LOCUS       sequence-17366-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  TITLE     HOX protein coding clones not referenced in publications
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HOXA1 (murine) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             94..126
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             546..563
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(616..634)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      630..677
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(801..820)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatggcta gcatgactgg tggacagcaa
      121 atgggtcgcg gatccgaatt caccatgaaa gttaaaagaa accctcccaa aacagggaaa
      181 gttggagagt acggctacgt gggtcaaccc aacgcagtgc gcaccaattt caccaccaag
      241 cagctcacag agctggagaa ggagttccac ttcaacaagt accttacacg agcgcgcagg
      301 gtggagattg ccgcgtccct acagctcaat gagacccagg tgaagatctg gttccagaat
      361 cgccgcatga agcagaagaa gcgtgagaag gaggggctcc tgcccatctc ccctgccact
      421 cctcctggca gcgatgagaa aacggaagaa tcatctgaga aatctagccc ctcgcccagt
      481 gccccttctc cggcatcgtc tacctcagac actctgacta cctcccactg aggcggatcc
      541 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      601 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      661 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
      721 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      781 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttcntc gcacgttcgc
      841 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      901 acggcacctc gaccccaaaa acttgattag ggtgatggtt cacg
//
