LOCUS       sequence-173788-        3558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the bent two-helix-bundle shown in figure 3B of 
            associated article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_bow_360
FEATURES             Location/Qualifiers
     source          1..3558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..52)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    41..57
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(65..71,72..89,90..95))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    110..131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(248..265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(419..1007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(499..518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1309..2103
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1363..1382)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1973..1992
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2268..2286
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2324..2346)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2446..2465
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgggcgatc gctctagagc tagcgaattc ctgtgtgaaa ttgttatccg ctcacaattc
       61 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      121 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      181 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      241 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      301 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      361 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      421 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      481 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      541 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      601 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      661 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      721 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      781 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      841 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      901 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      961 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1021 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1081 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1141 caatctaaag tatatatgag taaaaatatt ccggaattgc cagctggggc gccctctggt
     1201 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
     1261 cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     1321 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     1381 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     1441 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     1501 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     1561 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     1621 tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     1681 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     1741 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     1801 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     1861 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1921 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1981 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     2041 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     2101 tgaaccggta atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2161 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2221 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2281 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2341 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2401 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2461 ttgtactgag agtgcaccaa ttggtcgacc tcgagttaat taacgtatct gctcgacgta
     2521 actgacgtat actgtggaaa ggctagaacc cgtccgttat aacatgaaac gagaactaac
     2581 gatagcatcg gtccggtgaa tccacattta ccaacctcgc tggacataga tactagaatg
     2641 ctcgcgtatt gaaaggaaaa agagcaactc gtatgtccgg tccgtgttag cgatgcgacg
     2701 aaaacgcctc cacggtcgcc aatgagccac ttgtaacagc cggatcctca gtcagtaaat
     2761 tcagatgatc gctgtcgttc ttgtccatgc ctttatgctt taccagtaca tgtataacta
     2821 agtacaagtc ttactgtata tcctataaat gtcggttgcc gtctttgacg ccacagaggg
     2881 tacttcgaga tcaatcgata gtaatagccc taagtgagct tcaggccttc gacgaaacgt
     2941 tacgcctctc gcaatagtcg gatgagcttc gagatctatg aattatgagt tataccaacg
     3001 aggtatcgac tgtagattcg tacgtgggag attgtcggag ccaatcctcc gatataatgc
     3061 ccgggatctg agccctgcag cacacattcg ggccccagtg gctcattgct cagtgtactg
     3121 ctaaactcac ccgataggac gcccgttcca tggttgggct tgatagttaa ccgtgtatgt
     3181 ggacggtggc tggaccgcgg cgcttaataa cttgcataga actcaatatc gattcatctg
     3241 aatggcggca aaggtattag aggtgcgctg tacggcgccc acgcctcatc agcggatcat
     3301 ctcgatggca ctccagatcg cacaaattat cacgattaca gcctgactta gctagtattt
     3361 catccaatag cggccacgat actatcggta ctactgaaca ccaggctacc attgtcactc
     3421 actaatgcag gcacaatgtg actctatcca ggatggaaga atgaggactg tcagtttaca
     3481 gtgataaaat cttacccatg ctgaatgtaa tcagcgctct tggacatata ctgcatgtcg
     3541 tgaaccgaca actcttga
//
