LOCUS       sequence-173757-        3322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the ring shown in figure 3C of associated article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexA2_ring
FEATURES             Location/Qualifiers
     source          1..3322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(914..936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    925..941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(949..955,956..973,974..979))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    994..1015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1132..1149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1303..1891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1383..1402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2062..2853,2854..2922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2685..2704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2923..3027)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3095..3113
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3151..3173)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3273..3292
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcggccgcaa gctctgctcg acgtaactga cgtatactgt ggaaaggcta gaacccgtcc
       61 gttataacat gaaacgagaa ctaacgatag catcggtccg gtgaatccac atttaccaac
      121 ctcgctggac atagatacta gaatgctcgc gtattgaaag gaaaaagagc aactcgtatg
      181 tccggtccgt gttagcgatg cgacgaaaac gcctccacgg tcgccaatga gccacttgta
      241 acagccggat cctcagtcag taaattcaga tgatcgctgt cgttcttgtc catgccttta
      301 tgctttacca gtacatgtat aactaagtac aagtcttact gtatatccta taaatgtcgg
      361 ttgccgtctt tgacgccaca gagggtactt cgagatcaat cgatagtaat agccctaagt
      421 gagcttcagg ccttcgacga aacgttacgc ctctcgcaat agtcggatga gcttcgagat
      481 ctatgaatta tgagttatac caacgaggta tcgatcaaga gttgtcggtt cacgaattgt
      541 cggagccatc caagagcgct gattacattg atctgaggta agattttatc actgtaaacc
      601 cagtggctca tttcttccat cctggataga gtcacccgat gcctgcatta gtgagtgaca
      661 aggcttgata gttattcagt agtaccgata gtatccgaga caatattgga tgaaatacta
      721 gctaaactcc acaagaatcg tgataatttg tgcgatctgg tcgttgatcg agatgatccg
      781 ctgatgagcc taagccgaca tacagcgcac ctctaatacc tgccagcaca ttcagatgaa
      841 tcgatattga actttttgaa gtctattaag cgccgcggtc cagcacctgc ttttgctcgc
      901 ttggatccga attcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      961 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1141 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1201 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1261 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1321 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1381 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1441 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1501 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1561 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1621 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1681 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1741 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1801 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1861 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1921 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1981 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2041 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2101 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2161 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgactcccac gctcaccggc
     2221 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2281 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2341 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2401 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2461 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2521 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2581 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2641 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2701 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2761 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2821 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2881 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2941 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3001 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3061 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3121 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3181 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3241 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     3301 gcaccaattg ggtaccgagc tc
//
