LOCUS       sequence-173706-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  antigen for mouse PRDM14 purification ( aa 1-231).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Tu S, Narendra V, Yamaji M, Vidal SE, Rojas LA, Wang X, Kim SY, 
            Garcia BA, Tuschl T, Stadtfeld M, Reinberg D
  TITLE     Co-repressor CBFA2T2 regulates pluripotency and germline 
            development.
  JOURNAL   Nature. 2016 Jun 8;534(7607):387-90. doi: 10.1038/nature18004.
  PUBMED    27281218
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-8- 8"; volume: "534"; issue: "7607"; pages:
            "387-90"
COMMENT     Sequence Label: mP14H1
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             752..774
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    789..813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(813..832)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(814..832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agggtttnnn
       61 nnnangnnta gacccagaac tctctgtggg aaccaggatg cctgaaattg catgctgtaa
      121 gtcggttggg tgctccggac tagcgatggc tccatacaga acgaagctca gttcctcctc
      181 tgtgaagttg aagccgcaaa acggcagctt cccctcttga ttcaaggttt gggggacaga
      241 tattgactgg gacgacttaa ttccatcagg tagtagggaa gcaattaccg gggatggcag
      301 aagtaaattt tcagcttccc acctctgacc actgatttgg ccaccgccgc cgccgaggac
      361 caaattttgg ttgctggaac cgctgctcct atgctcagtg ctgtctagaa atcgtggtac
      421 ttcgggagtg aattgaggaa ttcggttcca tggtgacaat ttgtaccagg gcagattgta
      481 gagcggctca ctctgaacag ccagacccgg gttcaacaag ggagccgtca tccgaaaagc
      541 atcaagaggg gccatcgcct gggactcaag cactgcagct gccagtttcc aaggttgaaa
      601 ctcctcctcg gtggtggcga ggttcctaaa gtgactgtag cctggggcat gcgcgtagta
      661 ggtagtcagg tggtgagggt tgctctgggg caggtagttc gccttgtcct gggactgggt
      721 ctcaccagag ggcggtaagg ccatatgtat atctccttct taaagttaaa caaaattatt
      781 tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg
      841 ggatcgagat cgatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
      901 acaggtgcgg ttgctggcgc ctatatcgcc gac
//
