LOCUS       sequence-173677-        2601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression vector for producing 6x histidine tagged 
            dynamin 1a proline rich domain (residues 746-864).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2601)
  TITLE     DYRK1A assay plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHT-PRD
FEATURES             Location/Qualifiers
     source          1..2601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(406..424)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(484..502)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        570..674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(675..743,744..1535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(893..912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1706..2294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2195..2214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2448..2465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2546..2565
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2546..2564
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 catcatcatc atcatcataa cacgaccacc gtcagcacgc ccatgccccc gcccgtggac
       61 gactcctggc tgcaggtgca gagcgtaccg gccggacgca ggtcgcccac gtccagcccc
      121 acgccgcagc gccgagcccc cgccgtgccc ccagcccggc ccgggtcgcg gggccctgct
      181 cctgggcctc cgcctgctgg atccgcccta ggaggagctc ctcctgttcc atccagacca
      241 ggagctagcc ctgatccttt tggtcctcct cctcaagtac cttcgcgacc aaaccgcgct
      301 cctcctgggg taccaagtcg ttcaggacaa gcatcacctt ctagaccaga atctccacga
      361 ccacctcttg acctatgact cgagagcttg catctatggt ctctcccttt agtgagggtt
      421 aattagaaac tggagtcgat gataagctgt caaacatgag aattaattct tgaagacgaa
      481 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      541 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      901 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
      961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1501 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1561 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1621 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2341 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2401 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2461 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2521 attaatgcag ctggcttatc gaaattaata cgactcacta tagggagacc ggaattcaaa
     2581 attaaggagg atccatcgat g
//
