LOCUS       sequence-173732-        5760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of doubleTAL staple protein dT16 with N-terminally fused 
            cycle3-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pSB1A3_GFP_dT16
FEATURES             Location/Qualifiers
     source          1..5760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..714
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(31..59)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     654..674
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             5722..5739
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5740..5757
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt
      181 gtcactactt tctcttatgg tgttcaatgc ttttcccgtt atccggatca tatgaaacgg
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
      301 aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct cggacacaaa
      421 ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
      481 atcaaagcta acttcaaaat tcgccacaac attgaagatg gatccgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctgccct ttcgaaagat cccaatgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caagtggggt
      721 cttagcgcgg gcgccgtaga tttgagaact ttgggatatt cacagcagca gcaggaaaag
      781 atcaagccca aagtgaggtc gacagtcgcg cagcatcacg aagcgctggt gggtcatggg
      841 tttacacatg cccacatcgt agccttgtcg cagcaccctg cagcccttgg cacggtcgcc
      901 gtcaagtacc aggacatgat tgcggcgttg ccggaagcca cacatgaggc gatcgtcggt
      961 gtggggaaac agtggagcgg agcccgagcg cttgaggccc tgttgacggt cgcgggagag
     1021 ctgagagggc ctccccttca gctggacacg ggccagttgc tgaagatcgc gaagcgggga
     1081 ggagtcacgg cggtcgaggc ggtgcacgcg tggcgcaatg cgctcacggg agcacccctc
     1141 aacctgaccc cagagcaggt cgtggcgatc gcaagccacg acggtggcaa acaggctctg
     1201 gaaacagtac agaggctgtt gcctgtgctg tgccaagcgc acgggttgac cccagagcag
     1261 gtcgtggcga tcgcaagcca cgacggtggc aaacaggctc tggaaacagt acagaggctg
     1321 ttgcctgtgc tgtgccaagc gcacggactt accccagagc aggtcgtggc aatcgcctcc
     1381 aacattggtg gcaaacaggc tctggaaact gtccagcgcc tgcttcccgt gctgtgccaa
     1441 gcgcacggtt taaccccaga gcaggtcgtg gcaatcgcct ccaacattgg tggcaaacag
     1501 gctctggaaa ctgtccagcg cctgcttccc gtgctgtgcc aagcgcacgg gctcacccca
     1561 gagcaggtcg tggcaatcgc gagcaataac ggtggcaaac aggctctgga aacggtgcag
     1621 aggctccttc cagtgctgtg ccaagcgcac ggtctgaccc cagagcaggt cgtggcaatc
     1681 gcctccaaca ttggtggcaa acaggctctg gaaactgtcc agcgcctgct tcccgtgctg
     1741 tgccaagcgc acgggttaac cccagagcag gtcgtggcaa tcgcgagcaa taacggtggc
     1801 aaacaggctc tggaaacggt gcagaggctc cttccagtgc tgtgccaagc gcacggactc
     1861 accccagagc aggtcgtggc gatcgcaagc cacgacggtg gcaaacaggc tctggaaaca
     1921 gtacagaggc tgttgcctgt gctgtgccaa gcgcacggtt tgaccccaga gcaggtcgtg
     1981 gcaatcgcga gcaataacgg tggcaaacag gctctggaaa cggtgcagag gctccttcca
     2041 gtgctgtgcc aagcgcacgg gttgacccca gagcaggtcg tggcgatcgc aagccacgac
     2101 ggtggcaaac aggctctgga aacagtacag aggctgttgc ctgtgctgtg ccaagcgcac
     2161 ggacttaccc cagagcaggt cgtggccatt gcctcgaatg gaggtggcaa acaggctctg
     2221 gaaaccgtac aacgattgct gccggtgctg tgccaagcgc acggactgac cccagagcag
     2281 gtcgtggcaa tcgcgagcaa taacggtggc aaacaggctc tggaaacggt gcagaggctc
     2341 cttccagtgc tgtgccaagc gcacggcctg accccagagc aggtcgtggc aatcgcctcc
     2401 aacattggtg gcaaacaggc tctggaaact gtccagcgcc tgcttcccgt gctgtgccaa
     2461 gcgcacggtt taaccccaga gcaggtcgtg gccattgcct cgaatggagg tggcaaacag
     2521 gctctggaaa ccgtacaacg attgctgccg gtgctgtgcc aagcgcacgg gctcacccca
     2581 gagcaggtcg tggccattgc ctcgaatgga ggtggcaaac aggctctgga aaccgtacaa
     2641 cgattgctgc cggtgctgtg ccaagcgcac ggtctgaccc cagagcaggt cgtggcaatc
     2701 gcctccaaca ttggtggcaa acaggctctg gaaactgtcc agcgcctgct tcccgtgctg
