LOCUS       sequence-173753-        3385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the two-helix-bundle shown in figure 2E,F,G of 
            associated article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexA2_2hb_ruleB
FEATURES             Location/Qualifiers
     source          1..3385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(235..339)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     407..425
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(463..485)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     585..604
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1611..1633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1622..1638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1646..1652,1653..1670,1671..1676))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1691..1712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1829..1846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2000..2588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2080..2099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2759..3385,1..165,166..234))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     join(3382..3385,1..16)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
       61 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      121 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      181 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      241 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      301 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      361 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      421 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
      481 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
      541 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
      601 tgtactgaga gtgcaccaat tgggtaccga gctcgcggcc gcaagctctg ctcgacgtaa
      661 ctgacgtata ctgtgacccg tccgttataa catgaagttg cgcagttact ccggtgaatc
      721 cacatttacc attcctatac tagaatgctc gcgtattgat ggacatagat agtccggtcc
      781 gtgttagcga tgcggaaaaa gtcgccaatg agccacttgt aacacaattg ggcatttcag
      841 atgatcgctg tcgttcctgg ttgcttacca gtacatgtat aactactcag tcagtaaata
      901 taaatgtcgg ttgccgtctt tgtccatttc gagatcaatc gatagtaaga cctatcacaa
      961 gttcgacgaa acgttacgcc tcggactaac ttcgagatct atgaattatg aacagagggt
     1021 accgcagccc tccttagcat agctagccct aagtgagctt caggccatga gacagccaag
     1081 gggtcagtcg caatagtcgg atgagcgtca gggggtagtg cgtgtgtgtt ataccaacga
     1141 ggtatcgact gtagattcgt acgtgggaga ttgtcggagc caatcctccg atataatgcc
     1201 cgggatctga ggctggaccg cggcgcttaa tacccagtgg ctcattcaat atcgattcat
     1261 ctgaatcacc cgataggtat tagaggtgcg ctgtaggctt gatagttatc atcagcggat
     1321 catctcgatc gagacaaaga tcgcacaaat tatcacgaaa ctccacaaga atagctagta
     1381 tttcatccaa taggtcgttg gatactatcg gtactactga agcctaagcc gacattgtca
     1441 ctcactaatg caggcgccag cacactctat ccaggatgga agaaactttt tgaagtcttt
     1501 acagtgataa aatcttacag tggggcaatg taatcagcgc tcttggacca aataacgaaa
     1561 tcgtgaaccg acaactcttg aacctgcttt tgctcgcttg gatccgaatt cctgtgtgaa
     1621 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1681 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1741 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1801 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1861 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1921 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1981 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2041 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2101 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2161 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2221 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2281 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2341 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2401 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2461 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2521 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2581 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2641 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2701 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2761 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2821 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2881 gtgctgcaat gataccgcga ctcccacgct caccggctcc agatttatca gcaataaacc
     2941 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3001 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3061 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3361 cttgcccggc gtcaatacgg gataa
//
