LOCUS       sequence-173716-        8188 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  disruption of human RMRP gene via CRISPR-Cas9, guide 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Goldfarb KC, Cech TR
  TITLE     Targeted CRISPR disruption reveals a role for RNase MRP RNA in human
            preribosomal RNA processing.
  JOURNAL   Genes Dev. 2017 Jan 1;31(1):59-71. doi: 10.1101/gad.286963.116. Epub
            2017 Jan 23.
  PUBMED    28115465
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.286963.116"; journalName: "Genes Dev"; date: 
            "2017-01-1- 1"; volume: "31"; issue: "1"; pages: "59-71"
COMMENT     Sequence Label: px330-MRPsg2
FEATURES             Location/Qualifiers
     source          1..8188
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      200..209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             209..274
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             281..301
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             326..4426
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             326..4426
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4427..4474
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4502..4519)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4508..4715
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4724..4864
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4724..4853
                     /label=AAV2 ITR
     rep_origin      4939..5394
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5026..5045)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5236..5257
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5411..5430)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5530..5552
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5590..5608)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5676..5780
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5781..5849,5850..6641)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5999..6018)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6812..7400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7301..7320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7462..7710
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7462..7482
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7633..7652
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7732..7807
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        7904..8188
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
       61 ggcgggacgg cccttctcct ccgggctgta attagctgag caagaggtaa gggtttaagg
      121 gatggttggt tggtggggta ttaatgttta attacctgga gcacctgcct gaaatcactt
      181 tttttcaggt tggaccggtg ccaccatgga ctataaggac cacgacggag actacaagga
      241 tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga agcggaaggt
      301 cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg acatcggcac
      361 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
      421 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
      481 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
      541 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
      601 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
      661 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
      721 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
      781 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
      841 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
      901 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
      961 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     1021 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     1081 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     1141 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     1201 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     1261 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     1321 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     1381 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     1441 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     1501 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     1561 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     1621 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     1681 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     1741 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     1801 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     1861 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     1921 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     1981 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     2041 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     2101 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     2161 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     2221 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     2281 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     2341 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     2401 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     2461 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     2521 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     2581 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     2641 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     2701 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     2761 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     2821 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     2881 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     2941 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     3001 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     3061 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     3121 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     3181 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     3241 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     3301 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     3361 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     3421 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     3481 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     3541 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     3601 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     3661 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     3721 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     3781 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     3841 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     3901 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     3961 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     4021 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     4081 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     4141 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     4201 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     4261 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     4321 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     4381 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaaaa ggccggcggc
     4441 cacgaaaaag gccggccagg caaaaaagaa aaagtaagaa ttcctagagc tcgctgatca
     4501 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4561 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4621 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4681 gaggattggg aagagaatag caggcatgct ggggagcggc cgcaggaacc cctagtgatg
     4741 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     4801 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     4861 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4921 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4981 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     5041 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5101 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     5161 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5221 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     5281 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     5341 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     5401 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     5461 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5521 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5581 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5641 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5701 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5761 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5821 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5881 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5941 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6001 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6061 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6121 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6181 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6241 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6301 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6361 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6421 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6481 gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6541 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6601 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6661 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6721 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6781 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6841 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6901 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6961 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7021 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7081 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7141 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7201 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7261 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7321 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7381 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7441 gctggccttt tgctcacatg tgagggccta tttcccatga ttccttcata tttgcatata
     7501 cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta
     7561 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     7621 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
     7681 ttatatatct tgtggaaagg acgaaacacc gcagtgtgta gcctaggata cgttttagag
     7741 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     7801 tcggtgcttt tttgttttag agctagaaat agcaagttaa aataaggcta gtccgttttt
     7861 agcgcgtgcg ccaattctgc agacaaatgg ctctagaggt acccgttaca taacttacgg
     7921 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca atagtaacgc
     7981 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     8041 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     8101 ggcccgcctg gcattgtgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     8161 tctacgtatt agtcatcgct attaccat
//