     2761 tgccaagcgc acgggttaac cccagagcag gtcgtggcga tcgcaagcca cgacggtggc
     2821 aaacaggctc tggaaacagt acagaggctg ttgcctgtgc tgtgccaagc gcacggactc
     2881 accccagagc aggtcgtggc aatcgcctcc aacattggtg gcaaacaagc gttagagacc
     2941 gttcaacgtt tattacccgt gctgtgccaa gcgcacgggt tgaccccaga gcaggtcgtg
     3001 gccattgcct cgaatggagg tggcaaacag gctctggaaa ccgtacaacg attgctgccg
     3061 gtgctgtgcc aagcgcacgg acttacccca gagcaggtcg tggccattgc ctcgaatgga
     3121 ggtggcaaac aggctctgga aaccgtacaa cgattgctgc cggtgctgtg ccaagcgcac
     3181 ggactgagcg cgggcgccgt agatttgaga actttgggat attcacagca gcagcaggaa
     3241 aagatcaagc ccaaagtgag gtcgacagtc gcgcagcatc acgaagcgct ggtgggtcat
     3301 gggtttacac atgcccacat cgtagccttg tcgcagcacc ctgcagccct tggcacggtc
     3361 gccgtcaagt accaggacat gattgcggcg ttgccggaag ccacacatga ggcgatcgtc
     3421 ggtgtgggga aacagtggag cggagcccga gcgcttgagg ccctgttgac ggtcgcggga
     3481 gagctgagag ggcctcccct tcagctggac acgggccagt tgctgaagat cgcgaagcgg
     3541 ggaggagtca cggcggtcga ggcggtgcac gcgtggcgca atgcgctcac gggagcaccc
     3601 ctcaacctga ccccagagca ggtcgtggcc attgcctcga atggaggtgg caaacaggct
     3661 ctggaaaccg tacaacgatt gctgccggtg ctgtgccaag cgcacggttt aaccccagag
     3721 caggtcgtgg cgatcgcaag ccacgacggt ggcaaacagg ctctggaaac agtacagagg
     3781 ctgttgcctg tgctgtgcca agcgcacggg ctcaccccag agcaggtcgt ggcaatcgcc
     3841 tccaacattg gtggcaaaca ggctctggaa actgtccagc gcctgcttcc cgtgctgtgc
     3901 caagcgcacg ggttaacccc agagcaggtc gtggccattg cctcgaatgg aggtggcaaa
     3961 caggctctgg aaaccgtaca acgattgctg ccggtgctgt gccaagcgca cgggttgacc
     4021 ccagagcagg tcgtggcaat cgcgagcaat aacggtggca aacaggctct ggaaacggtg
     4081 cagaggctcc ttccagtgct gtgccaagcg cacggactta ccccagagca ggtcgtggcc
     4141 attgcctcga atggaggtgg caaacaggct ctggaaaccg tacaacgatt gctgccggtg
     4201 ctgtgccaag cgcacggact gaccccagag caggtcgtgg ccattgcctc gaatggaggt
     4261 ggcaaacagg ctctggaaac cgtacaacga ttgctgccgg tgctgtgcca agcgcacggc
     4321 ctgaccccag agcaggtcgt ggcaatcgcc tccaacattg gtggcaaaca ggctctggaa
     4381 actgtccagc gcctgcttcc cgtgctgtgc caagcgcacg gtttaacccc agagcaggtc
     4441 gtggccattg cctcgaatgg aggtggcaaa caggctctgg aaaccgtaca acgattgctg
     4501 ccggtgctgt gccaagcgca cgggctcacc ccagagcagg tcgtggcaat cgcctccaac
     4561 attggtggca aacaggctct ggaaactgtc cagcgcctgc ttcccgtgct gtgccaagcg
     4621 cacggactca ccccagagca ggtcgtggca atcgcctcca acattggtgg caaacaggct
     4681 ctggaaactg tccagcgcct gcttcccgtg ctgtgccaag cgcacggtct gaccccagag
     4741 caggtcgtgg cgatcgcaag ccacgacggt ggcaaacagg ctctggaaac agtacagagg
     4801 ctgttgcctg tgctgtgcca agcgcacggg ttgaccccag agcaggtcgt ggcaatcgcg
     4861 agcaataacg gtggcaaaca ggctctggaa acggtgcaga ggctccttcc agtgctgtgc
     4921 caagcgcacg gacttacccc agagcaggtc gtggcaatcg cgagcaataa cggtggcaaa
     4981 caggctctgg aaacggtgca gaggctcctt ccagtgctgt gccaagcgca cggactgacc
     5041 ccagagcagg tcgtggcaat cgcctccaac attggtggca aacaggctct ggaaactgtc
     5101 cagcgcctgc ttcccgtgct gtgccaagcg cacggcctga ccccagagca ggtcgtggcg
     5161 atcgcaagcc acgacggtgg caaacaggct ctggaaacag tacagaggct gttgcctgtg
     5221 ctgtgccaag cgcacggttt aaccccagag caggtcgtgg caatcgcgag caataacggt
     5281 ggcaaacagg ctctggaaac ggtgcagagg ctccttccag tgctgtgcca agcgcacgga
     5341 ctcaccccag agcaggtcgt ggcaatcgcg agcaataacg gtggcaaaca ggctctggaa
     5401 acggtgcaga ggctccttcc agtgctgtgc caagcgcacg gtttgacccc agagcaggtc
     5461 gtggcaatcg cgagcaataa cggtggcaaa caggctctgg aaacggtgca gaggctcctt
     5521 ccagtgctgt gccaagcgca cgggctcacc ccagagcagg tcgtggccat tgcctcgaat
     5581 ggaggtggca aacaggctct ggaatctatc gttgctcagc tgtcacgtcc ggacccggct
     5641 ctggctgctc tgaccaacga ccacctggtt gctctggctt gcctgggtgg tcgtccggct
     5701 atggacgctg ttaaaaaagg cctagtgccg cgcggcagcc accaccatca ccatcattaa
//
