LOCUS       sequence-17369-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     HOX protein coding clones not referenced in publications
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HOXC9 (murine) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..70
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             74..106
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 aaggagatat accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc
       61 gcgcggcagc catatggcta gcatgactgg tggacagcaa atgggtcgcg gatccgaatt
      121 ccaccatgtc ggcgacgggg cccatcagta actattacgt ggactcgctc atctctcacg
      181 acaatgaaga cctcctagcg tccaggtttc cggccaccgg ggctcaccct gccgccgcca
      241 gacccagcgg cttggtgccg gactgtagcg attttccgtc ctgtagcttc gcgcccaagc
      301 cggctgtatt cagtacgtcg tgggcgccgg tgccctcgca gtcgtctgtg gtctatcacc
      361 cttacggccc ccagccccac ctcggcgccg acacgcacta catgcggact tggctcgagc
      421 cgctgtccgg cgccgtctcc ttccccagct tcccggccgg gggccgtcac tacgccctca
      481 agcccgacgc ctacccgggg cgccgcgccg actgcggccc gggcgacggc cgcagctacc
      541 cggactacat gtacggctcg cccggggaac tgcgcgaccg cgccccgcag acgctgccct
      601 cgcccgaggc ggacgcgctg gccggcagca agcacaaaga ggagaaggcc gacctggacc
      661 ctagcaaccc cgtggccaac tggatccacg cccgttccac aaggaagaag cgctgcccct
      721 acaccaagta ccagacgctg gaactg
//
