gcgattacgc caagctcgaa attaaccctc
      661 actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgccatgga gcagaaactc
      721 atctctgaag aggatctgag cccgggcgga tccgagcaga aactcatctc tgaagaggat
      781 ctgctgcagg aattcgatat cacaagtttg tacaaaaaag caggctccga attcgccctt
      841 atggctagtg gcagctgtca ggggtgcgaa gaggacgagg aaactctgaa gaagttgata
      901 gtcaggctga acaatgtcca ggaaggaaaa cagatagaaa cgctggtcca aatcctggag
      961 gatctgctgg tgttcacgta ctccgagcac gcctccaagt tatttcaagg caaaaatatc
     1021 catgtgcctc tgttgatcgt cttggactcc tatatgagag tcgcgagtgt gcagcaggtg
     1081 ggttggtcac ttctgtgcaa attaatagaa gtctgtccag gtacaatgca aagcttaatg
     1141 ggaccccagg atgttggaaa tgattgggaa gtccttggtg ttcaccaatt gattcttaaa
     1201 atgctaacag ttcataatgc cagtgtaaac ttgtcagtga ttggactgaa gaccttagat
     1261 ctcctcctaa cttcaggtaa aatcaccttg ctgatattgg atgaagaaag tgatattttc
     1321 atgttaattt ttgatgccat gcactcattt ccagccaatg atgaagtcca gaaacttgga
     1381 tgcaaagctt tacatgtgct gtttgagaga gtctcagagg agcaactgac tgaatttgtt
     1441 gagaacaaag attatatgat attgttaagt gcgttaacaa attttaaaga tgaagaggaa
     1501 attgtgcttc atgtgctgca ttgtttacat tccctagcga ttccttgcaa taatgtggaa
     1561 gtcctcatga gtggcaatgt caggtgttat aatattgtgg tggaagctat gaaagcattc
     1621 cctatgagtg aaagaattca agaagtgagt tgctgtttgc tccataggct tacattaggt
     1681 aattttttca atatcctggt attaaacgaa gtccatgagt ttgtggtgaa agctgtgcag
     1741 cagtacccag agaatgcagc attgcagatc tcagcgctca gctgtttggc cctcctcact
     1801 gagactattt tcttaaatca agatttagag gaaaagaatg agaatcaaga gaatgatgat
     1861 gagggggaag aagataaatt gttttggctg gaagcctgtt acaaagcatt aacgtggcat
     1921 agaaagaaca agcacgtgca ggaggccgca tgctgggcac taaataatct ccttatgtac
     1981 caaaacagtt tacatgagaa gattggagat gaagatggcc atttcccagc tcatagggaa
     2041 gtgatgctct ccatgctgat gcattcttca tcaaaggaag ttttccaggc atctgcgaat
     2101 gcattgtcaa ctctcttaga acaaaatgtt aatttcagaa aaatactgtt atcaaaagga
     2161 atacacctga atgttttgga gttaatgcag aagcatatac attctcccga agtggctgaa
     2221 agtggctgta aaatgctaaa tcatcttttt gaaggaagca acacttccct ggatataatg
     2281 gcagcagtgg tccccaaaat actaacagtt atgaaacgtc atgagacatc attaccagtg
     2341 cagctggagg cgcttcgagc tattttacat tttatagtgc ctggcatgcc agaagaatcc
     2401 agggaggata cagaatttca tcataagcta aatatggtta aaaaacagtg tttcaagaat
     2461 gatattcaca aactggtcct agcagctttg aacaggttca ttggaaatcc tgggattcag
     2521 aaatgtggat taaaagtaat ttcttctatt gtacattttc ctgatgcatt agagatgtta
     2581 tccctggaag gtgctatgga ttcagtgctt cacacactgc agatgtatcc agatgaccaa
     2641 gaaattcagt gtctgggttt aagtcttata ggatacttga ttacaaagaa gaatgtgttc
     2701 ataggaactg gacatctgct ggcaaaaatt ctggtttcca gcttataccg atttaaggat
     2761 gttgctgaaa tacagactaa aggatttcag acaatcttag caatcctcaa attgtcagca
     2821 tctttttcta agctgctggt gcatcattca tttgacttag taatattcca tcaaatgtct
     2881 tccaatatca tggaacaaaa ggatcaacag tttctaaacc tctgttgcaa gtgttttgca
     2941 aaagtagcta tggatgatta cttaaaaaat gtgatgctag agagagcgtg tgatcagaat
     3001 aacagcatca tggttgaatg cttgcttcta ttgggagcag atgccaatca agcaaaggag
     3061 ggatcttctt taatttgtca ggtatgtgag aaagagagca gtcccaaatt ggtggaactc
     3121 ttactgaata gtggatctcg tgaacaagat gtacgaaaag cgttgacgat aagcattggg
     3181 aaaggtgaca gccagatcat cagcttgctc ttaaggaggc tggccctgga tgttgccaac
     3241 aatagcattt gccttggagg attttgtata ggaaaagttg aaccttcttg gcttggtcct
     3301 ttatttccag ataagacttc taatttaagg aaacaaacaa atatagcatc tacactagca
