LOCUS       sequence-17372-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  TITLE     HOX protein coding clones not referenced in publications
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HOXC6 (human) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             105..137
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     gap             611..628
                     /estimated_length=18
     terminator      718..765
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tccctctana nnnattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatggct agcatgactg
      121 gtggacagca aatgggtcgc ggatccatgc tctcaaactg cagacaaaac accttaggac
      181 ataacacaca gacctcaatc gctcaggatt ttagttctga gcagggcagg actgcgcccc
      241 aggaccagaa agccagtatc cagatttacc cctggatgca gcgaatgaat tcgcacagtg
      301 gggtcggcta cggagcggac cggaggcgcg gccgccagat ctactcgcgg taccagaccc
      361 tggaactgga gaaggaattt cacttcaatc gctacctaac gcggcgccgg cgcatcgaga
      421 tcgccaacgc gctttgcctg accgagcgac agatcaaaat ctggttccag aaccgccgga
      481 tgaagtggaa aaaagaatct aatctcacat ccactctctc ggggggcggc ggaggggcca
      541 ccgccgacag cctgggcgga aaagaggaaa agcgggaaga gacagaagag gagaagcaga
      601 aaagagtgaa nnnnnnnnnn nnnnnnnncc cncncncccn cccccnnnnn nagatccggn
      661 nnnnntaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga ncaataacta
      721 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa ngg
//
