LOCUS       sequence-173663-        8266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MmuPV1 E6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Meyers JM, Uberoi A, Grace M, Lambert PF, Munger K
  TITLE     Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-beta 
            Tumor Suppressors to Inhibit Differentiation and Sustain 
            Keratinocyte Proliferation.
  JOURNAL   PLoS Pathog. 2017 Jan 20;13(1):e1006171. doi: 
            10.1371/journal.ppat.1006171.
  PUBMED    28107544
REFERENCE   2  (bases 1 to 8266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2017-01-20"; volume: "13(1):e1006171. doi"; pages: 
            " 10.1371/journal.ppat.1006171"
COMMENT     Sequence Label: pLentiN MmuPV1 E6
FEATURES             Location/Qualifiers
     source          1..8266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..24
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             37..63
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(512..536)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             589..630
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    654..1242
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(707..727)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1125..1136)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        1257..1573
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1424..1559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1621..1668
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1687..2085
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             2172..2405
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2477..2598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2514..2533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2568..2587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2638..2773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2793..2812)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2794..2812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2822..2839)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2822..2838)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2831..2853)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2980..3435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3067..3086)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3277..3298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3461..3565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3566..3634,3635..4426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3784..3803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4597..5185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5086..5105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5339..5356
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5473..5494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5509..5514,5515..5532,5533..5539)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5547..5563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5552..5574
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5571..5587
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5571..5587
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5606..5626
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5608..5626
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5654..5880
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5881..6061
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6108..6233
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6726..6959
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7144..7188
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7455..7572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7595..7898
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7899..8102
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8053..8073
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8099..8123
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8219..8243
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gactacaagg atgacgatga caagctcgat ggaggatacc catacgatgt tccagattac
       61 gctgatatcg gatccgaaat cggcaaaggc tacactctcg aggaggtgct tagatattct
      121 aacaaagatg tcgtggattt tcatttgtct tgtgcttttt gctctactac tatggatcat
      181 aacgagaagg ccagattcat acaggctaaa ttgaaatgtg ttgttagaga ttttgctttt
      241 aaaggtgctt gtattgtgtg ccgcagacag cttgcttgca aggaaaagct tttgcatact
      301 agagttacag gggaggctga tttggtagag tgcatggctg gcaagaatat tgtgtttgtt
      361 actgtaagat gtgttacgtg cctggcactc cttactgcct ctgaaaagct tgatgccaaa
      421 gcgtgcggct tgccatttca cttggtgcgc cacatgtgga gaggctactg cgggttctgc
      481 aaaccattac tataaaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggttgat
      541 atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc
      601 cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgatc gacaatcaac
      661 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
      721 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
      781 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
      841 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
      901 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
      961 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     1021 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     1081 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     1141 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     1201 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggcgat ggtaccggtg
     1261 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     1321 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     1381 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     1441 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     1501 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     1561 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     1621 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     1681 taaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     1741 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     1801 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     1861 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     1921 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     1981 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     2041 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagcaca attcgagctc
     2101 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     2161 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     2221 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2281 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2341 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2401 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     2461 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2521 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2581 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     2641 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     2701 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     2761 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     2821 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     2881 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     2941 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     3001 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3061 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3121 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3181 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3241 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3301 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3361 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3421 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3481 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3541 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3601 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3661 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3721 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3781 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3841 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3901 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3961 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4021 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4081 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4141 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4201 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4261 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4321 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4381 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4441 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4501 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4561 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4621 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4681 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4741 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4801 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4861 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4921 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4981 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5041 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5101 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5161 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5221 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5281 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5341 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     5401 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     5461 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     5521 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5581 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     5641 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     5701 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     5761 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     5821 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     5881 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5941 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6001 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6061 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     6121 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     6181 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     6241 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     6301 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     6361 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     6421 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     6481 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     6541 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     6601 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     6661 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     6721 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     6781 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     6841 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     6901 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     6961 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     7021 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     7081 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     7141 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     7201 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     7261 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     7321 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     7381 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     7441 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     7501 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     7561 attcaaaatt ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc
     7621 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     7681 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     7741 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     7801 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     7861 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     7921 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     7981 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     8041 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     8101 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     8161 gaagacaccg actctagagg atccactagt ccagtgtggt ggaattctgc agatatcaac
     8221 aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatg
//