     3361 agaatggtga tcagatatca gatgaaaagt gctgtggaag aaggaacagc ctcaggcagc
     3421 gatggaaatt tttctgaaga tgtgctgtct aaatttgatg aatggacctt tattcctgac
     3481 tcttctatgg acagtgtgtt tgctcaaagt gatgacctgg atagtgaagg aagtgaaggc
     3541 tcatttcttg tgaaaaagaa atctaattca attagtgtag gagaatttta ccgagatgcc
     3601 gtattacagc gttgctcacc aaatttgcaa agacattcca attccttggg gcccattttt
     3661 gatcatgaag atttactgaa gcgaaaaaga aaaatattat cttcagatga ttcactcagg
     3721 tcatcaaaac ttcaatccca tatgaggcat tcagacagca tttcttctct ggcttctgag
     3781 agagaatata ttacatcact agacctttca gcaaatgaac taagagatat tgatgcccta
     3841 agccagaaat gctgtataag tgttcatttg gagcatcttg aaaagctgga gcttcaccag
     3901 aatgcactca cgagctttcc acaacagcta tgtgaaactc tgaagagttt gacacatttg
     3961 gacttgcaca gtaataaatt tacatcattt ccttcttatt tgttgaaaat gagttgtatt
     4021 gctaatcttg atgtctctcg aaatgacatt ggaccctcag tggttttaga tcctacagtg
     4081 aaatgtccaa ctctgaaaca gtttaacctg tcatataacc agctgtcttt tgtacctgag
     4141 aacctcactg atgtggtaga gaaactggag cagctcattt tagaaggaaa taaaatatca
     4201 gggatatgct cccccttgag actgaaggaa ctgaagattt taaaccttag taagaaccac
     4261 atttcatccc tatcagagaa ctttcttgag gcttgtccta aagtggagag tttcagtgcc
     4321 agaatgaatt ttcttgctgc tatgcctttc ttgcctcctt ctatgacaat cctaaaatta
     4381 tctcagaaca aattttcctg tattccagaa gcaattttaa atcttccaca cttgcggtct
     4441 ttagatatga gcagcaatga tattcagtac ctaccaggtc ccgcacactg gaaatctttg
     4501 aacttaaggg aactcttatt tagccataat cagatcagca tcttggactt gagtgaaaaa
     4561 gcatatttat ggtctagagt agagaaactg catctttctc acaataaact gaaagagatt
     4621 cctcctgaga ttggctgtct tgaaaatctg acatctctgg atgtcagtta caacttggaa
     4681 ctaagatcct ttcccaatga aatggggaaa ttaagcaaaa tatgggatct tcctttggat
     4741 gaactgcatc ttaactttga ttttaaacat ataggatgta aagccaaaga catcataagg
     4801 tttcttcaac agcgattaaa aaaggctgtg ccttataacc gaatgaaact tatgattgtg
     4861 ggaaatactg ggagtggtaa aaccacctta ttgcagcaat taatgaaaac caagaaatca
     4921 gatcttggaa tgcaaagtgc cacagttggc atagatgtga aagactggcc tatccaaata
     4981 agagacaaaa gaaagagaga tctcgtccta aatgtgtggg attttgcagg tcgtgaggaa
     5041 ttctatagta ctcatcccca ttttatgacg cagcgagcat tgtaccttgc tgtctatgac
     5101 ctcagcaagg gacaggctga agttgatgcc atgaagcctt ggctcttcaa tataaaggct
     5161 cgcgcttctt cttcccctgt gattctcgtt ggcacacatt tggatgtttc tgatgagaag
     5221 caacgcaaag cctgcatgag taaaatcacc aaggaactcc tgaataagcg agggttccct
     5281 gccatacgag attaccactt tgtgaatgcc accgaggaat ctgatgcttt ggcaaaactt
     5341 cggaaaacca tcataaacga gagccttaat ttcaagatcc gagatcagct tgttgttgga
     5401 cagctgattc cagactgcta tgtagaactt gaaaaaatca ttttatcgga gcgtaaaaat
     5461 gtgccaattg aatttcccgt aattgaccgg aaacgattat tacaactagt gagagaaaat
     5521 cagctgcagt tagatgaaaa tgagcttcct cacgcagttc actttctaaa tgaatcagga
     5581 gtccttcttc attttcaaga cccagcactg cagttaagtg acttgtactt tgtggaaccc
     5641 aagtggcttt gtaaaatcat ggcacagatt ttgacagtga aagtggaagg ttgtccaaaa
     5701 caccctaagg gaattatttc gcgtagagat gtggaaaaat ttctttcaaa aaaaaggaaa
     5761 tttccaaaga actacatgtc acagtatttt aagctcctag aaaaattcca gattgctttg
     5821 ccaataggag aagaatattt gctggttcca agcagtttgt ctgaccacag gcctgtgata
     5881 gagcttcccc attgtgagaa ctctgaaatt atcatccgac tatatgaaat gccttatttt
     5941 ccaatgggat tttggtcaag attaatcaat cgattacttg agatttcacc ttacatgctt
     6001 tcagggagag aacgagcact tcgcccaaac agaatgtatt ggcgacaagg catttactta
