LOCUS       sequence-173705-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  antigen for mouse PRDM14 purification ( aa 1-231).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Tu S, Narendra V, Yamaji M, Vidal SE, Rojas LA, Wang X, Kim SY, 
            Garcia BA, Tuschl T, Stadtfeld M, Reinberg D
  TITLE     Co-repressor CBFA2T2 regulates pluripotency and germline 
            development.
  JOURNAL   Nature. 2016 Jun 8;534(7607):387-90. doi: 10.1038/nature18004.
  PUBMED    27281218
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-8- 8"; volume: "534"; issue: "7607"; pages:
            "387-90"
COMMENT     Sequence Label: mP14H1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             739..756
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(809..827)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      823..870
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tagantaatt ttgtttaact ttaagaagga gatatacata tggccttacc gcccnctnnn
       61 gagacccagt cccaggacaa ggcgaactac ctgccccaga gcaaccctca ccacctgact
      121 acctactacg cgcatgcccc aggctacagt cactttagga acctcgccac caccgaggag
      181 gagtttcaac cttggaaact ggcagctgca gtgcttgagt cccaggcgat ggcccctctt
      241 gatgcttttc ggatgacggc tcccttgttg aacccgggtc tggctgttca gagtgagccg
      301 ctctacaatc tgccctggta caaattgtca ccatggaacc gaattcctca attcactccc
      361 gaagtaccac gatttctaga cagcactgag cataggagca gcggttccag caaccaaaat
      421 ttggtcctcg gcggcggcgg tggccaaatc agtggtcaga ggtgggaagc tgaaaattta
      481 cttctgccat ccccggtaat tgcttcccta ctacctgatg gaattaagtc gtcccagtca
      541 atatctgtcc cccaaacctt gaatcaagag gggaagctgc cgttttgcgg cttcaacttc
      601 acagaggagg aactgagctt cgttctgtat ggagccatcg ctagtccgga gcacccaacc
      661 gacttacagc atgcaatttc aggcatcctg gttcccacag agagttctgg gtctaatcat
      721 cttcataaaa ccctcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      781 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      841 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
      901 aatggggacg cgccctgtag cggcgcatta
//