     6061 aattggtctc ctgaagctta ttgtctggta ggatctgaag tcttagacaa tcatccagag
     6121 agtttcttaa aaattacagt tccttcttgt agaaaaggct gtattctttt gggccaagtt
     6181 gtggaccaca ttgattctct catggaagaa tggtttcctg ggttgctgga gattgatatt
     6241 tgtggtgaag gagaaactct gttgaagaaa tgggcattat atagttttaa tgatggcgaa
     6301 gaacatcaaa aaatcttact tgatgacttg atgaagaaag cagaggaagg agatctctta
     6361 gtaaatccag atcaaccaag gctcaccatt ccaatatctc agattgcccc tgacttgatt
     6421 ttggctgacc tgcctagaaa tattatgttg aataatgatg agttggaatt tgaacaagct
     6481 ccagagtttc tcctaggtga tggcagtttt ggatcagttt accgagcagc ctatgaagga
     6541 gaagaagtgg ctgtgaagat ttttaataaa catacatcac tcaggctgtt aagacaagag
     6601 cttgtggtgc tttgccacct ccaccacccc agtttgatat ctttgctggc agctgggatt
     6661 cgtccccgga tgttggtgat ggagttagcc tccaagggtt ccttggatcg cctgcttcag
     6721 caggacaaag ccagcctcac tagaacccta cagcacagga tcgcactcca cgtagctgat
     6781 ggtttgagat acctccactc agccatgatt atataccgag acctgaaacc ccacaatgtg
     6841 ctgcttttca cactgtatcc caatgctgcc atcatcgcaa agattgctga ctacagcatt
     6901 gctcagtact gctgtagaat ggggataaaa acatcagagg gcacaccagg gtttcgtgca
     6961 cctgaagttg ccagaggaaa tgtcatttat aaccaacagg ctgatgttta ttcatttggt
     7021 ttactactct atgacatttt gacaactgga ggtagaatag tagagggttt gaagtttcca
     7081 aatgagtttg atgaattaga aatacaagga aaattacctg atccagttaa agaatatggt
     7141 tgtgccccat ggcctatggt tgagaaatta attaaacagt gtttgaaaga aaatcctcaa
     7201 gaaaggccta cttctgccca ggtctttgac attttgaatt cagctgaatt agtctgtctg
     7261 acgagacgca ttttattacc taaaaacgta attgttgaat gcatggttgc tacacatcac
     7321 aacagcagga atgcaagcat ttggctgggc tgtgggcaca ccgacagagg acagctctca
     7381 tttcttgact taaatactga aggatacact tctgaggaag ttgctgatag tagaatattg
     7441 tgcttagcct tggtgcatct tcctgttgaa aaggaaagct ggattgtgtc tgggacacag
     7501 tctggtactc tcctggtcat caataccgaa gatgggaaaa agagacatac cctagaaaag
     7561 atgactgatt ctgtcacttg tttgtattgc aattcctttt ccaagcaaag caaacaaaaa
     7621 aattttcttt tggttggaac cgctgatggc aagttagcaa tttttgaaga taagactgtt
     7681 aagcttaaag gagctgctcc tttgaagata ctaaatatag gaaatgtcag tactccattg
     7741 atgtgtttga gtgaatccac aaattcaacg gaaagaaatg taatgtgggg aggatgtggc
     7801 acaaagattt tctccttttc taatgatttc accattcaga aactcattga gacaagaaca
     7861 agccaactgt tttcttatgc agctttcagt gattccaaca tcataacagt ggtggtagac
     7921 actgctctct atattgctaa gcaaaatagc cctgttgtgg aagtgtggga taagaaaact
     7981 gaaaaactct gtggactaat agactgcgtg cactttttaa gggaggtaat ggtaaaagaa
     8041 aacaaggaat caaaacacaa aatgtcttat tctgggagag tgaaaaccct ctgccttcag
     8101 aagaacactg ctctttggat aggaactgga ggaggccata ttttactcct ggatctttca
     8161 actcgtcgac ttatacgtgt aatttacaac ttttgtaatt cggtcagagt catgatgaca
     8221 gcacagctag gaagccttaa aaatgtcatg ctggtattgg gctacaaccg gaaaaatact
     8281 gaaggtacac aaaagcagaa agagatacaa tcttgcttga ccgtttggga catcaatctt
     8341 ccacatgaag tgcaaaattt agaaaaacac attgaagtga gaaaagaatt agctgaaaaa
     8401 atgagacgaa catctgttga gtaagagaga agggcgaatt cgacccagct ttcttgtaca
     8461 aagtggtgat cggtacctta attaattaag gtaccaggta agtgtaccca attcgcccta
     8521 tagtgagtcg tattacaatt cactcgatcg cccttcccaa cagttgcgca gcctgaatgg
     8581 cgaatggaga tccaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     8641 gtattttaga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca cagtctgttc
     8701 atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     8761 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     8821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     8941 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     9001 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     9061 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     9121 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     9181 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     9241 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     9301 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     9361 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     9421 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9481 ataaccctga taaatgcttc aataatattg aaaaaggaag aatcctgagg cggaaagaac
     9541 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     9601 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     9661 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     9721 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9781 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9841 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     9901 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     9961 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
    10021 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
    10081 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
    10141 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
    10201 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
    10261 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
    10321 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
    10381 ggatgatctg gacgaagaac atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
    10441 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
    10501 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
    10561 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagaac ttggcggcga
    10621 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
    10681 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
    10741 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
    10801 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    10861 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
    10921 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
    10981 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
    11041 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
    11101 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
    11161 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    11221 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    11281 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    11341 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    11401 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    11461 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    11521 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    11581 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    11641 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    11701 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    11761 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    11821 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    11881 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    11941 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcc
//
